液相芯片技术检测胃癌血清细胞因子及临床意义

发布时间：2017-12-28 07:07

本文关键词：液相芯片技术检测胃癌血清细胞因子及临床意义　出处：《山西医科大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:探讨炎性相关细胞因子在胃癌病人血清中表达水平及各种细胞因子之间的相关性,并分析各个细胞因子与胃癌不同临床特征之间的关系。方法:运用高通量液相芯片技术对46例胃癌术前患者(胃癌组)和30例健康人(对照组)血清中16种因子IL-1β、IL-2、IL-4、IL-5、IL-6、IL-7、IL-8、IL-10、IL-12、IL-13、IL-17A、IL-21、IL-23、TNF-α、IFN-γ、GM-CSF表达水平进行同时检测。结果:1、胃癌组血清IL-8、IL-17A、IL-7、TNF-α表达水平较健康组显著升高,分别为[4.50(10.38)pg/ml vs.2.06(3.17)pg/ml,P=0.002;0.83(2.01)pg/ml vs.0.21(0.85)pg/ml,P=0.013;3.46(1.90)pg/ml vs.2.11(1.48)pg/ml,P=0.001;1.21(1.13)pg/ml vs.0.79(0.37)pg/ml,P0.001](P0.05);2、血清中IL-1β、IL-2、IL-4、IL-5、IL-6、IL-10、IL-12、IL-13、IL-21、IL-23、IFN-γ含量胃癌组均较健康组有升高趋势,但差异无统计学意义(P0.05);GM-CSF含量胃癌组较对照组降低,差异亦无统计学意义(P0.05);3、各细胞因子血清含量具有内在联系;4、胃癌累及淋巴结与未累及淋巴结比较差异无统计学意义,P均大于0.05。结论:血清中高水平的IL-7、IL-8、IL-17A、TNF-α可能参与胃癌的发生及发展,IL-8、IL-17A、TNF-α高表达可能是预后不良的指标,可能为临床诊断、病情监测、预后判断提供免疫学依据,抑制其表达可能作为治疗胃癌的方向之一。
[Abstract]:Objective: To investigate the correlation between inflammatory cytokines and the expression of cytokines in patients with gastric cancer, and analyze the relationship between cytokines and different clinical characteristics of gastric cancer. Methods: using high-throughput liquid chip technology in 46 cases of gastric cancer patients (gastric cancer group) and 30 healthy people (control group) 16 kinds of serum beta IL-2, IL-4, factor IL-1, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17A, IL-21, IL-23, TNF-, alpha IFN- y, GM-CSF expression levels were detected at the same time. Results: 1 gastric cancer, serum IL-8, IL-17A, IL-7, TNF- expression level was significantly higher than those in healthy group, respectively [4.50 (10.38) pg/ml vs.2.06 (3.17) pg/ml, P=0.002; 0.83 (2.01) pg/ml vs.0.21 (0.85) pg/ml, P=0.013; 3.46 (1.90) pg/ml vs.2.11 (1.48) pg/ml, P=0.001; 1.21 (1.13) pg/ml vs.0.79 (0.37) pg/ml, P0.001] (P0.05); 2, serum IL-1, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-21, IL-23, IFN- gamma content in gastric cancer group was significantly lower than that of healthy group increased, but the difference was not statistically significant (P0.05); GM-CSF the content of gastric carcinoma was lower than the control group, there was no significant difference (P0.05); 3, the serum cytokine content has the intrinsic relation; 4, lymph node involvement and gastric cancer without lymph node involvement had no significant difference, P was greater than 0.05. Conclusion: the occurrence and development of the high level of serum IL-7, IL-8, IL-17A and TNF- may be involved in gastric cancer, high expression of IL-8, IL-17A, TNF- alpha may be an indicator of poor prognosis, may provide immunological basis for clinical diagnosis and disease monitoring, prognosis, inhibition of its expression can be used as one of the direction of the treatment of gastric cancer.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.2

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