碳纳米管在聚合物纳米纤维表面的组装及其细胞活性评价

发布时间：2018-07-10 17:18

本文选题：层层自组装 + 碳纳米管　；参考：《东华大学》2012年硕士论文

【摘要】：静电纺丝虽然拥有相当长的历史以及在组织工程领域将近30年的研究发展,但直到最近5-10年的时间里才被有效运用于组织工程和药物缓释领域。静电纺丝作为一种潜在的、具有良好运用前景的工艺手段,正引起越来越多科学工作者的兴趣和广泛研究。这主要归功于静电纺丝技术具备以下优点：简单灵活、适用性广、易于制备且直径在纳米级的纤维。在组织工程应用方面,首先,通过静电纺丝可制备出直径分布从微米级到纳米级的支架材料,纤维直径易于控制。其次,所得支架材料具有拓扑学结构和多孔性,与天然细胞外基质具有很高的相似度。第三,用于静电纺丝的原料非常丰富,包括生物可降解材料、非降解材料和天然材料。除此之外,制备得到的纤维排列方向可控：可以取向排列、或随机排列,以便调控其机械性能和生物学效应等。本研究中,我们通过静电纺丝技术与层层静电自组装技术相结合,制备出一种新型的含碳纳米管聚合物多糖支架材料并应用于组织工程。首先,将醋酸纤维素(Cellulose Acetate, CA)溶液经静电纺制备结构均一、纤维直径可控的三维纳米纤维膜；其次,通过层层静电自组装技术在醋酸纤维素纳米纤维上组装带正电荷的壳聚糖(Chitosan, CS)和带负电荷的多壁碳纳米管(Multiwalled CarbonNanotubes, MWCNTs)。对照组材料为在相同条件下组装带正电荷的壳聚糖和带负电荷的海藻酸钠(Sodium Algiate, ALG)。随后,采用扫描电镜(SEM)、红外分析(FTIR).热重分析(TGA)、机械性能测试、蛋白吸附、细胞黏附、细胞增殖、细胞形态学、溶血实验等方法对制备得到的复合多层膜支架材料进行系列表征和生物学性能评价。通过本文研究,可得到如下结论：通过层层静电自组装技术在醋酸纤维素纳米纤维上组装后成功地制备了CS/MWCNTs和CS/ALG两种支架材料。在相同的组装层数下,CS/MWCNTs支架材料的表面粗糙程度高于CS/ALG支架材料。但它们都显示良好的三维空间结构。细胞实验结果显示,CS/MWCNTs支架材料比CS/ALG支架材料更加有利于小鼠成纤维细胞L929的黏附、铺展和增殖。碳纳米管的加入改善了支架材料的机械性能和血液相容性,提高了材料对蛋白质的吸附能力、细胞的黏附和增殖能力。这些结果表明所得到的CS/MWCNTs支架材料在组织工程和再生医学具有潜在的应用价值。我们相信,通过推广这种简单易行的层层自组装技术在纤维表面进行修饰的方案,将其它具有生物活性的材料修饰到基底材料表而,可以得到一系列的具有生物医学应用价值的产品。
[Abstract]:Although electrostatic spinning has a long history and nearly 30 years of research and development in the field of tissue engineering, it has not been effectively used in the field of tissue engineering and drug delivery until the last 5-10 years. As a potential and promising process, electrostatic spinning is attracting more and more scientists' interest and extensive research. This is mainly due to the following advantages of electrostatic spinning technology: simple and flexible, wide applicability, easy to prepare and diameter in the nano-scale fibers. In the aspect of tissue engineering application, firstly, the diameter distribution of scaffold from micron to nanometer can be prepared by electrospinning, and the diameter of fiber can be easily controlled. Secondly, the scaffold has topological structure and porosity, and has high similarity with natural extracellular matrix. Third, the raw materials for electrostatic spinning are abundant, including biodegradable materials, non-degradable materials and natural materials. In addition, the prepared fibers have a controllable orientation or random alignment to regulate their mechanical properties and biological effects. In this study, a novel carbon nanotube polymer polysaccharide scaffold was prepared by combining electrostatic spinning technique with layer by layer electrostatic self-assembly technique and applied in tissue engineering. Firstly, the solution of cellulose acetate (CA) was electrospun to prepare three-dimensional nanofiber membrane with uniform structure and controllable fiber diameter. The positively charged chitosan (CS) and the negatively charged multiwalled carbon nanotubes (MWCNTs) were assembled on cellulose acetate nanofibers by layers of electrostatic self-assembly technique. In the control group, the positively charged chitosan and the negatively charged sodium alginate (ALG) were assembled under the same conditions. Then, scanning electron microscopy (SEM) and infrared analysis (FTIR) were used. Thermogravimetric analysis (TGA), mechanical properties test, protein adsorption, cell adhesion, cell proliferation, cell morphology and hemolysis test were used to characterize and evaluate the biological properties of the composite multilayer scaffolds. The conclusions are as follows: CS/ MWCNTs and CSP / ALG scaffolds were successfully fabricated by layer electrostatic self-assembly technique on cellulose acetate nanofibers. The surface roughness of CS- / MWCNTs scaffolds is higher than that of CS- / ALG scaffolds at the same number of assembly layers. But they all show good three-dimensional spatial structure. The results of cell experiment showed that CSR / MWCNTs scaffold was more favorable to the adhesion, spreading and proliferation of mouse fibroblast L929 than that of CSP / ALG scaffold. The addition of carbon nanotubes improved the mechanical properties and blood compatibility of the scaffolds, enhanced the ability of protein adsorption, cell adhesion and proliferation. These results indicate that the CS/ MWCNTs scaffolds have potential applications in tissue engineering and regenerative medicine. We believe that by popularizing this simple, layer-by-layer self-assembly technique to modify the surface of the fiber, other biologically active materials can be modified to the substrate material table. A series of products with biomedical application value can be obtained.
【学位授予单位】：东华大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

【共引文献】