多西紫杉醇对肝癌细胞裸鼠移植瘤抑制效应的研究

发布时间：2018-01-10 10:18

本文关键词：多西紫杉醇对肝癌细胞裸鼠移植瘤抑制效应的研究　出处：《昆明医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：[目的]探讨多西紫杉醇对肝癌细胞株裸鼠皮下移植瘤生长的影响及其可能的机制。[方法]建立肝癌细胞株SMMC-7721裸鼠皮下移植瘤模型,建模成功后,将20只裸鼠分成两组,多西紫杉醇组及肿瘤对照组各10只。实验组用多西紫杉醇处理,每只50ug · 0.3ml-1,每3天注射1次,连续注射7次。对照组注射生理盐水,剂量同前。连续观察两组裸鼠在整个实验期间的体重、移植瘤体积及一般情况的变化。3周后处死动物,称重及测量体积。切取瘤样组织,用TUNEL法检测肿瘤细胞凋亡情况,免疫组化法检测hTERT、Ki67、VEGF及P53阳性表达率。[结果]实验组较对照组在多西紫杉醇处理后,肿瘤生长速率明显减慢,肿瘤体积和瘤重明显也低于对照组,两者间差异有统计学意义(P0.05)；实验组在给药前后的体重无明显差异(p0.05)。实验组的肿瘤细胞凋亡指数高于对照组,两者间差异有统计学意义(P0.05)。实验组hTERT、Ki67、VEGF及P53阳性表达率均低于对照组,差异均有统计学意义(P0.05)。[结论]多西紫杉醇对肝细胞癌裸鼠移植瘤具有生长抑制作用,诱导肝癌细胞凋亡与抑制肝癌细胞增殖的作用机制可能与降低hTERT、P53、Ki-67蛋白的表达有关,阻断血管内皮细胞的迁移可能与降低VEGF蛋白的表达有关。多西紫杉醇在治疗浓度范围内对裸鼠无明显的毒性作用。
[Abstract]:[Objective] to investigate the effect of docetaxel on the growth of hepatoma cell line in nude mice and its possible mechanism. [Methods] the subcutaneous transplanted tumor model of SMMC-7721 nude mice was established and 20 nude mice were divided into two groups. The experimental group was treated with docetaxel 50 ug 路0.3 ml-1 and injected once every 3 days. The rats in the control group were injected with normal saline at the same dose as before. The body weight, the volume of the transplanted tumor and the general condition of the nude mice in the two groups were observed continuously during the whole experiment period. 3 weeks later, the animals were killed. The tumor cell apoptosis was detected by TUNEL method, and the expression rate of VEGF and p53 was detected by immunohistochemistry. [Results: after treated with docetaxel, the tumor growth rate, tumor volume and tumor weight in the experimental group were significantly lower than those in the control group, and the difference between the two groups was statistically significant (P 0.05). There was no significant difference in body weight before and after administration in the experimental group (p0.05). The apoptotic index of tumor cells in the experimental group was higher than that in the control group, and the difference between the two groups was statistically significant (P 0.05). HTERT in the experimental group. The positive expression rates of VEGF and p53 in Ki67 were significantly lower than those in the control group (P 0.05). [Conclusion: docetaxel can inhibit the growth of hepatocellular carcinoma xenografts in nude mice. The mechanism of inducing apoptosis and inhibiting the proliferation of hepatoma cells may be related to the reduction of hTERT p53. The inhibition of vascular endothelial cell migration may be related to the decrease of VEGF protein expression. Docetaxel has no obvious toxic effect on nude mice in the range of therapeutic concentration.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.7

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