MgZnMn和MgZnRe合金的体外生物相容性评价
发布时间：2018-08-30 16:43
【摘要】：生物可降解支架的研究已经成为当今心血管介入治疗的重点发展方向。支架的如期降解可以克服支架自身的血栓源性及异物性。而可降解支架中,由于镁和镁合金的生物可降解性和良好的生物相容性,使得镁和镁合金可降解支架受到材料学和医学界的广泛关注。然而,由于镁和镁合金的化学性质活泼,耐蚀性较差,阻碍了镁和镁合金可降解支架的研究发展。 本文主要是对课题组自行熔炼制备的镁锌锰(MgZnMn)合金和镁锌稀土(MgZnRe)合金进行初步的生物相容性研究,为后期的MgZnMn合金和MgZnRe合金的性能改进、表面改性等提供参考。 本文通过析氢腐蚀实验评价了MgZnMn、MgZnRe以及商用AZ31B镁合金的耐腐蚀性能。采用体外血小板粘附,凝血时间以及溶血率测试评价了三种合金的血液相容性。利用细胞凋亡染色、细胞增殖实验和细胞计数等方法评价了三种镁合金的细胞相容性。 由实验数据得到以下结论：析氢腐蚀实验结果表明,MgZnMn合金和MgZnRe合金的耐腐蚀性能基本相当,且都明显优于商用的AZ31B镁合金。血液相容性结果显示,与AZ31B合金和316L SS样品相比较,MgZnMn合金和MgZnRe合金表面粘附血小板数量较少且基本无团聚现象。而MgZnMn合金和MgZnRe合金的凝血时间与血浆相比也有明显的延长,同时MgZnMn和MgZnRe的溶血率分别为4.7%、4.4%符合相关的国家标准,且显著低于AZ31B合金的80.9%,因此MgZnMn和MgZnRe合金具有较好的血液相容性。细胞相容性评价的细胞凋亡染色(A0/PI染色)、细胞增殖评价(CCK-8)、细胞计数结果都一致表明：MgZnMn合金和MgZnRe合金的细胞相容性明显优于商用AZ31B合金。
[Abstract]:Biodegradable stents have become the focus of cardiovascular interventional therapy. Stent degradation on time can overcome the thrombogenic and foreign body properties of stent itself. Because of the biodegradability and good biocompatibility of magnesium and magnesium alloys, the biodegradable scaffolds of magnesium and magnesium alloys have attracted wide attention in the field of materials and medicine. However, the chemical activity and corrosion resistance of magnesium and magnesium alloys hinder the research and development of magnesium and magnesium alloy degradable scaffolds. In this paper, the biocompatibility of mg, Zn, mn, (MgZnMn) and Mg-Zn rare earth (MgZnRe) alloys prepared by our own melting is studied, which provides a reference for the improvement of properties and surface modification of MgZnMn and MgZnRe alloys. The corrosion resistance of MgZnMn,MgZnRe and commercial AZ31B magnesium alloy was evaluated by hydrogen evolution corrosion test. In vitro platelet adhesion, coagulation time and hemolysis rate were used to evaluate the blood compatibility of the three alloys. The cytocompatibility of three magnesium alloys was evaluated by cell apoptosis staining, cell proliferation test and cell count. The results of hydrogen evolution corrosion test show that the corrosion resistance of mg Zn mn alloy and MgZnRe alloy are basically the same, and both of them are obviously superior to commercial AZ31B magnesium alloy. The results of blood compatibility showed that compared with the samples of AZ31B and 316L SS, the number of platelet adhesion on the surface of Mg-ZnMn alloy and MgZnRe alloy was less and there was almost no aggregation. The coagulation time of MgZnMn alloy and MgZnRe alloy was significantly longer than that of plasma, and the hemolysis rate of MgZnMn and MgZnRe were 4.7% and 4.4% respectively, which were lower than that of AZ31B alloy 80.9%. Therefore, MgZnMn and MgZnRe alloy had better blood compatibility. The results of apoptosis staining (A0/PI staining), cell proliferation evaluation (CCK-8) and cell counting showed that the cytocompatibility of MgZnRe alloy and MgZnRe alloy was significantly better than that of commercial AZ31B alloy.
【学位授予单位】：西南交通大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R318.08
本文编号：2213665
[Abstract]:Biodegradable stents have become the focus of cardiovascular interventional therapy. Stent degradation on time can overcome the thrombogenic and foreign body properties of stent itself. Because of the biodegradability and good biocompatibility of magnesium and magnesium alloys, the biodegradable scaffolds of magnesium and magnesium alloys have attracted wide attention in the field of materials and medicine. However, the chemical activity and corrosion resistance of magnesium and magnesium alloys hinder the research and development of magnesium and magnesium alloy degradable scaffolds. In this paper, the biocompatibility of mg, Zn, mn, (MgZnMn) and Mg-Zn rare earth (MgZnRe) alloys prepared by our own melting is studied, which provides a reference for the improvement of properties and surface modification of MgZnMn and MgZnRe alloys. The corrosion resistance of MgZnMn,MgZnRe and commercial AZ31B magnesium alloy was evaluated by hydrogen evolution corrosion test. In vitro platelet adhesion, coagulation time and hemolysis rate were used to evaluate the blood compatibility of the three alloys. The cytocompatibility of three magnesium alloys was evaluated by cell apoptosis staining, cell proliferation test and cell count. The results of hydrogen evolution corrosion test show that the corrosion resistance of mg Zn mn alloy and MgZnRe alloy are basically the same, and both of them are obviously superior to commercial AZ31B magnesium alloy. The results of blood compatibility showed that compared with the samples of AZ31B and 316L SS, the number of platelet adhesion on the surface of Mg-ZnMn alloy and MgZnRe alloy was less and there was almost no aggregation. The coagulation time of MgZnMn alloy and MgZnRe alloy was significantly longer than that of plasma, and the hemolysis rate of MgZnMn and MgZnRe were 4.7% and 4.4% respectively, which were lower than that of AZ31B alloy 80.9%. Therefore, MgZnMn and MgZnRe alloy had better blood compatibility. The results of apoptosis staining (A0/PI staining), cell proliferation evaluation (CCK-8) and cell counting showed that the cytocompatibility of MgZnRe alloy and MgZnRe alloy was significantly better than that of commercial AZ31B alloy.
【学位授予单位】：西南交通大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R318.08
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