In vitro and In vivo Evaluation of the Developed PLGA/HAp/Ze

发布时间：2022-01-25 12:04

　　Objective To investigate the effect of electronspun PLGA/HAp/Zein scaffolds on the repair of cartilage defects. Methods The PLGA/HAp/Zein composite scaffolds were fabricated by electrospinning method. The physiochemical properties and biocompatibility of the scaffolds were separately characterized by scanning electron microscope（SEM）, transmission electron microscope（TEM）, and fourier transform infrared spectroscopy（FTIR）, human umbilical cord mesenchymal stem cells（h UC-MSCs） culture and animal... 

【文章来源】：Biomedical and Environmental Sciences. 2015,28(01)SCICSCD

【文章页数】：12 页

【文章目录】：
INTRODUCTION
MATERIALS AND METHODS
    Materials and Reagents
    Fabrication of PLGA/HAp/Zein Scaffolds
    Phyisochemical Characterization of PLGA/HAp/Zein Scaffolds
    Isolation and Identification of hUC-MSCs
    Culture of h UC-MSCs on PLGA/HAp/Zein Scaffolds
    Proliferation and Doubling Time of h UC-MSCs by MTT
    Cytotoxicity Test
    Preparation of Cell-scaffold Complexes
    Preparation and Grouping of Animal Model
    Morphological Observation
    Data Analysis
RESULTS
    Physiochemical Characterization of PLGA/HAp/Zein Scaffolds
    Morphological Observation and Determination of Surface Antigen Marker of h UC-MSCs
    Culture of h UC-MSCs on PLGA/HAp/Zein Scaffolds
    Cytotoxicity test of PLGA/HAp/Zein Scaffolds
    General Situation of the in vivo Experiment
    Gross Observation of the in vivo Experiment
    Histological Observation of the in vivo Experiment
DISCUSSION
CONCLUSION



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