温度对血清荧光产生效率的影响
发布时间:2018-08-08 14:28
【摘要】:从实验的角度探讨了荧光产生效率与温度的依赖关系,实验中用不同波长激发光在不同温度下激发血清,研究了血清荧光强度随温度的变化规律。实验结果表明:当温度在28~33℃范围内变化时,激发的血清荧光强度随着温度的增加荧光强度减小。当温度在35~41℃范围内变化时,荧光强度随温度增加不再是线性减小,而是出现跳跃式变化。当用320~350nm激发光激发血清时,随着温度的增加荧光强度接近线性减小。当用其它波长的光激发血清时,荧光强度并不随温度线性地减小,研究为探测血清荧光光谱时样品的温度选择提供了实验依据。
[Abstract]:From the point of view of experiment, the dependence of fluorescence production efficiency on temperature was discussed. In the experiment, different wavelengths of excitation light were used to excite the serum at different temperatures, and the variation of serum fluorescence intensity with temperature was studied. The experimental results show that the fluorescence intensity of the stimulated serum decreases with the increase of temperature when the temperature varies in the range of 2833 鈩,
本文编号:2172077
[Abstract]:From the point of view of experiment, the dependence of fluorescence production efficiency on temperature was discussed. In the experiment, different wavelengths of excitation light were used to excite the serum at different temperatures, and the variation of serum fluorescence intensity with temperature was studied. The experimental results show that the fluorescence intensity of the stimulated serum decreases with the increase of temperature when the temperature varies in the range of 2833 鈩,
本文编号:2172077
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