C35基因在结直肠癌中的表达及临床意义

发布时间：2018-01-01 01:09

本文关键词：C35基因在结直肠癌中的表达及临床意义　出处：《济南大学》2015年硕士论文　论文类型：学位论文

【摘要】：研究目的结直肠癌是常见的消化系恶性肿瘤之一,全球每年新发病例约800万人,占所有恶性肿瘤的10%～15%。随着生活条件及饮食习惯的改变,我国结直肠癌的发病率也逐年上升。结直肠癌的早期发现和治疗是至关重要的,这明显关系到癌症的预后。在早期,手术是能够治愈结直肠癌的重要手段,但发生转移后手术效果会明显下降,化疗可以提高生活质量和症状,而个性化的靶向药物治疗也是未来的发展趋势。所以识别结直肠癌中的特异性的标记物可以为治疗和预防提供重要的方法。研究方法收集山东省医学科学院第三附属医院2012年6月-2014年6月经内镜下或手术切除并确诊为结直肠癌标本共65例作为观察组,55例结直肠癌旁组织为对照组,其中50例癌旁组织与癌组织来源于同一本体,为下一步实时定量PCR做准备,10例正常组织来源于排除癌症患者。所有标本均放置RNA-free EP管中放置-80℃冰箱中保存备用。1、通过逆转录PCR的方法定性检测结直肠癌中C35基因的表达。2、采用Real-time PCR的方法检测结直肠癌组织与癌旁组织C35 mRNA水平表达的差异。收集金域检验机构2013年1月-2014年6月接收经手术切除、经病理诊断证实为结直肠癌的标本80例,正常组织10例。所有组织标本均记有病人的详细的基本信息,包括肿瘤的大小、Dukes分期、浸润程度、淋巴结转移等。1、采用免疫组织化学法检测结直肠癌组织及结直肠癌正常粘膜中C35蛋白的表达水平。2、分析C35表达与结直肠癌临床病理参数的关系。研究结果1、C35蛋白和mRNA在结直肠癌及癌旁组织中出现高表达,正常组织未见表达,在结直肠癌组织和癌旁组织中的相对表达量比较差异具有统计学学意义。2、C35染色的阳性物质成棕黄色,主要表达于胞质。C35在结直肠癌中的阳性表达高于正常粘膜中的表达,80例结直肠癌组织58例阳性表达,阳性表达率为72.5%,阴性为22例,正常组织中均为阴性表达。3、C35在结直肠癌中表达与患者的年龄、性别、肿瘤大小、肿瘤位置、分化程度都没有明显的相关性。而与淋巴结的转移、浆膜的浸润、远处转移都有显著的统计学意义。研究结论C35无论是在mRNA转录水平还是在蛋白质水平均在结直肠癌中表现出较高的表达,并且是结直肠癌发生、发展过程中一个重要的作用因子,C35激活及促进肿瘤的发展在一定的方面已经得到证实,是监测结直肠癌发生的一个新的重要靶点。如果能进一步了解C35的结构功能,设计合理的药物用于临床等方面,C35有可能成为结直肠癌基因治疗的一个新靶点。
[Abstract]:Objective colorectal cancer is one of common malignant tumors in digestive system, around the world each year about 8 million new cases, accounting for 10% of all malignant tumors to 15%. with the living conditions and changes in diet, the incidence rate of colorectal cancer in China has increased year by year. Colorectal cancer early detection and treatment is very important, this is obvious related to the prognosis of cancer. In the early stage, surgery is an important means to cure colorectal cancer metastasis after surgery, but the effect will be significantly decreased, chemotherapy can improve life quality and symptoms, and individualized targeted therapy is the future trend of development. Therefore the recognition node specificmarker rectal carcinoma can provide an important the methods for prevention and treatment. Methods from June 2012 Third Affiliated Hospital of Shandong Academy of Medical Sciences -2014 6 menstrual endoscopic or surgical resection and diagnosed with colorectal intestinal Cancer samples were 65 cases as the observation group, 55 cases of colorectal cancer tissues as the control group, including 50 cases of carcinoma adjacent tissues and cancer tissues from the same body, prepare for the next step of real-time quantitative PCR, 10 cases of normal tissue from the exclusion of cancer patients. All specimens were placed EP tube placed in -80 RNA-free C the refrigerator spare.1,.2 expression by reverse transcription PCR method for qualitative detection of colorectal cancer C35 gene, differences in colorectal cancer tissues and paracancerous tissue C35 mRNA expression by Real-time PCR method. Collected the Golden Mile inspection institutions in January 2013 -2014 year in June after receiving surgical resection, confirmed by pathological diagnosis for colorectal cancer samples of 80 cases, 10 cases of normal tissue. All the specimens were recorded with the patient's basic information, including tumor size, Dukes stage, infiltration degree and lymph node metastasis of.1 by immunohistochemical method. Detection of C35 protein in rectal cancer tissue and normal mucosa of colorectal cancer the expression level of.2, analysis of the relationship between C35 expression and clinicopathological features of colorectal cancer. Results: 1, the high expression of C35 protein and mRNA in colorectal carcinoma and adjacent tissues, the expression of normal tissues, the relative expression in colorectal cancer tissues and para carcinoma the amount of the difference was statistically significant.2 positive substance C35 stained brown, mainly expressed in the cytoplasm of positive expression of.C35 in colorectal carcinoma was higher than that in normal mucosa, the positive expression in 58 cases of 80 cases of colorectal cancer, the positive expression rate was 72.5%, negative in 22 cases, normal tissues. The expression of.3 was negative, C35 expression and patient age, gender, tumor size, tumor location, degree of differentiation in colorectal cancer, there is no obvious correlation with lymph node metastasis. The plasma membrane, invasion, distant Have statistical significance. Conclusion C35 transfer both in the transcription level of mRNA or protein levels in colorectal cancer showed higher expression and colorectal cancer, an important factor in the development process, the activation of C35 and promote tumor development in some aspects has been confirmed, is a new and important target for monitoring colorectal cancer. If we can further understand the structure and function of C35, the rational design of drugs for clinical aspects, C35 may become a new target of gene therapy for colorectal cancer.

【学位授予单位】：济南大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.34

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