WWP1在乳腺癌组织中的表达及意义

发布时间：2017-12-27 20:24

本文关键词：WWP1在乳腺癌组织中的表达及意义　出处：《南京医科大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的：探讨WW结构域E3泛连接酶1(WWP1)在乳腺癌病人组织中的表达,分析与乳腺癌病人临床病理因素的关系,并讨论WWPl基因和乳腺癌患者中ER、PR、P53基因、HER-2、年龄、淋巴结转移、病理组织学分级以及肿瘤组织大小之间的关系,为乳腺癌病人的临床治疗及预后提供科学依据。材料与方法：该实验选取2004年3月一2007年6月接受乳腺癌切除术的129例女性患者乳腺癌组织存档石蜡标本,应用组织芯片技术来构建乳腺癌病人组织芯片,通过HE染色和免疫组化染色来判断组织芯片质量以及观察WWP1表达情况；另选取乳腺良性病人手术切除的19例患者乳腺组织存档的石蜡标本玻片来作为对照组。从而将乳腺癌病人的年龄大小、肿瘤组织的大小、腋窝淋巴结侵袭转移、临床病理分期以及乳腺癌临床指标ER、PR和HER-2基因的表达情况与WWP1表达情况作相关分析,并得出结论。结果：HE玻片染色显示两组组织芯片染色合格,可以代表判断乳腺癌组织病理学形态。通过免疫组化染色观察得到,在乳腺癌病人组织中有92例(约70.5%)可见WWP1基因表达为阳性,乳腺良性组织中均未见WWP1表达。术后有腋窝淋巴结转移的病人WWP1阳性表达率明显高于WWP1阴性表达者(P0.05)；HER-2基因的表达和WWP1的表达呈正相关(P0.05)；肿瘤大小与WWP1阳性表达率呈正相关(P0.05)；乳腺癌组织的肿瘤分期和WWP1的阳性表达率之间无明显统计学意义(P0.05)。雌激素受体、孕激素受体表达与WWPl阳性表达率比较差异均无统计学意义(P0.05)。WWP1的表达和患者生存之间没有明显统计学意义(P0.05)。结论：WWP1和雌激素受体(ER)、孕激素受体(PR)、肿瘤病理分期之间无明显统计学意义,且WWP1和患者的预后生存之间无明显统计学意义；WWP1在乳腺癌组织中过表达且与肿瘤组织大小、淋巴结转移及Her-2基因的表达正相关,WWP1有望成为一个新的治疗靶点。
[Abstract]:Objective: To investigate the WW domain of E3 (WWP1) - ligase 1 expression in tissue of breast cancer patients in the relationship analysis and clinical pathological factors in breast cancer patients, and discuss the WWPl gene in patients with breast cancer and ER, PR, P53, HER-2 gene, age, lymph node metastasis, histological grade and the relationship between the size of the tumor tissue, and provide scientific basis for clinical treatment and prognosis of patients with breast cancer. Materials and methods: the experiment selected in March 2004 June 2007 to accept 129 female patients with breast cancer paraffin samples of resection of breast cancer, the application of tissue microarray technology to construct breast cancer patients by tissue chip, HE staining and immunohistochemical staining to determine the expression of chip quality and observation WWP1; the other from 19 cases of breast excision of benign breast tissues of patients with archived paraffin samples of patients as control group were. The correlation between the age of breast cancer, the size of tumor tissue, the invasion and metastasis of axillary lymph nodes, the clinicopathological staging, and the expression of ER, PR and HER-2 genes in breast cancer, and the expression of WWP1 were analyzed. Results: the staining of HE slides showed that two groups of tissue chips were coloring, which could represent the histopathological morphology of breast cancer. By immunohistochemical staining, 92 cases (70.5%) were found in breast cancer patients, and the expression of WWP1 gene was positive. There was no WWP1 expression in benign breast tissues. After the operation was significantly higher than the negative expression of WWP1 in patients with positive expression of WWP1 metastasis of axillary lymph node (P0.05); their expression is positively correlated with the expression of WWP1 and the HER-2 gene (P0.05) was positively correlated with tumor size; the positive expression rate of WWP1 (P0.05); breast cancer tissue and tumor stage between the positive expression rate of WWP1 no statistical significance (P0.05). There was no significant difference between the expression of estrogen receptor and progesterone receptor and the positive expression rate of WWPl (P0.05). There was no significant statistical significance between the expression of WWP1 and the survival of the patients (P0.05). Conclusion: WWP1 and estrogen receptor (ER) and progesterone receptor (PR), tumor pathological stage was not statistically significant, and prognosis in patients with WWP1 and no statistical significance; in the WWP1 over expression in breast cancer tissue and the expression and tumor size, lymph node metastasis and Her-2 gene positive correlation, WWP1 is expected to become a new therapeutic target.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R737.9

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