基于新型PEG衍生物共聚改性聚乳酸的骨再生材料的研究
发布时间：2018-07-17 00:44
【摘要】：骨组织工程为广大骨病患者提供了新的途径和希望。其中组织再生支架材料的设计是骨组织工程成功的关键，通常要求它满足一定的力学强度、可控的降解性能和合理的表面诱导细胞增殖分化及组织再生。然而，目前很少有材料能同时具备以上性能。本研究的目的是设计一种新型的骨组织工程材料，使其集可控降解性能、一定的力学强度、适当的亲水性能及可功能化的表面等性能于一身。基于PEG衍生物聚（乙二醇-co-均苯四甲酸酐）亚胺（PAPI）与D,L-丙交（D,L-LA）酯共聚，制备了一系性能可调的PAPI-PDLLA新型共聚物。采用核磁共振（NMR）、傅立叶变换红外光谱仪（FTIR）、凝胶色谱-十八角激光散射仪（GPC-MALLS）、紫外可见光谱仪（UV）、示差扫描量热仪（DSC）、X光电子能谱（XPS）、扫描电镜（SEM）、原子力显微镜（AFM）等对共聚物的化学物理性能进行了表征；详细考察了PAPI-PDLLA共聚物的亲/疏水性、体外生物降解性能、力学性能（拉伸性能和压缩性能），以及降解过程中的力学性能变化；对PAPI-PDLLA共聚物的表面进行了氨基和羟基功能化研究；最后，评价了PAPI-PDLLA共聚物及表面功能化材料的体外细胞生物相容性。研究的主要内容和结论如下： 1.较低分子量的氨基封端的PEG（ATPEG，Mr:900）与均苯四甲酸酐（PMDA）通过高温缩聚反应合成出新型PEG衍生物P(ATPGE-co-PMDA)(PAPI)。在合成条件的优化实验中，考察了单体比例、温度、反应时间等对聚合物分子量的影响和反应过程中酰亚胺化的程度等；该衍生物通过苯酰亚胺环连接，酰亚胺环的引入为开环功能接枝提供了条件，研究了丁二胺、乙醇胺与PAPI中酰亚胺环反应的能力；对所有合成的材料结构进行了表征。 ①FTIR、1H NMR、13C NMR、GPC-MALLA和UV检测结果表明，ATPEG与PMDA成功聚合，ATPEG的微过量使得合成的PAPI端部具有氨基。当二者的摩尔比ATPEG/PMDA=1.05时，在设定的梯度高温温度下反应完成后，酰亚胺化基本完全，所获得的聚合物分子量较大，聚合物分散系数较低。热重分析表明PAPI相对于PEG的热稳定性增强。 ②FTIR表明，PAPI中酰亚胺环在室温无催化剂下成功与丁二胺和乙醇胺反应，可能为接枝功能基团提供反应位点。 ③在与丁二胺反应时，发生了交联，快速产生了凝胶，该凝胶具有一定的力学强度和多孔性，有望应用于药物释放或组织工程领域。 2.PAPI和辛酸亚锡共引发体系引发D,L-丙交酯开环，合成了一系列PAPI-PDLLA共聚物，研究了PAPI/D,L-丙交酯、反应温度、反应时间等对PAPI-PDLLA共聚物分子量的影响，并表征了其化学结构和热性能。 ①FTIR、1H NMR、13C NMR和GPC-MALLS的结果表明，PAPI的端氨基和辛酸亚锡共引发体系成功引发丙交酯开环，制备了PAPI-PDLLA共聚物，最佳反应时间为36小时，反应温度为150℃。 ②通过调节PAPI与D,L-丙交酯的物料比，可以制备一系列不同分子量和性能的共聚物，通过1H NMR计算了接枝的聚乳酸的量；随着PAPI/D,L-丙交酯比例的增加，接枝的聚乳酸分子量下降。 ③DSC结果表明，PAPI-PDLLA共聚物只有一个玻璃化转变温度，这表明两相热相容性良好，随着PAPI所占比例的增加，玻璃化温度下降。热重分析结果表明，PAPI-PDLLA出现两个明显分解温度，首先是PDLLA嵌段分解，然后是PAPI的分解，通过热重分析可以得出两嵌段的质量比。 3.研究了PAPI-PDLLA共聚物的亲/疏水性能和降解性能。亲/疏水性能采用材料表面静态水接触角和整体吸水率两种方法来评价；通过失重率、分子量变化、pH值变化和降解后样品表面形貌的变化等来评价材料的降解性能。 ①亲/疏水性能测试结果表明，PAPI-PDLLA共聚物的静态水接触角均小于PDLLA，吸水率都大于PDLLA，且随着共聚物中亲水嵌段PAPI比重的增加，亲水性能增加。 ②PAPI-PDLLA共聚物的体外降解实验表明，PAPI-PDLLA系列样品在降解前五周的失重、分子量下降及pH值变化相对于PDLLA对照组都要快些，但在整个降解过程中发现，PDLLA由于降解过程中酸性积累导致的自催化作用引起了陡降现象，而在PAPI-PDLLA系列材料中，降解速率较为可控，降解的失重率的自然对数与时间经拟合，发现符合假一级动力学模型Mnmolecular，没有陡降现象产生。这是由于亲水嵌段PAPI的引入，加速了降解的酸性小分子的扩散，没有导致材料明显的自催化降解作用。通过降解后的扫描电镜显示，PDLLA会产生局部不均匀降解，而PAPI-PDLLA共聚物的降解表面较为均一。因此，相对于PDLLA，材料的降解可控性能提高。 4.采用拉伸和压缩测试考察了材料的力学性能。结果表明，共聚物都具有一定的拉伸强度和压缩强度，并且强度随着PAPI/D,L-丙交酯的物料比减少而增加，而断裂伸长率随着PAPI/D,L-丙交酯的物料比的增加而显著增加。合成的共聚物拉伸模量在48-280MPa之间。压缩模量在108-780MPa之间，与松质骨的压缩模量较为匹配。随着材料的降解，材料的力学强度逐渐损失，且随着PAPI在共聚物中占的比重提高，力学损失加速，PAPD4/15（即PAPI-PDLLA中PAPI/D,L-LA=4/15)降解四周后，拉伸性能几乎全部损失，而PAPD4/25仅损失了20%左右。 5.采用湿法化学法，在PAPI-PDLLA共聚物膜表面引入了氨基和羟基，采用XPS、比色法、AFM等方法定性定量表征了材料表面接枝的氨基和羟基。在无催化剂、常温等反应条件下，在材料表面易引入氨基和羟基，这为后续的材料表面活性分子接枝提供了基础。PAPD4/15-BDA材料表面接枝的氨基密度达3.41×10~(-6)mol/cm~2。当接枝氨基和羟基后，材料表面相对于反应前变得粗糙。 6.采用大鼠乳鼠成骨细胞为种子细胞评价了PAPI及PAPI-PDLLA的细胞毒性。从成骨细胞形态、粘附、铺展、增殖、分化和矿化等几个方面系统的比较了PAPI-PDLLA及功能化表面与PDLLA的细胞相容性。 ①采用PAPI浸泡液及PAPI和PAPI-PDLLA降解可能产生的最大量的PMDA的浸泡液用于培养成骨细胞，发现成骨细胞在上述培养液中培养均体现正常形态，PAPI及含PMA的培养液并不影响细胞的形态与增殖，这说明了PAPI及PAPI-PDLLA无明显细胞毒性。 ②与PDLLA相比，适当的引入PAPI促进了成骨细胞的粘附与铺展，，而过多的PAPI不利于细胞的粘附；功能化的PAPI-PDLLA膜表面也有利于细胞的粘附与铺展，氨基功能化的表面细胞粘附最为明显。 ③相对于PDLLA，适当PAPI含量的PAPI-PDLLA膜有利于细胞增殖，而氨基和羟基功能化的表面使细胞增殖更为显著。 ④成骨细胞在材料表面的分化和矿化能力采用碱性磷酸酶、无机钙分泌和胶原分泌等指标来衡量。结果表明，细胞在氨基功能化的PAPI-PDLLA膜表面的分化矿化能力略优于PAPI-PDLLA，而在PAPI-PDLLA膜上的分化矿化能力要优于PDLLA。
