超声雾化喷涂法制备抗血小板膜糖蛋白单克隆抗体洗脱支架及其生物相容性研究

发布时间：2018-08-07 11:22

【摘要】：采用超声雾化喷涂法制备抗血小板膜糖蛋白单克隆抗体洗脱支架。以涂层均匀度和接触角大小来优化壳聚糖单抗涂层参数,在外层喷涂一层左旋聚乳酸作为屏蔽层控制单抗SZ-21的释放速度。通过溶血率检测涂层支架的血液相容性;MTT实验和细胞粘附实验考察涂层支架的细胞相容性。对支架表面涂层进行SEM和EDS分析,结果表明,聚合物和单抗成功涂层到支架表面,且涂层均匀。涂层支架溶血率低于国家标准的5%,涂层支架对血管内皮细胞没有产生明显的毒副作用;涂层支架有良好的细胞相容性,利于细胞在支架上生长。具有屏蔽层的单抗SZ-21涂层支架较没有涂层屏蔽层的单抗SZ-21涂层支架的SZ-21释放明显成缓慢释放,无暴释现象。
[Abstract]:The anti-platelet membrane glycoprotein monoclonal antibody eluting scaffold was prepared by ultrasonic spray spraying. The coating parameters of chitosan McAb were optimized by coating uniformity and contact angle. The release rate of SZ-21 was controlled by spraying a layer of L-polylactic acid on the outer layer. The cytocompatibility of the coated scaffold was investigated by MTT assay and cell adhesion test. The results of SEM and EDS analysis showed that the polymer and McAb were successfully coated on the surface of the scaffold and the coating was uniform. The hemolysis rate of the coated scaffold was lower than that of the national standard. The coated scaffold had no obvious toxic and side effects on the vascular endothelial cells, and the coated scaffold had good cytocompatibility and was conducive to the growth of the cells on the scaffold. The release of SZ-21 in the SZ-21 coated scaffold with shielding layer was slower than that in the SZ-21 coated scaffold without the coating shield layer, and there was no burst release phenomenon.
【作者单位】：重庆大学生物工程学院重庆市血管植入物工程实验室生物流变科学与技术教育部重点实验室;
【基金】：国家科技支撑计划资助项目(2012BAI18B02) 国家自然科学基金资助项目(11332003) 国家“111计划”资助项目(B06023)
【分类号】：R318.08

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