通过自生长方式实现壳聚糖—胶原聚合物引导的牙体硬组织仿生再矿化

发布时间：2018-07-17 04:46

【摘要】：目的以改良的壳聚糖、胶原等生物大分子作为矿化模板,引导釉质实现类似自生长的再矿化。方法通过对天然聚阳离子多糖——壳聚糖进行磷酸化本体改性,生成其聚阴离子衍生物磷酸化壳聚糖;以京尼平为交联剂将磷酸化壳聚糖与聚阳离子的Ⅰ型胶原交联改性,构建双性聚电解质复合物水凝胶作为生物矿化的大分子模板,以紫外光辐射激发其在惰性牙体表面的化学组装,仿生唾液提供矿化离子,调控磷灰石晶体在牙体原位的形成与组装,生成羟磷灰石。结果红外光谱检测证实,PO43-官能团(3 446 cm-1)成功引入壳聚糖,扫描电子显微镜下双性聚电解质复合物水凝胶呈现多孔并存,孔孔交通的级联结构。水凝胶原位接枝能够生成白色晶体,X射线衍射证实新生的晶体为羟磷灰石,该沉积层与原有釉质同质结合,类似釉柱平行排列;新生晶体直径30~60 nm,硬度接近牙本质。结论取材容易、操作便利的双性聚离子凝胶能够在一定程度上模仿釉质矿化模板,实现羟磷灰石的自生长,这对釉质结构仿生的发展有重要意义。
[Abstract]:Objective to use modified chitosan, collagen and other biological macromolecules as mineralization templates to guide enamel to realize self-growth-like re-mineralization. Methods Phosphorylated chitosan was synthesized by phosphorylation of natural polycationic polysaccharide-chitosan, and was crosslinked with polycationic collagen type I by using genipin as crosslinking agent. The bisexual polyelectrolyte complex hydrogel was constructed as a macromolecular template for biomineralization, and its chemical assembly on the surface of inert teeth was stimulated by ultraviolet radiation, and the biomimetic saliva provided mineralized ions. The formation and assembly of apatite crystals in situ to form hydroxyapatite are regulated. Results IR spectra showed that the chitosan was successfully introduced into PO43- functional group (3 446 cm-1). Under scanning electron microscope (SEM), the polyelectrolyte complex hydrogels were porous, and the pore traffic was in a cascade structure. In situ grafting of hydrogels into white crystals confirmed that the new crystals were hydroxyapatite, which combined with the original enamel and was parallel to the enamel column, and the diameter of the new crystals was 3060 nm and the hardness was close to that of dentin. Conclusion the bisexual polyionic gel, which is easy to use and easy to operate, can mimic the enamel mineralization template to some extent and realize the self-growth of hydroxyapatite, which is of great significance to the development of enamel bionic structure.
【作者单位】：四川省医学科学院.四川省人民医院口腔科;遵义医学院口腔学院;
【基金】：国家自然科学基金资助项目(81100786) 四川省科技支撑计划基金资助项目(2013SZ0020)
【分类号】：R783.1

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