[Abstract]:Bone tissue engineering provides a new way and hope for the patients with bone disease. The design of tissue regeneration scaffold is the key to the success of bone tissue engineering. It is usually required to meet certain mechanical strength, controllable degradation performance and reasonable surface induction of cell proliferation and differentiation and tissue regeneration. However, few materials can be used at the same time at the same time. The purpose of this study is to design a new type of bone tissue engineering material to make it capable of controlling degradation properties, certain mechanical strength, appropriate hydrophilic properties and functional surfaces. Based on the PEG derivative poly (ethylene glycol -co- benzoate anhydride) imide (PAPI), copolymerization of D, L- C (D, L-LA) ester, and copolymerization of PAPI) A series of new PAPI-PDLLA copolymers with adjustable performance are prepared. Using nuclear magnetic resonance (NMR), Fu Liye transform infrared spectrometer (FTIR), gel chromatography - eighteen angle laser scatterometer (GPC-MALLS), ultraviolet visible spectrometer (UV), differential scanning calorimeter (DSC), X photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and other copolymers The properties of chemical and physical properties were characterized. The affinity / hydrophobicity of PAPI-PDLLA copolymer, in vitro biodegradability, mechanical properties (tensile properties and compression properties), and the changes of mechanical properties during the degradation process were investigated. The amino and hydroxyl groups of the PAPI-PDLLA copolymers were studied. Finally, the PAPI-PDLLA was evaluated. Biocompatibility of copolymers and surface functionalized materials in vitro. The main contents and conclusions are as follows:
1. a new PEG derivative P (ATPGE-co-PMDA) (PAPI) was synthesized by the high temperature condensation reaction of PEG (ATPEG, Mr:900) and benzyldianhydride (PMDA) at low molecular weight, and the influence of monomer ratio, temperature and reaction time on the molecular weight of the polymer and the process of imimide during the reaction process were investigated. The derivate was connected by the benzimide ring and the introduction of the imide ring provided the conditions for the opening ring function grafting. The ability of Ding Eran, ethanolamine to react with the imide ring in PAPI was studied, and the structure of all the synthesized materials was characterized.
