EGFR、PI3K、PTEN在人脑胶质瘤中的表达及临床意义分析

发布时间：2018-01-04 01:18

  本文关键词：EGFR、PI3K、PTEN在人脑胶质瘤中的表达及临床意义分析　出处：《昆明医科大学》2016年硕士论文　论文类型：学位论文

  更多相关文章： 脑胶质瘤 EGFR PI3K PTEN

【摘要】：[目的]本研究检测PI3K信号通路相关蛋白EGFR、PI3K和PTEN在人脑胶质瘤中的表达情况。分析其表达与肿瘤级别、患者临床指标的关系,以及这几种蛋白之间的相关性,探讨其临床意义,以期对人脑胶质瘤靶向治疗的临床应用提供参考。[方法]收集昆明医科大学第一附属医院2014年1月至2015年6月符合入选条件的人脑胶质瘤石蜡标本50例。采用免疫组化SP法分别检测EGFR、PI3K和PTEN蛋白在50例标本中的表达,记录染色强度及阳性细胞率。采用χ2检验比较不同级别胶质瘤,不同性别、年龄及肿瘤大小中各蛋白的表达差异。采用Spearman等级相关分析检验EGFR,PI3K及PTEN之间相关性。[结果]在本研究50例脑胶质瘤标本中,EGFR蛋白总的阳性率为50%,在低级别胶质瘤中阳性率为34.62%,在高级别胶质瘤中阳性率为66.67%,差异有统计学意义(P0.05)。PI3K蛋白总的阳性率为60%,在低级别胶质瘤中阳性率为46.15%,在高级别胶质瘤中阳性率为75%,差异有统计学意义(P0.05)。PTEN蛋白总的阳性率为54%,在低级别胶质瘤中阳性率为69.23%,在高级别胶质瘤中阳性率为37.5%,差异有统计学意义(P0.05)。EGFR、PI3K和PTEN在不同性别、不同年龄段及不同肿瘤大小中的表达无统计学差异(P0.05)。EGFR的表达与PI3K的表达呈正相关(r=0.327,P0.05),PTEN的表达与EGFR,PI3K的表达均呈负相关(r=-0.281,r=-0.344,P0.05)。[结论]1.EGFR,PI3K的表达水平随胶质瘤级别上升而增高,PTEN的表达水平随胶质瘤级别上升而下降。2.EGFR、PI3K和PTEN的表达与患者性别、年龄(50岁或≥50岁)及肿瘤大小(5cm或≥5cm)无关。3.EGFR的表达与PI3K的表达呈正相关,EGFR过表达可能通过激活PI3K信号通路在胶质瘤的发生发展中起作用。4.PETN的表达与EGFR、PI3K的表达呈负相关,揭示在脑胶质瘤中PTEN可能与PI3K信号通路拮抗,PTEN表达下降或缺失与EGFR过表达共同作用于PI3K信号通路,促进胶质瘤的发生及恶性转化。
[Abstract]:[Objective] to detect the expression of PI3K signaling pathway related proteins EGFRRP-PI3K and PTEN in human glioma and to analyze the relationship between the expression of EGFRP-PI3K and tumor grade and clinical index of patients. To explore the clinical significance of these proteins in order to provide reference for the clinical application of targeted therapy for human glioma. [Methods A total of 50 paraffin specimens of human glioma were collected from the first affiliated Hospital of Kunming Medical University from January 2014 to June 2015. EGFR were detected by immunohistochemical SP method. The expression of PI3K and PTEN protein in 50 samples were recorded. The staining intensity and the positive cell rate were recorded. 蠂 2 test was used to compare different grades of gliomas, different genders. Spearman grade correlation analysis was used to test the correlation between EGFR PI3K and PTEN. [Results: the total positive rate of EGFR protein was 50 in 50 gliomas and 34.62% in low grade gliomas. In high grade glioma, the positive rate was 66.67, the difference was statistically significant. The total positive rate of P0.05U. PI3K protein was 60 in high grade glioma, and the positive rate in low grade glioma was 46.15%. In high grade glioma, the positive rate was 75. The total positive rate of P0.05. PTEN protein was 54 and the positive rate of low grade glioma was 69.23%. The positive rate in high grade glioma was 37.5%, the difference was statistically significant (P 0.05). EGFRN PI3K and PTEN were different in sex. There was no significant difference in the expression of P0.05P. EGFR and the expression of PI3K in different age groups and different tumor sizes. There was a positive correlation between the expression of P0.05 and the expression of PI3K. There was a negative correlation between the expression of PTEN and EGFR PI3K. [Conclusions: 1. The expression of PI3K increased with the increase of glioma grade. The expression level of PTEN decreased with the increase of glioma grade. 2. The expression of PI3K and PTEN was not correlated with sex, age 50 years old or 鈮，

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