天然高分子基可注射水凝胶结构调控及细胞支架应用
发布时间：2021-09-04 06:31
多种多样的聚合物被潜在地广泛应用于组织再生生物材料。可注射的水凝胶在生物医学领域的应用十分广泛:如细胞支持支架和治疗细胞以及各种药物的输送系统。这些材料在再生医学领域的多项研究中已证明具有理想的性能。先前的研究表明,衍生自天然聚合物的水凝胶具有出色的生物相容性、生物活性、生物降解性和生物利用度。水凝胶能够借助其3D网络结构从而吸收大量的水,这一特性使水凝胶能够拥有与ECM具有相似特性的基质,因此它们能够提供细胞友好的环境以助其附着,生长和增殖。细胞死亡过程可以自然发生,也可以由于各种原因在人体内发生。受损的细胞和组织由于失去功能而无法正常地执行其功能。自然,如果根本原因是细胞自然死亡,则可以自动修复和恢复失去的功能,而无需药物干预治疗。再生医学是现代组织工程中使用的方法之一。研究人员已付出巨大努力,以设计和开发能够支持细胞的支架用于细胞疗法。然而,一些报道的水凝胶系统在交联后不易通过注射器注射,并且由于其不可移动的性质而不能为细胞提供合适的环境。根据已有报道,使用精心设计制备的可注射水凝胶系统不仅可以提供模仿天然人体ECM的环境,而且可以显著改善原位组织再生的程度。这项研究的主要内容和...
【文章来源】：中国科学技术大学安徽省 211工程院校 985工程院校
【文章页数】：81 页
【学位级别】：硕士
【文章目录】：
摘要
ABSTRACT
CHAPTER 1 GENERAL INTRODUCTION
1.1 Injectable hydrogels
1.2 Various methods of developing hydrogels
1.2.1 Crosslinking based on Michael addition reaction
1.2.2 Crosslinking based on irradiation
1.2.3 crosslinking based on Ionic interactions
1.2.4 In situ crosslinking based on Schiff base reaction
1.3 Natural and synthetic polymer-based hydrogels
1.4 Application of hydrogels
1.4.1 Hydrogels in wound healing and tissue repair
1.4.2 Hydrogels in tumor therapy
1.4.3 Cell support
1.5 Aim of the study
1.6 Summary of the thesis
REFERENCES
CHAPTER 2 DESIGN AND VIEWS ON HYDROGELS FOR CELL SUPPORT
2.1 Background
2.2 Natural biopolymers
2.2.1 Sodium alginate biopolymer
2.2.2 Chitosan
2.2.3 Hyaluronic acid
2.2.4 Gelatin
2.2.5 Chemical modification of natural biopolymers
2.2.6 Injectable hydrogels for protein delivery
2.2.7 Current studies on injectable hydrogels as therapeutic delivery systems
2.2.8 Injectable hydrogel for cell therapy
2.2.9 Injectable hydrogel formation through Schiff base reaction
REFERENCES
CHAPTER 3 SYNTHESIS OF OXIDIZED SODIUM ALGINATE BASEDINJECTABLE HYDROGELS
3.1 Structural units of alginate biopolymer
3.2 Reagents and instruments
3.3 Experimental procedures
3.3.1 Synthesis of aldehyde functionalized alginate (O.A)
3.3.2 Determination of oxidation degree
3.3.3 FTIR characterization of oxidized alginate
3.3.4 Quantification of amino groups of gelatin
3.3.5 Procedures for injectable hydrogel formation
3.3.6 Rheological measurement and gelation time
3.4 Procedures for cell experiment
3.4.1 Materials
3.4.2 Hydrogel biocompatibility test
3.5 Discussion of results
3.5.1 oxidation of native sodium alginate
3.5.2 Hydrogel formation
3.5.3 Rheological measurement and gelation time
3.5.4 Optimization of hydrogel formation condition
3.5.5 Hydrogel biocompatibility test
REFERENCES
CHAPTER 4 CONCLUSION AND FUTURE PERSPECTIVES
ACKNOWLDGEMENTS
PUBLISHED ACADEMIC PAPERS
本文编号：3382754
【文章来源】：中国科学技术大学安徽省 211工程院校 985工程院校
【文章页数】：81 页
【学位级别】：硕士
【文章目录】：
摘要
ABSTRACT
CHAPTER 1 GENERAL INTRODUCTION
1.1 Injectable hydrogels
1.2 Various methods of developing hydrogels
1.2.1 Crosslinking based on Michael addition reaction
1.2.2 Crosslinking based on irradiation
1.2.3 crosslinking based on Ionic interactions
1.2.4 In situ crosslinking based on Schiff base reaction
1.3 Natural and synthetic polymer-based hydrogels
1.4 Application of hydrogels
1.4.1 Hydrogels in wound healing and tissue repair
1.4.2 Hydrogels in tumor therapy
1.4.3 Cell support
1.5 Aim of the study
1.6 Summary of the thesis
REFERENCES
CHAPTER 2 DESIGN AND VIEWS ON HYDROGELS FOR CELL SUPPORT
2.1 Background
2.2 Natural biopolymers
2.2.1 Sodium alginate biopolymer
2.2.2 Chitosan
2.2.3 Hyaluronic acid
2.2.4 Gelatin
2.2.5 Chemical modification of natural biopolymers
2.2.6 Injectable hydrogels for protein delivery
2.2.7 Current studies on injectable hydrogels as therapeutic delivery systems
2.2.8 Injectable hydrogel for cell therapy
2.2.9 Injectable hydrogel formation through Schiff base reaction
REFERENCES
CHAPTER 3 SYNTHESIS OF OXIDIZED SODIUM ALGINATE BASEDINJECTABLE HYDROGELS
3.1 Structural units of alginate biopolymer
3.2 Reagents and instruments
3.3 Experimental procedures
3.3.1 Synthesis of aldehyde functionalized alginate (O.A)
3.3.2 Determination of oxidation degree
3.3.3 FTIR characterization of oxidized alginate
3.3.4 Quantification of amino groups of gelatin
3.3.5 Procedures for injectable hydrogel formation
3.3.6 Rheological measurement and gelation time
3.4 Procedures for cell experiment
3.4.1 Materials
3.4.2 Hydrogel biocompatibility test
3.5 Discussion of results
3.5.1 oxidation of native sodium alginate
3.5.2 Hydrogel formation
3.5.3 Rheological measurement and gelation time
3.5.4 Optimization of hydrogel formation condition
3.5.5 Hydrogel biocompatibility test
REFERENCES
CHAPTER 4 CONCLUSION AND FUTURE PERSPECTIVES
ACKNOWLDGEMENTS
PUBLISHED ACADEMIC PAPERS
本文编号：3382754
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