(1) FTIR, 1H NMR, 13C NMR, GPC-MALLA and UV detection results show that ATPEG and PMDA are successfully polymerized, and the micro excess of ATPEG makes the PAPI end have amino. When the molar ratio of the two is more than ATPEG/PMDA=1.05, after the reaction is completed at a set temperature, the imide base is complete, and the molecular weight of the polymer is larger and the polymer fraction is obtained. Thermogravimetric analysis showed that the thermal stability of PAPI relative to PEG was enhanced.
(2) FTIR showed that the imide ring in PAPI successfully reacted with butyl two amine and ethanolamine at room temperature without catalyst, which might provide a reaction site for graft functional groups.
(3) the gel was produced by crosslinking with butyl two amine, which has a certain mechanical strength and porosity, which is expected to be used in the field of drug release or tissue engineering.
The co initiation system of 2.PAPI and stannous octanate initiated a series of D, L- lactide ring opening and a series of PAPI-PDLLA copolymers. The effects of PAPI/D, L- lactide, reaction temperature and reaction time on the molecular weight of PAPI-PDLLA copolymers were investigated, and the chemical structure and thermal properties of the copolymers were characterized.
(1) the results of FTIR, 1H NMR, 13C NMR and GPC-MALLS showed that the co initiation system of PAPI and stannous octanate initiated the opening ring of lactide, and the PAPI-PDLLA copolymer was prepared. The optimum reaction time was 36 hours and the reaction temperature was 150.
(2) a series of copolymers with different molecular weights and properties can be prepared by adjusting the material ratio of PAPI and D, L- lactide. The amount of polylactic acid graft is calculated by 1H NMR. With the increase of PAPI/D, the proportion of L- lactide is increased, and the molecular weight of the grafted polylactic acid decreases.
(3) DSC results show that the PAPI-PDLLA copolymer has only one glass transition temperature, which indicates that the thermal compatibility of the two phases is good and the glass transition temperature decreases with the increase of the proportion of PAPI. The results of thermogravimetric analysis show that the PAPI-PDLLA has two obvious decomposition temperatures, first of which is PDLLA block decomposition, then the decomposition of PAPI, by thermogravimetric analysis. The mass ratio of the two block is obtained.
3. the Pro / hydrophobicity and degradation properties of PAPI-PDLLA copolymer were studied. The hydrophobicity and hydrophobicity of the copolymer were evaluated by two methods of static water contact angle and total water absorption on the surface of the material, and the degradation properties of the materials were evaluated by the weight loss rate, the change of molecular weight, the change of the pH value and the change of the surface morphology after the degradation.
