壳聚糖微球包裹幽门螺杆菌全菌蛋白抗原的制备及体外释放性能（英文）

发布时间：2018-07-22 11:41

【摘要】：背景:包裹幽门螺杆菌全菌蛋白抗原的研究仍处于探索阶段,有关壳聚糖微球包裹幽门螺杆菌全菌蛋白抗原的制备工艺及体外释放性能的文献甚少。目的:探讨幽门螺杆菌全菌蛋白抗原壳聚糖微球的制备工艺及体外释放特性。方法:采用沉淀法制备壳聚糖微球,筛选最佳制备工艺及配比、包裹时间,并在电镜下观察微球的形态和粒径。采用壳聚糖微球包裹幽门螺杆菌全菌蛋白抗原,BCA法测定幽门螺杆菌全菌蛋白抗原微球的包裹率、包裹量及体外释放率。结果与结论:终体积分数为1%的冰醋酸、硫酸钠为交联剂、pH 5.0、滴加交联剂时不粉碎处理为壳聚糖微球最佳制备工艺,电镜观察显示微球表面光滑、形态圆整,具有良好的分散性,多数微球粒径为1.0-5.0μm。幽门螺杆菌全菌蛋白抗原微球的包裹率为80.4%,包裹量为16.4%,48 h总释放率为19.4%,幽门螺杆菌全菌蛋白抗原微球整体呈缓慢释放状态。结果证实,实验制备的壳聚糖微球对幽门螺样菌全菌蛋白抗原具有良好的包裹率和包裹量,幽门螺杆菌全菌蛋白抗原微球整体呈缓慢释放状态。
[Abstract]:Background: the study of the whole protein antigen of Helicobacter pylori is still in the exploratory stage. There are few literatures about the preparation of the protein antigen of Helicobacter pylori wrapped by chitosan microspheres and its release in vitro. Aim: to study the preparation and release characteristics of Helicobacter pylori protein antigen chitosan microspheres. Methods: chitosan microspheres were prepared by precipitation method. The optimum preparation technology and ratio, the encapsulation time and the morphology and particle size of chitosan microspheres were observed under electron microscope. The encapsulation rate, encapsulation quantity and in vitro release rate of Helicobacter pylori whole protein antigen microspheres were determined by chitosan microspheres. Results and conclusion: 1% final volume fraction of glacial acetic acid, sodium sulfate as crosslinking agent (pH 5.0), no comminution when adding crosslinking agent, was the best preparation technology of chitosan microspheres. Electron microscope observation showed that the surface of microspheres was smooth and the shape was round. The particle size of most microspheres was 1.0-5.0 渭 m. The encapsulation rate of the whole protein antigen microspheres of Helicobacter pylori was 80.4 and the total release rate of the whole protein antigen microspheres of Helicobacter pylori in 48 hours was 19.4.The whole microsphere of Helicobacter pylori protein antigen was slowly released. The results showed that the prepared chitosan microspheres had a good encapsulation rate and encapsulation capacity to the whole protein antigen of Helicobacter pylori, and the whole protein microspheres of Helicobacter pylori showed a slow release state.
【作者单位】：青岛大学医学院;青岛大学附属医院消化内科;
【分类号】：R318.08

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