血管基质成分联合脱细胞骨基质-壳聚糖支架修复桡骨缺损

发布时间：2018-09-01 09:14

【摘要】：背景:研究表明,复合使用人工组织工程骨材料和脱细胞骨基质做为骨缺损修复的支架材料,可以综合两者的优点。目的:进一步验证脂肪组织基质血管基质性成分联合脱细胞骨基质壳聚糖支架复合物修复兔桡骨缺损的安全性和生物相容性。方法:取38只新西兰大白兔,其中3只用于脂肪组织血管基质成分的制备,3只用于脱细胞骨基质的制备,32只分为实验组和对照组,采用Brownlow法制备桡骨缺损型,实验组移植血管基质成分-脱细胞骨基质壳聚糖复合支架,对照组造模后不做任何处理。术后2,4个月分别进行一般情况和大体观察、X射线拍照、病理观察、Lane-Sandhu组织学评分。结果与结论:1术后2,4个月2组无感染现象,但实验组的活动量和愈合程度显著性好于对照组;2术后2个月实验组骨缺损部位的X射线影像结果有显著灰白色的高密度影,术后4个月与正常的桡骨骨组织相同,对照组骨不连接;3术后2,4个月实验组病理显示骨组织生长情况显著性的高于对照组,Lane-Sandhu组织学评分显著性的高于对照组(P0.05);4结果说明,血管基质成分-脱细胞骨基质壳聚糖复合支架修复兔桡骨缺损具有很好的安全性和生物相容性。
[Abstract]:Background: studies have shown that the composite use of artificial tissue engineering bone materials and acellular bone matrix as scaffold materials for bone defect repair can integrate the advantages of both. Aim: to further verify the safety and biocompatibility of adipose tissue matrix vascular matrix composition combined with acellular bone matrix chitosan scaffold for repairing rabbit radius defect. Methods: 38 New Zealand white rabbits were divided into experimental group and control group. Among them, 3 rabbits were used for the preparation of vascular matrix in adipose tissue. 32 rabbits were divided into experimental group and control group. Radial defect type was prepared by Brownlow method. The experimental group was implanted with acellular bone matrix chitosan composite scaffold, while the control group did not do any treatment after modeling. The general and gross observations were taken 2 and 4 months after operation, and the histological score of Lane-Sandhu was observed by pathology. Results and conclusion there was no infection in the two groups at 2 and 4 months after operation, but the activity and healing degree of the experimental group was significantly better than that of the control group 2 months after operation. At 4 months after operation, the radial bone tissue of the control group was the same as that of the normal radial bone tissue. The pathological tissue growth of the experimental group was significantly higher than that of the control group (P0.05) at 2 and 4 months after the operation, and the histological score of the control group was significantly higher than that of the control group (P0.05). It is safe and biocompatible to repair rabbit radius defect with vascular matrix component acellular bone matrix chitosan composite scaffold.
【作者单位】：解放军第四五五医院骨科;解放军第四五五医院生物诊疗科;解放军第四五五医院整形外科;
【基金】：全军医药卫生科研项目(14ZD09)~~
【分类号】：R318.08;R683

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