The results of Pro / hydrophobicity test showed that the static water contact angle of PAPI-PDLLA copolymer was less than PDLLA, and the water absorption rate was greater than that of PDLLA, and the hydrophilic property increased with the increase of the hydrophilic block PAPI in the copolymer.
The in vitro degradation experiment of PAPI-PDLLA copolymer showed that the weight loss of PAPI-PDLLA Series in the five weeks before degradation, the decrease of molecular weight and the change of pH value were faster than that of the PDLLA control group. But in the whole degradation process, it was found that PDLLA caused a steep drop due to the autocatalytic effect of acid accumulation during the degradation process, and in PAPI-PDLLA. In the series of materials, the degradation rate is more controllable and the natural logarithm and time of the degradation weight loss are fitted. It is found that the pseudo first order dynamic model Mnmolecular has no steep drop. This is due to the introduction of the hydrophilic block PAPI, which accelerates the dispersing of the degraded acid small molecules, and does not lead to the apparent autocatalytic degradation of the material. The scanning electron microscopy (SEM) after degradation showed that PDLLA could produce local heterogeneous degradation, while the degradation surface of PAPI-PDLLA copolymer was more uniform. Therefore, the degradation control performance of the material was higher than that of PDLLA.
4. the tensile and compression tests were used to investigate the mechanical properties of the material. The results showed that the copolymer had certain tensile strength and compressive strength, and the strength increased with the reduction of the material ratio of PAPI/D, L- lactide, and the elongation at break increased significantly with the increase of the material ratio of PAPI/D and L- lactide. The compression modulus is between 48-280MPa. The compression modulus is between 108-780MPa and the compression modulus of the cancellous bone. With the degradation of the material, the mechanical strength of the material gradually loses, and with the increase of the proportion of PAPI in the copolymer, the mechanical loss accelerates, and the tensile properties are almost all after the degradation of PAPD4/15 (PAPI-PDLLA PAPI/D, L-LA=4/15) for four weeks. The loss, and PAPD4/25 only lost about 20%.
5. the amino and hydroxyl groups were introduced on the surface of PAPI-PDLLA copolymer membrane by wet chemical method. The amino and hydroxyl groups grafted on the surface of the material were qualitatively and quantitatively characterized by XPS, colorimetric method, AFM and other methods. The amino and hydroxyl groups were easily introduced on the surface of the material without catalyst and at normal temperature. The amino density of the surface grafting of the base.PAPD4/15-BDA material is 3.41 * 10~ (-6) mol/cm~2. when the grafting of the amino group and the hydroxyl group, the surface of the material becomes coarser compared with the reaction before the reaction.
6. the cytotoxicity of PAPI and PAPI-PDLLA was evaluated by rat osteoblasts of rat milk. The cytocompatibility of PAPI-PDLLA and functional surface with PDLLA was systematically compared from the aspects of osteoblast morphology, adhesion, spreading, proliferation, differentiation and mineralization.
(1) the maximum amount of PMDA immersion solution, which could be produced by PAPI immersion and PAPI and PAPI-PDLLA degradation, was used to culture osteoblasts. It was found that osteoblasts were cultured in the above culture medium, and PAPI and PMA culture medium did not affect the morphology and proliferation of the cells, which showed that there was no obvious cytotoxicity of PAPI and PAPI-PDLLA.
Compared with PDLLA, proper introduction of PAPI promotes the adhesion and spreading of osteoblasts, while excessive PAPI is not conducive to cell adhesion, and the functional PAPI-PDLLA membrane surface is also beneficial to cell adhesion and spreading, and the adhesion of amino functional surface cells is most obvious.
(3) relative to PDLLA, the PAPI-PDLLA membrane with proper PAPI content is beneficial to cell proliferation, while the functionalized surface of amino and hydroxyl groups makes cell proliferation more significant.
The differentiation and mineralization of osteoblasts on the surface of the material were measured by alkaline phosphatase, inorganic calcium secretion and collagen secretion. The results showed that the differentiation and mineralization ability of the cells on the surface of the amino functional PAPI-PDLLA membrane was slightly better than that of PAPI-PDLLA, while the mineralization ability on the PAPI-PDLLA membrane was better than that of PDLLA..
