IGF2BP2过表达与胶质瘤细胞周期及侵袭性的相关性研究

发布时间：2018-01-11 02:32

本文关键词：IGF2BP2过表达与胶质瘤细胞周期及侵袭性的相关性研究　出处：《宁夏医科大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的探讨胰岛素样生长因子2结合蛋白2(IGF2BP2)过表达与胶质瘤细胞周期及侵袭性的相关性。方法构建GV146-IGF2BP2真核表达载体,并利用实时荧光定量PCR技术和Western Blot技术检测过表达组、阴性对照组及空白对照组细胞中IGF2BP2基因在m RNA和蛋白水平的表达情况,以确定载体是否构建成功;流式细胞仪检测各组细胞的周期变化情况;Transwell技术检测各组细胞的侵袭性变化情况。结果(1)实时荧光定量PCR检测结果:过表达组细胞IGF2BP2的m RNA相对表达量与阴性对照组和空白对照组之间有显著差异,且阴性对照组和空白对照组之间差异无统计学意义;(2)蛋白印迹检测结果:过表达组细胞IGF2BP2的蛋白表达量与阴性对照组和空白对照组之间有显著差异,且阴性对照组和空白对照组之间差异无统计学意义;(3)流式细胞仪检测细胞周期结果:过表达组细胞处于G0/G1期的细胞数与阴性对照组和空白对照组之间有显著差异,且阴性对照组和空白对照之间差异无统计学意义;(4)Transwell实验结果:过表达组细胞穿过小室的个数与阴性对照组和空白对照组之间有显著差异,且阴性对照组和空白对照组之间差异无统计学意义。结论(1)GV146-IGF2BP2真核表达载体构建成功;(2)IGF2BP2过表达能够将细胞周期阻滞于G0/G1期,从而抑制肿瘤细胞的增殖;(3)IGF2BP2过表达可以使胶质瘤细胞的侵袭性减弱。
[Abstract]:Objective to investigate the effects of insulin-like growth factor binding protein 2 (IGF2BP2 2) expression and glioma cell cycle and invasion of correlation. Methods the eukaryotic expression vector GV146-IGF2BP2 was constructed, and using the real-time fluorescence quantitative PCR technology and Western Blot technology to detect over expression of control group, the expression of IGF2BP2 gene in M cells and RNA protein levels the negative control group and blank, to determine whether the carrier is successfully constructed; periodic changes of cells were detected by flow cytometry; invasive changes were assayed by Transwell technique. Results (1) real time fluorescence quantitative PCR test results: the M Expression of RNA cells the relative expression of IGF2BP2 and negative control there is a significant difference between the group and the control group, and the differences between the negative control group and blank control group was not statistically significant; (2) detection results of Western blot: cells of IGF2BP2 The amount of protein expression and there was significant difference between negative control group and blank control group, and the differences between the negative control group and blank control group was not statistically significant; (3) flow cytometry results: the cells in G0/G1 phase cells and negative control group were significant differences between groups and blank control group and negative control group and blank control between the difference was not statistically significant; (4) the results of Transwell: cells through cell number and there is significant difference between negative control group and blank control group, and the difference between negative control group and blank control group was not statistically significant. Conclusion (1) GV146-IGF2BP2 the successful construction of eukaryotic expression vector; (2) the overexpression of IGF2BP2 can block cell cycle in G0/G1 phase, thus inhibiting the proliferation of tumor cells; (3) the overexpression of IGF2BP2 can make the invasiveness of glioma cells decreased.

【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R739.41

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