Galectin-8蛋白对组织工程心脏瓣膜种子细胞的增殖、黏附功能的影响

发布时间：2018-09-01 05:59

【摘要】：目的体外研究Galectin-8在不同浓度或状态下对组织工程心脏瓣膜种子细胞增殖及黏附的影响。方法以骨髓间充质干细胞分化而来的内皮细胞作为种子细胞,离体状态下常规培养。采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法测定不同浓度下Galectin-8作用12、24、48 h后对细胞增殖的影响;将细胞分为重组质粒p GFP-Galectin-8转染组、空质粒p GFP转染组、阴性对照组及蛋白预铺组,分别铺于96孔板中,观察相同时间段的细胞黏附数量。结果 Galectin-8 10、20、40μg/ml剂量组的细胞增殖数目均显著高于空白对照组,其中20μg/ml组表现尤为显著(P0.05);p GFP-Galectin-8转染组的转染效率与空质粒p GFP转染组无显著差异(P0.05),且Galectin-8的表达量显著高于空质粒p GFP转染组和阴性对照组(P0.05);细胞种植到培养板上1、2、4 h后,蛋白预铺组相同时间的细胞黏附数量显著高于其他各组(P0.05),p GFP-Galectin-8转染组的细胞黏附数量显著小于其他各组(P0.05),空质粒p GFP转染组和阴性对照组的细胞黏附数量无显著差异(P0.05)。结论 Galectin-8对内皮细胞的增殖具有促进作用,且在细胞种植前预铺蛋白固定后能够显著增加内皮细胞的黏附,而胞内Galectin-8过表达则抑制细胞黏附。
[Abstract]:Objective to study the effect of Galectin-8 on the proliferation and adhesion of tissue engineered heart valve seed cells at different concentrations or in vitro. Methods Endothelial cells derived from bone marrow mesenchymal stem cells were used as seed cells and cultured in vitro. The effects of different concentrations of Galectin-8 on cell proliferation were determined by 3- (4- (4-) -dimethylthiazol-2) -2- (diphenyltetrazolium bromide) (MTT) method. The cells were divided into three groups: recombinant plasmid p GFP-Galectin-8 transfection group, empty plasmid p GFP transfection group, negative control group and protein precoated group. The cell adhesion was observed in 96-well plate at the same time. Results the number of cell proliferation in the Galectin-8 10 ~ 20 ~ 20 渭 g/ml group was significantly higher than that in the blank control group. The transfection efficiency of 20 渭 g/ml group was especially significant (P0.05), and the expression of Galectin-8 was significantly higher than that of empty plasmid p GFP transfection group and negative control group (P0.05), and the expression of Galectin-8 was significantly higher than that of empty plasmid p GFP transfection group and negative control group (P0.05). The number of cell adhesion in the protein precoated group at the same time was significantly higher than that in the other groups (P0.05), but there was no significant difference between the empty plasmid p GFP transfection group and the negative control group (P0.05). Conclusion Galectin-8 can promote the proliferation of endothelial cells and increase the adhesion of endothelial cells after immobilization of precoated protein before implantation, while the overexpression of intracellular Galectin-8 inhibits the adhesion of endothelial cells.
【作者单位】：河南省人民医院;
【分类号】：R318.11;R54

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