【学位授予单位】：重庆大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R318.08
本文编号：2128275
[Abstract]:Bone tissue engineering provides a new way and hope for the patients with bone disease. The design of tissue regeneration scaffold is the key to the success of bone tissue engineering. It is usually required to meet certain mechanical strength, controllable degradation performance and reasonable surface induction of cell proliferation and differentiation and tissue regeneration. However, few materials can be used at the same time at the same time. The purpose of this study is to design a new type of bone tissue engineering material to make it capable of controlling degradation properties, certain mechanical strength, appropriate hydrophilic properties and functional surfaces. Based on the PEG derivative poly (ethylene glycol -co- benzoate anhydride) imide (PAPI), copolymerization of D, L- C (D, L-LA) ester, and copolymerization of PAPI) A series of new PAPI-PDLLA copolymers with adjustable performance are prepared. Using nuclear magnetic resonance (NMR), Fu Liye transform infrared spectrometer (FTIR), gel chromatography - eighteen angle laser scatterometer (GPC-MALLS), ultraviolet visible spectrometer (UV), differential scanning calorimeter (DSC), X photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and other copolymers The properties of chemical and physical properties were characterized. The affinity / hydrophobicity of PAPI-PDLLA copolymer, in vitro biodegradability, mechanical properties (tensile properties and compression properties), and the changes of mechanical properties during the degradation process were investigated. The amino and hydroxyl groups of the PAPI-PDLLA copolymers were studied. Finally, the PAPI-PDLLA was evaluated. Biocompatibility of copolymers and surface functionalized materials in vitro. The main contents and conclusions are as follows:
1. a new PEG derivative P (ATPGE-co-PMDA) (PAPI) was synthesized by the high temperature condensation reaction of PEG (ATPEG, Mr:900) and benzyldianhydride (PMDA) at low molecular weight, and the influence of monomer ratio, temperature and reaction time on the molecular weight of the polymer and the process of imimide during the reaction process were investigated. The derivate was connected by the benzimide ring and the introduction of the imide ring provided the conditions for the opening ring function grafting. The ability of Ding Eran, ethanolamine to react with the imide ring in PAPI was studied, and the structure of all the synthesized materials was characterized.
(1) FTIR, 1H NMR, 13C NMR, GPC-MALLA and UV detection results show that ATPEG and PMDA are successfully polymerized, and the micro excess of ATPEG makes the PAPI end have amino. When the molar ratio of the two is more than ATPEG/PMDA=1.05, after the reaction is completed at a set temperature, the imide base is complete, and the molecular weight of the polymer is larger and the polymer fraction is obtained. Thermogravimetric analysis showed that the thermal stability of PAPI relative to PEG was enhanced.
(2) FTIR showed that the imide ring in PAPI successfully reacted with butyl two amine and ethanolamine at room temperature without catalyst, which might provide a reaction site for graft functional groups.
(3) the gel was produced by crosslinking with butyl two amine, which has a certain mechanical strength and porosity, which is expected to be used in the field of drug release or tissue engineering.
The co initiation system of 2.PAPI and stannous octanate initiated a series of D, L- lactide ring opening and a series of PAPI-PDLLA copolymers. The effects of PAPI/D, L- lactide, reaction temperature and reaction time on the molecular weight of PAPI-PDLLA copolymers were investigated, and the chemical structure and thermal properties of the copolymers were characterized.
(1) the results of FTIR, 1H NMR, 13C NMR and GPC-MALLS showed that the co initiation system of PAPI and stannous octanate initiated the opening ring of lactide, and the PAPI-PDLLA copolymer was prepared. The optimum reaction time was 36 hours and the reaction temperature was 150.
(2) a series of copolymers with different molecular weights and properties can be prepared by adjusting the material ratio of PAPI and D, L- lactide. The amount of polylactic acid graft is calculated by 1H NMR. With the increase of PAPI/D, the proportion of L- lactide is increased, and the molecular weight of the grafted polylactic acid decreases.
(3) DSC results show that the PAPI-PDLLA copolymer has only one glass transition temperature, which indicates that the thermal compatibility of the two phases is good and the glass transition temperature decreases with the increase of the proportion of PAPI. The results of thermogravimetric analysis show that the PAPI-PDLLA has two obvious decomposition temperatures, first of which is PDLLA block decomposition, then the decomposition of PAPI, by thermogravimetric analysis. The mass ratio of the two block is obtained.
3. the Pro / hydrophobicity and degradation properties of PAPI-PDLLA copolymer were studied. The hydrophobicity and hydrophobicity of the copolymer were evaluated by two methods of static water contact angle and total water absorption on the surface of the material, and the degradation properties of the materials were evaluated by the weight loss rate, the change of molecular weight, the change of the pH value and the change of the surface morphology after the degradation.
