微波辅助聚氨酯表面改性及其血液相容性研究

发布时间：2018-08-07 09:33

【摘要】：为了改善聚氨酯的血液相容性能，本文主要研究了利用微波辅助表面接枝改性的方法，对聚氨酯的表面进行活化并接枝上不同分子量的聚乙二醇（PEG/MPEG/APEG）以及肝素，利用傅里叶变换衰减全反射红外光谱法、表面水接触角、扫描电镜和原子力显微镜对聚氨酯表面特征进行分析，并通过溶血率、凝血酶原时间、补体激活等测试考察了聚氨酯表面的血液相容性能。采用微波辅助技术将聚氨酯膜的表面进行活化。结果表明：微波辅助反应能明显加快聚氨酯表面活化的速度，，其接枝效果受微波功率、反应时间以及反应物体积质量分数的影响。在本实验条件下，微波功率为100W，反应物的质量分数为5%，反应时间为10分钟时，微波辅助表面活化的效果以及效益最好。SEM表面分析表明，与传统加热反应方法相比，微波辅助反应对膜表面的破坏程度更小。采用微波辅助技术在聚氨酯膜的表面接枝不同分子量的PEG/MPEG，并与传统加热接枝PEG/MPEG的方法进行对比。通过ATR-FTIR、接触角分析不同方法改性后聚氨酯表面特性的变化，通过SEM、AFM观察不同改性方法对聚氨酯表面形貌的影响，通过溶血率、血小板黏附测试、凝血时间以及补体激活测试考察不同改性方法对聚氨酯的血液相容性能的影响。结果表明：微波辅助反应能明显加快聚氨酯表面接枝PEG/MPEG的速度，但对接枝量没有太大的影响。SEM和AFM表面分析表明，与传统加热反应方法相比，微波辅助反应对膜表面的破坏程度更小，膜表面更加光滑平整。水接触角的结果也表明，微波辅助反应接枝PEG/MPEG所得膜表面具有良好的亲水性能。血液相容性试验证实，利用微波辅助改性的聚氨酯表面呈现出更加优异的血液相容性。采用微波辅助技术将聚氨酯表面活化后，再将APEG接枝到聚氨酯上，最后利用EDC·HCl和NHS活化接枝上肝素。通过聚氨酯表面肝素化后性能的改变以及血液相容性能的改善来研究肝素对聚氨酯表面性能的影响。血液相容性测试结果证实肝素对材料血液相容性有明显的改善作用。
[Abstract]:In order to improve the blood compatibility of polyurethane, the surface of polyurethane was activated and grafted with poly (ethylene glycol) (PEG/MPEG/APEG) and heparin (heparin) with different molecular weight by microwave assisted surface grafting. Fourier transform attenuated total reflectance infrared spectroscopy (FTIR), surface water contact angle, scanning electron microscope (SEM) and atomic force microscope (AFM) were used to analyze the surface characteristics of polyurethane. The blood compatibility of polyurethane surface was investigated by complement activation test. The surface of polyurethane film was activated by microwave assisted technique. The results showed that microwave-assisted reaction could accelerate the activation of polyurethane surface, and the grafting effect was influenced by microwave power, reaction time and mass fraction of reactants. Under the experimental conditions, microwave power is 100 W, mass fraction of reactant is 5, reaction time is 10 minutes, the effect and benefit of microwave assisted surface activation is the best. SEM surface analysis shows that compared with traditional heating reaction method, microwave assisted surface activation is better than conventional heating reaction method. The microwave assisted reaction has less damage to the film surface. The microwave-assisted technique was used to graft PEG- / MPEGon with different molecular weights on the surface of polyurethane membrane and compared with the conventional heating grafted PEG/MPEG. The surface properties of polyurethane modified by different methods were analyzed by ATR-FTIR.The effects of different modification methods on the surface morphology of polyurethane were observed by SEMAFM. The hemolysis rate and platelet adhesion were measured. The effects of different modification methods on the blood compatibility of polyurethane were investigated by coagulation time and complement activation test. The results show that the microwave-assisted reaction can obviously accelerate the grafting rate of PEG/MPEG on polyurethane surface, but the grafting amount has no great influence on AFM surface analysis. The microwave assisted reaction has less damage to the film surface, and the film surface is more smooth and smooth. The results of water contact angle also showed that the surface of the membrane prepared by microwave-assisted reaction grafted with PEG/MPEG had good hydrophilicity. Hemocompatibility test confirmed that the surface of polyurethane modified by microwave showed better blood compatibility. The surface of polyurethane was activated by microwave-assisted technique, then APEG was grafted onto polyurethane. Finally, EDC HCl and NHS were used to activate the grafted heparin. The effects of heparin on the surface properties of polyurethane were studied through the changes of its properties after heparinization and the improvement of its blood compatibility. The results of blood compatibility test confirmed that heparin had a significant effect on improving the blood compatibility of the materials.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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