蚕丝-PLGA网状支架的力学强度检测及其与骨髓间充质干细胞共培养

发布时间：2018-07-20 19:40

【摘要】：目的进行蚕丝-PLGA复合网状支架的力学强度检测及其与骨髓间充质干细胞的体外共培养。方法采用纬编针织法制备蚕丝丝素-PLGA纤维复合网状支架,并进行力学强度测试。在网状支架上滴加Ⅰ型胶原并冷冻干燥,再用纤维连接蛋白表面修饰后,种植兔骨髓间充质干细胞进行体外共培养,观察细胞与支架复合生长情况。结果蚕丝-PLGA网状支架宽约5 mm。支架的最大载荷、抗拉强度、断点伸长率、弹性模量分别为(118.32±11.21)N、(52.90±5.03)MPa、49.79%±5.16%、(177.25±19.18)MPa。支架-MSCs复合物培养2 d的扫描电镜观察显示:MSCs细胞黏附于支架表面呈立体状生长,细胞呈梭形,增殖良好,并分泌基质。结论蚕丝-PLGA复合网状支架具有良好的力学性能与细胞相容性,是一种较有潜力的组织工程韧带的支架材料。
[Abstract]:Objective to investigate the mechanical strength of silk-PLGA composite reticular scaffold and co-culture with bone marrow mesenchymal stem cells in vitro. Methods the silk fibroin-PLGA fiber composite mesh scaffold was prepared by weft knitting method and its mechanical strength was tested. The mesenchymal stem cells of rabbit bone marrow were co-cultured with fibronectin after adding type I collagen and freeze-drying on the reticular scaffold. The growth of the cells and scaffolds was observed. Results the width of silk-PLGA mesh scaffold was about 5 mm. The maximum load, tensile strength, elongation at breakpoint and elastic modulus of the scaffold were (118.32 卤11.21) N, (52.90 卤5.03) MPA, (49.79% 卤5.16) and (177.25 卤19.18) MPA, respectively. SEM observation of scaff-MSCs complex culture for 2 days showed that the cells adhered to the scaffold were stereoscopic, the cells were fusiform, proliferated well, and secreted matrix. Conclusion Silkworm filament-PLGA composite reticular scaffold has good mechanical properties and cytocompatibility and is a potential scaffold for tissue engineering ligament.
【作者单位】：浙江省医学科学院生物工程研究所;
【基金】：国家自然科学基金面上项目(81071467) 浙江省自然科学基金(LY13H060004) 浙江省医学重点学科群项目生物医药重点学科群(XKQ-010-001) 浙江省科技计划项目(2013F10006) 浙江省医药卫生科技计划项目(2014ZDA005,2013KYA045)
【分类号】：R318.08

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