改性PLGA用做载药粒子以及组织工程支架材料

发布时间：2018-08-11 12:55

【摘要】：首先,本文使用甲氧基聚乙二醇(mPEG)引发乙交酯和丙交酯开环共聚生成mPEG-PLGA,通过对聚合物的端羟基羧基化,羧基活化,接枝胶原片段(Dcol).对接枝胶原片段的聚合物进行一系列的表征,包括核磁(1H-NMR),高压液相色谱(GPC),傅里叶变换红外光谱(FTIR),元素分析。结果证明胶原片段(Dcol)和mPEG-PLGA接枝成功。本文将接有胶原片段的聚合物制备成胶束,并对胶束进行了表征,实验结果显示,临界胶束浓度(CMC)是5×10-6g/ml,动态光散射(DLS)测得的平均粒径150nm,并用扫描电镜对胶束形貌进行观察,表明聚合物mPEG-PLGA-Dcol能够形成稳定的球形胶束。本文应用聚合物的胶束包裹油溶性药物利福平,担载药物同时观察药物缓释行为,并研究了药物的包封率,结果表明,mPEG-PLGA-Dcol与mPEG-PLGA相比包封率增加,而且没有突释。另外,本文对异丙醇引发的聚乳酸(IPA-PLA)与异丙醇引发乙交酯与丙交酯开环共聚接枝胶原片段(IPA-PLGA-Dcol)混合物进行静电纺丝研究,在前期合成的基础上,将IPA-PLGA-Dcol混在IPA-PLA中进行纺丝,得到了直径很均匀的纺丝毛毡,纤维丝的直径大概为700nm。并对混合的聚合物进行了用二次升温曲线(DSC),以及热失重(TG)等表征,最后证明IPA-PLGA-Dcol的加入没有影响IPA-PLA的热性能,因此还可以用熔融加工的方式制备组织工程支架。
[Abstract]:Firstly, methoxy polyethylene glycol (mPEG) was used to initiate ring-opening copolymerization of glycolide and lactide to form mPEG-PLGA. The poly (ethylene glycol) (Dcol). Was synthesized by hydroxyl carboxylation, activation of carboxyl group and graft of collagen fragment (Dcol). A series of polymer grafted collagen fragments were characterized, including nuclear magnetic resonance (1H-NMR), high pressure liquid chromatography (HPLC) (GPC), Fourier transform infrared spectroscopy (FTIR),) element analysis. The results showed that collagen fragments (Dcol) and mPEG-PLGA were grafted successfully. In this paper, the polymer with collagen fragment was prepared and characterized. The experimental results show that the critical micelle concentration (CMC) is 5 脳 10 ~ (-6) g / ml, the average particle size measured by dynamic light scattering (DLS) is 150 nm, and the morphology of the micelle is observed by scanning electron microscope. It is shown that polymer mPEG-PLGA-Dcol can form stable spherical micelles. In this paper, polymer micelle encapsulated rifampicin was used to observe the drug release behavior, and the encapsulation efficiency of the drug was studied. The results showed that the encapsulation efficiency of mPEG-PLGA-Dcol was higher than that of mPEG-PLGA, and there was no sudden release. In addition, the mixture of polylactic acid (IPA-PLA) initiated by isopropanol and glycolide initiated by isopropanol and lactide graft collagen fragment (IPA-PLGA-Dcol) was studied by electrospinning. On the basis of the previous synthesis, IPA-PLGA-Dcol was mixed into IPA-PLA for spinning. The spinning felt with a uniform diameter was obtained, and the diameter of the fiber was about 700 nm. The mixed polymers were characterized by secondary heating curve (DSC), and thermogravimetric (TG). Finally, it was proved that the addition of IPA-PLGA-Dcol had no effect on the thermal properties of IPA-PLA, so the scaffolds could be fabricated by melt processing.
【学位授予单位】：长春理工大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：TQ320.1;R318.08

【相似文献】