ZEB-1在胃癌组织中的表达及与胃癌生物学行为的关系

发布时间：2017-12-28 19:04

本文关键词：ZEB-1在胃癌组织中的表达及与胃癌生物学行为的关系　出处：《安徽医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的探讨胃癌组织中ZEB-1和c-jun蛋白的表达及对肿瘤发生、发展的影响。建立人胃癌细胞株BGC-823中ZEB-1基因沉默模型,研究其对胃癌细胞增殖、迁移、侵袭的影响及机制。方法采用免疫组化(IHC)法检测100例胃癌及癌旁组织中ZEB-1c-jun和E-cadherin的表达。构建沉默ZEB-1基因的si RNA干扰序列,转染到人胃癌细胞BGC-823,使用RT-PCR检测转染前后ZEB-1 m RNA表达的情况,挑选出沉默效率最高的si RNA干扰片段。使用CCK-8法检测细胞增殖情况,使用Transwell小室检测细胞的迁移和侵袭能力。结果胃癌组织ZEB-1、c-jun和E-cadherin的阳性表达率分别为81%、70%、35%,癌旁组织阳性表达率分别为17%、25%、100%。ZEB-1和c-jun蛋白在胃癌组织中的表达均显著高于癌旁组织(P0.05)。ZEB-1阳性表达与肿瘤浸润深度、分化程度、淋巴结转移、TNM分期有关(P0.05),而与年龄、性别、肿块大小无明显相关性。c-jun阳性表达与肿瘤分化程度有关(P0.05),与其它临床病理特征无明显相关。ZEB-1与E-cadherin在胃癌组织中表达存在负相关(P0.05),ZEB-1与c-jun表达存在正相关(P0.05)。ZEB-1与c-jun高表达患者术后5年生存率明显低于低表达患者。经RT-PCR检测,BGC-823细胞转染干扰si RNA后,ZEB-1-si RNA3表达在m RNA水平受到抑制最明显。CCK-8法检测细胞增殖能力,实验组、空白对照组、阴性对照组细胞的增殖能力无明显差异(P0.05)。细胞侵袭实验中,实验组细胞穿膜细胞数显著低于空白对照组和阴性对照组(P0.05)。细胞徖移实验中,实验组细胞穿膜细胞数显著低于空白对照组和阴性对照组(P0.05)。结论ZEB-1与c-jun在胃癌中的高表达与胃癌发生、发展密切相关,可作为判断胃癌预后的指标。ZEB-1在促进胃癌的侵袭转移过程中起到重要作用,可以成为控制胃癌浸润、转移的新靶点。
[Abstract]:Objective to investigate the expression of ZEB-1 and c-jun protein in gastric carcinoma and its effect on the occurrence and development of tumor. To establish the ZEB-1 gene silencing model in human gastric cancer cell line BGC-823, and to study the effect and mechanism of the model on the proliferation, migration and invasion of gastric cancer cells. Methods the expression of ZEB-1? C-jun and E-cadherin in 100 cases of gastric cancer and adjacent tissues were detected by immunohistochemistry (IHC). The Si RNA interference sequence of silencing ZEB-1 gene was constructed and transfected into human gastric cancer cell line BGC-823. The expression of ZEB-1 m RNA before and after transfection was detected by RT-PCR, and the Si RNA interference fragment with the highest silencing efficiency was selected. CCK-8 assay was used to detect cell proliferation, and Transwell cells were used to detect cell migration and invasiveness. Results the positive expression rates of ZEB-1, c-jun and E-cadherin in gastric cancer tissues were 81%, 70% and 35% respectively. The positive expression rates of para cancerous tissues were 17%, 25% and 100%, respectively. The expression of ZEB-1 and c-jun protein in gastric cancer tissues was significantly higher than that of para cancerous tissue (P0.05). The positive expression of ZEB-1 was related to tumor invasion depth, differentiation degree, lymph node metastasis and TNM stage (P0.05), but not related to age, sex and size of tumor. The positive expression of c-jun was associated with the degree of tumor differentiation (P0.05), and had no significant correlation with other clinicopathological features. There was a negative correlation between the expression of ZEB-1 and E-cadherin in gastric cancer tissues (P0.05), and there was a positive correlation between ZEB-1 and c-jun expression (P0.05). The 5 year survival rate of patients with high expression of ZEB-1 and c-jun was significantly lower than that of low expression patients. After RT-PCR detection, BGC-823 cells transfected with Si RNA, the expression of ZEB-1-si RNA3 was most inhibited at the level of M RNA. The proliferation of cells in the experimental group, the blank control group and the negative control group had no significant difference (P0.05) by CCK-8 assay. In the experiment of cell invasion, the number of cell transmembrane cells in the experimental group was significantly lower than that in the blank control group and the negative control group (P0.05). In the experimental group, the number of cell transmembrane cells in the experimental group was significantly lower than that in the blank control group and the negative control group (P0.05). Conclusion the high expression of ZEB-1 and c-jun in gastric cancer is closely related to the occurrence and development of gastric cancer, and can be used as an indicator to judge the prognosis of gastric cancer. ZEB-1 plays an important role in promoting the invasion and metastasis of gastric cancer, and can be a new target to control the invasion and metastasis of gastric cancer.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.2

【相似文献】