ERG与TERT启动子的相关研究

发布时间：2018-01-03 20:00

本文关键词：ERG与TERT启动子的相关研究　出处：《杭州师范大学》2016年硕士论文　论文类型：学位论文

【摘要】：端粒酶可以维持端粒长度,从而有效的防止细胞衰老的发生,但是端粒酶活性的分布却不均匀,主要体现在干细胞中,在分化的细胞中,端粒酶活性一般被抑制,而端粒酶在85%的癌细胞中却处在激活状态。最近,人们发现ETS转录因子可以调控端粒酶活性,其中ERG在调控hTERT与癌症发生中发挥着巨大作用。更有趣的是人们发现人癌细胞大量存在C228T和C250T这两种Tert启动子的突变体,这为我们去了解端粒酶的重激活提供了新方向。在本文的研究中,我们将着重关注干扰ERG后,TERT突变启动子的活性,细胞衰老和增殖能力的变化,同时去了解ERG与USP9X的互作情况。从最后的结果来看,我们更加肯定了ERG表达变化对肿瘤细胞的影响。
[Abstract]:Telomerase can maintain telomere length, thus effectively prevent the occurrence of cell senescence, but the distribution of telomerase activity is uneven, mainly in stem cells, in differentiated cells. Telomerase activity is generally inhibited, while telomerase activity is activated in 85% of cancer cells. Recently, it has been found that ETS transcription factors regulate telomerase activity. ERG plays an important role in the regulation of hTERT and carcinogenesis. More interesting is the discovery of a large number of C228T and C250T Tert promoter mutants. This provides a new direction for us to understand the reactivation of telomerase. In this study, we will focus on the changes in the activity, senescence and proliferation of ERG mutant promoter. At the same time, to understand the interaction between ERG and USP9X. From the final results, we confirm the effect of ERG expression on tumor cells.
【学位授予单位】：杭州师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R730.2

【相似文献】