The results of Pro / hydrophobicity test showed that the static water contact angle of PAPI-PDLLA copolymer was less than PDLLA, and the water absorption rate was greater than that of PDLLA, and the hydrophilic property increased with the increase of the hydrophilic block PAPI in the copolymer.
The in vitro degradation experiment of PAPI-PDLLA copolymer showed that the weight loss of PAPI-PDLLA Series in the five weeks before degradation, the decrease of molecular weight and the change of pH value were faster than that of the PDLLA control group. But in the whole degradation process, it was found that PDLLA caused a steep drop due to the autocatalytic effect of acid accumulation during the degradation process, and in PAPI-PDLLA. In the series of materials, the degradation rate is more controllable and the natural logarithm and time of the degradation weight loss are fitted. It is found that the pseudo first order dynamic model Mnmolecular has no steep drop. This is due to the introduction of the hydrophilic block PAPI, which accelerates the dispersing of the degraded acid small molecules, and does not lead to the apparent autocatalytic degradation of the material. The scanning electron microscopy (SEM) after degradation showed that PDLLA could produce local heterogeneous degradation, while the degradation surface of PAPI-PDLLA copolymer was more uniform. Therefore, the degradation control performance of the material was higher than that of PDLLA.
4. the tensile and compression tests were used to investigate the mechanical properties of the material. The results showed that the copolymer had certain tensile strength and compressive strength, and the strength increased with the reduction of the material ratio of PAPI/D, L- lactide, and the elongation at break increased significantly with the increase of the material ratio of PAPI/D and L- lactide. The compression modulus is between 48-280MPa. The compression modulus is between 108-780MPa and the compression modulus of the cancellous bone. With the degradation of the material, the mechanical strength of the material gradually loses, and with the increase of the proportion of PAPI in the copolymer, the mechanical loss accelerates, and the tensile properties are almost all after the degradation of PAPD4/15 (PAPI-PDLLA PAPI/D, L-LA=4/15) for four weeks. The loss, and PAPD4/25 only lost about 20%.
5. the amino and hydroxyl groups were introduced on the surface of PAPI-PDLLA copolymer membrane by wet chemical method. The amino and hydroxyl groups grafted on the surface of the material were qualitatively and quantitatively characterized by XPS, colorimetric method, AFM and other methods. The amino and hydroxyl groups were easily introduced on the surface of the material without catalyst and at normal temperature. The amino density of the surface grafting of the base.PAPD4/15-BDA material is 3.41 * 10~ (-6) mol/cm~2. when the grafting of the amino group and the hydroxyl group, the surface of the material becomes coarser compared with the reaction before the reaction.
6. the cytotoxicity of PAPI and PAPI-PDLLA was evaluated by rat osteoblasts of rat milk. The cytocompatibility of PAPI-PDLLA and functional surface with PDLLA was systematically compared from the aspects of osteoblast morphology, adhesion, spreading, proliferation, differentiation and mineralization.
(1) the maximum amount of PMDA immersion solution, which could be produced by PAPI immersion and PAPI and PAPI-PDLLA degradation, was used to culture osteoblasts. It was found that osteoblasts were cultured in the above culture medium, and PAPI and PMA culture medium did not affect the morphology and proliferation of the cells, which showed that there was no obvious cytotoxicity of PAPI and PAPI-PDLLA.
Compared with PDLLA, proper introduction of PAPI promotes the adhesion and spreading of osteoblasts, while excessive PAPI is not conducive to cell adhesion, and the functional PAPI-PDLLA membrane surface is also beneficial to cell adhesion and spreading, and the adhesion of amino functional surface cells is most obvious.
(3) relative to PDLLA, the PAPI-PDLLA membrane with proper PAPI content is beneficial to cell proliferation, while the functionalized surface of amino and hydroxyl groups makes cell proliferation more significant.
The differentiation and mineralization of osteoblasts on the surface of the material were measured by alkaline phosphatase, inorganic calcium secretion and collagen secretion. The results showed that the differentiation and mineralization ability of the cells on the surface of the amino functional PAPI-PDLLA membrane was slightly better than that of PAPI-PDLLA, while the mineralization ability on the PAPI-PDLLA membrane was better than that of PDLLA..
【学位授予单位】：重庆大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R318.08
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