IL-17、IL-23在B细胞非霍奇金淋巴瘤外周血中的表达及意义

发布时间：2017-12-28 18:42

本文关键词：IL-17、IL-23在B细胞非霍奇金淋巴瘤外周血中的表达及意义　出处：《昆明医科大学》2016年硕士论文　论文类型：学位论文

【摘要】：[目的]本次课题通过检测B细胞非霍奇金淋巴瘤(non-Hodgkin lymphoma,NHL)患者外周血中IL-17、IL-23表达水平的变化,以探讨IL-17、IL-23在NHL发生发展过程中的作用。[方法]收集25名接受标准剂量RCHOP方治疗6-8个疗程后达到缓解的B细胞NHL患者作为NHL组,20名性别和年龄相匹配的健康者作为健康对照组。采集NHL组(包括每个患者初诊时及达到缓解时)和健康对照组所有受试对象的外周血。应用逆转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)法检测所有标本中IL-17和IL-23mRNA的表达水平,并收集NHL患者相关的临床资料。所有结果均利用统计软件SPSS17.0进行分析,以P0.05说明有统计学意义。[结果]1、NHL患者初诊时和缓解时外周血中IL-17mRNA的表达水平均低于健康对照组,差异均有统计学意义(P=0.000,P0.05),(P=0.OOO,P0.05)。而NHL患者缓解时与初诊时相比,IL-17mRNA的表达水平有所升高,差异也有明显统计学意义(P=0.001,P0.05)。2、NHL患者初诊时和缓解时外周血中IL-23mRNA的表达水平均低于健康对照组,差异均有统计学意义(P=0.000,P0.05),(P=0.000,P0.05)。而NHL患者缓解时与初诊时相比,IL-23mRNA的表达水平有所升高,差异也有明显统计学意义(P=0.004,P0.05)。3、NHL患者外周血中IL-17mRNA与IL-23mRNA的表达水平呈正相关关系(r=0.431,P=0.002)。4、NHL组患者外周血中IL-17、IL-23mRNA的水平与年龄、性别、全身症状、Ann Arbor分期、结外累及数目、乳酸脱氢酶、β2-微球蛋白及红细胞沉降率之间均无明显相关性(P0.05)。但国际预后指数(international prognosticindex,IPI)高的患者外周血中IL-17、IL-23mRNA的表达量较IPI低的患者明显降低,且差异有统计学意义(P0.05)。[结论]IL-17、IL-23可能参与了NHL发生、发展的病理过程,对判断患者疾病的严重程度、预后及疗效有一定意义,且二者在此过程中可能相互影响,具有协同作用。
[Abstract]:[Objective] to explore the role of IL-17 and IL-23 in the occurrence and development of NHL by detecting the changes of IL-17 and IL-23 expression levels in peripheral blood of B cell non-Hodgkin lymphoma (NHL) patients. [Objective] to explore the role of IL-17 and IL-23 in the occurrence and development of non-Hodgkin cells. [Methods] 25 B cell NHL patients who received the standard dose RCHOP prescription after 6-8 courses of treatment were collected as NHL group, and 20 healthy persons with gender and age matched as healthy control group. The peripheral blood of all subjects in the NHL group (including the first and remission of each patient) and the healthy control group were collected. The expression levels of IL-17 and IL-23mRNA in all specimens were detected by reverse transcription polymerase chain reaction (reverse transcription-polymerase chain reaction), and clinical data of NHL patients were collected. All the results were analyzed using the statistical software SPSS17.0, which was statistically significant in P0.05. [results the expression level of IL-17mRNA in peripheral blood of]1 and NHL patients was lower than that of healthy controls at the initial diagnosis and remission. The difference was statistically significant (P=0.000, P0.05), (P=0.OOO, P0.05). When compared with the first diagnosis, the expression level of IL-17mRNA increased in NHL patients, and the difference was statistically significant (P=0.001, P0.05). 2, the expression level of IL-23mRNA in peripheral blood of NHL patients was lower than that of healthy controls at the time of initial diagnosis and remission, and the difference was statistically significant (P=0.000, P0.05), (P=0.000, P0.05). When compared with the first diagnosis, the expression level of IL-23mRNA increased in NHL patients, and the difference was statistically significant (P=0.004, P0.05). 3, there was a positive correlation between the expression level of IL-17mRNA and IL-23mRNA in the peripheral blood of NHL patients (r=0.431, P=0.002). 4, the level of IL-17 and IL-23mRNA in peripheral blood of group NHL had no significant correlation with age, gender, systemic symptoms, Ann Arbor stage, number of extra nodal involvement, lactate dehydrogenase, beta 2- microglobulin and erythrocyte sedimentation rate (P0.05). However, the expression of IL-17 and IL-23mRNA in peripheral blood of patients with high international prognosticindex (IPI) was significantly lower than that of patients with low IPI, and the difference was statistically significant (P0.05). [conclusion]IL-17 and IL-23 may be involved in the pathological process of NHL occurrence and development. It has certain significance in judging the severity, prognosis and curative effect of patients, and the two may interact with each other in this process, which has synergistic effect.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R733.1

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