磷酸丙糖异构酶在人肺腺癌组织与血清中的表达对比研究

发布时间：2018-01-05 17:24

本文关键词：磷酸丙糖异构酶在人肺腺癌组织与血清中的表达对比研究　出处：《南华大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:研究论证磷酸丙糖异构酶(triose phosphate isomerase TPI)在人肺腺癌组织与配对的癌旁正常肺组织中的差异表达,以及在术前血清和术后血清样本中的差异表达,并探讨该酶在血清中与组织中的表达程度有无相关性,即是否可以用血清中检测代替组织中的检测,从而更方便有效地为肺腺癌的诊断和预后做出评价。方法:(1)取44例新鲜人肺腺癌组织及其配对的癌旁正常肺组织来自湖南省肿瘤医院胸外一科的未经放疗、化疗的肺癌手术切除的肺叶标本,并经湖南省肿瘤医院病理诊断确诊为肺腺癌(不含其它组织癌变成分);(2)取24例肺良性疾病患者对照组,标本来源为湖南省肿瘤医院胸外一科手术切除的肺组织,包括支气管肺炎、炎性假瘤、肺结核等,所有肺良性疾病患者均无肿瘤病史;(3)抽取上述44例人肺腺癌患者以及24例肺良性疾病患者术前1天的血液标本10ml及术后第7天的血液标本10ml。统计各组TPI总体均数并进行统计学分析,血清ELISA方法采用Wilcoxon秩和检验,免疫组化结果采用秩和检验。结果:ELISA结果分析:(1)术前肺部良性病变组(A1)与术后肺部良性病变组(A2)相比较,使用Wilcoxon秩和检验,Z=0.517,双侧检验P=0.605,P0.05,无统计学意义;(2)术前肺腺癌组(B1)与术后肺腺癌组(B2)相比较,使用Wilcoxon秩和检验,Z=1.228,双侧检验P=0.219,P0.05,无统计学意义;(3)肺良性病变组与肺腺癌组的下降值相比较,使用Wilcoxon秩和检验,Mann-Whitney U=430;Wilcoxon W=706;Z=0.270,双侧检验P=0.787,P0.05,无统计学意义;(4)两者组间相比较,即A1与B1相比较,使用Wilcoxon秩和检验,Z=0.274,双侧检验P=0.784,P0.05,无统计学意义。免疫组化统计结果分析:(1)磷酸丙糖异构酶在良性病变组织、肺腺癌组织和与其配对的正常肺组织表达有显著差异性,P0.01;(2)磷酸丙糖异构酶在配对的正常肺组织和肺良性病变组织之间没有统计学意义,P0.05;(3)磷酸丙糖异构酶的表达在配对的正常肺组织和肺腺癌组织中有差异统计学意义,且P0.01;(4)磷酸丙糖异构酶的表达在肺良性病变组织和肺腺癌组织中有差异统计学意义,P0.01。结论:(1)术前的肺良性病变组与肺腺癌组的血清TPI浓度值不具有差异性;(2)肺良性病变组中的术前与术后的血清TPI浓度值无明显变化;(3)肺腺癌组中术后较术前的血清TPI浓度值有所下降,但无统计学意义;(4)手术治疗方式对术前术后的血清TPI浓度的变化在肺良性病变组与肺腺癌组中无明显差异。(5)肺腺癌癌旁正常肺组织与肺部良性病变组织中磷酸丙糖异构酶的表达无明显统计学差异,即磷酸丙糖异构酶在腺癌中高表达,在正常肺组织及肺部良性病变组织中不表达或弱阳性表达,提示磷酸丙糖异构酶是肺腺癌的差异表达的蛋白质。(6)因为磷酸丙糖异构酶在肺腺癌患者与肺良性病变患者的血清中无差异表达,故此酶用于肺腺癌的血液检测临床检验意义不大。
[Abstract]:Objective: To study the triose phosphate isomerase (triose phosphate isomerase TPI) expression in human lung cancer tissues and adjacent normal lung tissues, and the differences in serum of preoperative and postoperative serum samples in the expression, and to explore the extent of expression of the enzyme in the serum and tissues had no correlation that is, whether can check instead of tissues detected by serum, and thus a more convenient and effective for the diagnosis and prognosis of lung adenocarcinoma was evaluated. Methods: (1) 44 cases of fresh human lung adenocarcinoma tissues and adjacent normal lung tissue from the tumor hospital of Hunan Province, without a chest radiotherapy, chemotherapy of lung cancer resection surgery lobectomy were diagnosed by pathology and Tumor Hospital of Hunan province for lung cancer (excluding other cancer tissue into points); (2) from 24 patients with benign lung disease group were from Thoracic Tumor Hospital of Hunan Province A surgical resection of the lung tissue, including pneumonia, inflammatory pseudotumor, pulmonary tuberculosis, all benign lung disease patients had no history of cancer; (3) from the 44 cases of patients with lung adenocarcinoma and 24 cases of blood samples and seventh days 10ml days before operation in 1 patients with benign lung diseases after operation the blood samples of 10ml. TPI were statistically analyzed the overall mean and statistical analysis, serum ELISA method using Wilcoxon rank sum test, the results of immunohistochemistry and Wilcoxon test. Results: the results of ELISA analysis: (1) preoperative benign lung disease group (A1) and postoperative pulmonary benign lesions group (A2) compared and using the Wilcoxon test, Z=0.517 test, P0.05, bilateral P=0.605, no statistical significance; (2) preoperative pulmonary adenocarcinoma group (B1) and postoperative pulmonary adenocarcinoma group (B2) compared with Wilcoxon rank test, Z=1.228 test, P0.05, bilateral P=0.219, no statistical significance (3; benign lung disease group) and lung adenocarcinoma Decline in cancer group compared with the values, using the Wilcoxon test, Mann-Whitney U=430; Wilcoxon W=706; Z=0.270, P=0.787 P0.05, two-sided test, no statistical significance; (4) the two groups compared to that of A1. Compared with B1, using the Wilcoxon test, Z=0.274 test, double side P=0.784, P0.05, no statistical significance. Immunohistochemical analysis of the statistical results: (1) the triose phosphate isomerase in benign tissues, lung adenocarcinoma tissues and matched normal lung tissue expression had significant differences, P0.01; (2) the triose phosphate isomerase was not statistically significant between the paired normal lung tissue and lung benign lesion tissues P0.05; (3) the expression of triosephosphate isomerase have statistically significant difference, in normal lung tissue and paired lung cancer tissues and P0.01; (4) the expression of triosephosphate isomerase has statistically significant difference in benign lung lesions and lung cancer tissues Conclusion: P0.01. (1), benign lung disease group and lung cancer group, the serum TPI concentration of preoperative value does not have differences; (2) benign lung disease group in the preoperative and postoperative serum TPI concentration was not significantly different; (3) lung cancer group serum TPI concentration of after compared with the preoperative values have declined, but without statistical significance; (4) surgical treatment changes of serum concentration of TPI before and after surgery had no obvious difference in benign lung disease group and lung cancer group. (5) the expression of triosephosphate isomerase in lung adenocarcinoma and adjacent normal lung tissue pulmonary benign lesions was no significant difference, i.e. triosephosphate isomerase in adenocarcinoma with high expression, no expression or weak expression in normal lung tissue and lung benign lesion tissues, suggesting that triose phosphate isomerase is differentially expressed in lung adenocarcinoma protein. (6) because of the triose phosphate isomerase in patients with lung adenocarcinoma and lung benign There is no differential expression in the serum of the patients with sexual lesions, so the clinical test of the enzyme used in the detection of lung adenocarcinoma is of little significance.

【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R734.2

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