ULK1和ANGPTL3在食管鳞状细胞癌组织中的表达及意义

发布时间：2018-01-01 15:07

本文关键词：ULK1和ANGPTL3在食管鳞状细胞癌组织中的表达及意义　出处：《扬州大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的：检测ULKl和ANGPTL3在食管鳞状细胞癌组织中的表达及其相关性,探讨两者在食管鳞状细胞癌(ESCC)发生、发展中的作用及其临床预后判断价值。方法：应用组织微阵列技术结合免疫组织化学染色方法(EnVision两步法)检测86例食管鳞状细胞癌组织以及78例癌旁组织中ULK1和ANGPTL3蛋白的表达情况,并分析两者与临床病理参数和生存时间之间的相关性。结果：1、在食管鳞状细胞癌组织和癌旁组织中,ULK1蛋白表达阳性率分别为61.6%和23.1%,差异具有统计学意义(x2=24.761,p=0.000)；ANGPTL3蛋白表达阳性率分别为59.3%和14.1%,差异具有统计学意义(x2=35.540,p=0.000)。ULKl蛋白表达水平与TNM分期(x2=6.320,p=0.012)具有显著的相关性。ANGPTL3蛋白表达水平与性别、年龄、肿瘤大小、组织学分级、TNM分期、淋巴结转移(LNM)之间均没有明显的相关性(P0.05)。2、Spearman秩相关分析显示,ULK1蛋白表达与ANGPTL3蛋白表达呈正相关(r=0.222,p=0.040)。3、应用Kaplan-Meier生存分析和COX单因素分析表明,TNM分期Ⅲ+Ⅳ(危险比=1.779,95%置信区间：1.035-3.058,P=0.037)、淋巴结转移(危险比=1.765,95%置信区间：1.026-3.038,P=0.040)、ULK1胞浆阴性表达(危险比=0.508,95%置信区间：0.296-0.873,P=0.014)、ANGPTL3胞浆阳性表达(危险比=1.880,95%置信区间：1.043-3.388,P=0.036)与食管鳞状细胞癌患者较差的预后显著相关。COX多因素分析表明,ULK1蛋白表达水平(危险比=0.471,95%置信区间：0.265-0.836,P=0.010)、ANGPTL3蛋白表达水平(危险比=2.156,95%置信区间：1.177-3.950,P=0.013)是食管鳞状细胞癌患者的独立预后因素。结论：1、ULK1和ANGPTL3在食管鳞状细胞癌组织中的阳性表达率明显高于癌旁组织,且两者的表达呈正相关。提示ULK1和ANGPTL3可能参与了食管鳞状细胞癌的发生、发展过程。2、ULK1蛋白表达水平与TNM分期(x2=6.320,p=0.012)具有显著的相关性。提示ULK1的表达可能与食管鳞状细胞癌患者恶性生物学行为相关。3、ULK1和ANGPTL3与临床预后有一定的相关性,两者有可能成为食管鳞状细胞癌患者预后判断的分子标志物。
[Abstract]:Objective: To study the expression and correlation between ULKl and ANGPTL3 in esophageal squamous cell carcinoma, to explore both in esophageal squamous cell carcinoma (ESCC), its clinical prognostic value. Methods: by using tissue microarray and immunohistochemistry (EnVision two steps) the expression of ULK1 and ANGPTL3 protein detection 86 cases of esophageal squamous cell carcinoma and 78 cases of adjacent tissues, and to analyze the correlation between clinical and pathological parameters and survival time. Results: 1, the organization in esophageal squamous cell carcinoma and the expression of ULK1 protein, the positive rates were 61.6% and 23.1%, the difference was statistically significant (x2=24.761. P=0.000); the positive rate of ANGPTL3 expression were 59.3% and 14.1%, the difference was statistically significant (x2=35.540, p=0.000).ULKl expression and TNM staging (x2=6.320, p=0. 012) there was significant correlation between.ANGPTL3 expression and gender, age, tumor size, histological grade, TNM staging, lymph node metastasis (LNM) have no obvious correlation between.2 (P0.05), Spearman rank correlation analysis showed that ANGPTL3 was positively correlated with ULK1 expression (r=0.222,.3, p=0.040) application of Kaplan-Meier univariate survival analysis and COX analysis showed that TNM stage III + IV (hazard ratio =1.779,95% confidence interval: 1.035-3.058, P=0.037), lymph node metastasis (hazard ratio =1.765,95% confidence interval: 1.026-3.038, P=0.040), the expression of cytoplasmic ULK1 negative (hazard ratio =0.508,95% confidence interval: 0.296-0.873, P=0.014, ANGPTL3) positive cytoplasm the expression (hazard ratio =1.880,95% confidence interval: 1.043-3.388, P=0.036) and poor prognosis in patients with esophageal squamous cell carcinoma was significantly related to.COX multivariate analysis showed that the expression level of ULK1 protein (hazard ratio =0 .471,95% confidence interval: 0.265-0.836, P=0.010), the expression level of ANGPTL3 protein (hazard ratio =2.156,95% confidence interval: 1.177-3.950, P=0.013) is an independent prognostic factor in patients with esophageal squamous cell carcinoma. Conclusion: 1. ULK1 and ANGPTL3 positive in esophageal squamous cell carcinoma tissues was significantly higher than that in paracancerous tissues, and their expression related. Suggesting that ULK1 and ANGPTL3 may be involved in the carcinogenesis of esophageal squamous cell carcinoma, the development process of.2, the expression level of ULK1 protein and TNM stage (x2=6.320, p=0.012). There was significant correlation between the expression of ULK1 that may be associated with esophageal squamous cell carcinoma patients with malignant biological behavior of.3, ULK1 and ANGPTL3 have a certain correlation with the clinical prognosis, both may become a molecular marker for predicting prognosis of patients with esophageal squamous cell carcinoma.

【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.1

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