前列腺癌临床患病因素及相关蛋白表达的研究

发布时间：2018-01-11 22:20

本文关键词：前列腺癌临床患病因素及相关蛋白表达的研究　出处：《宁夏医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：第一部分宁夏回族自治区的回族及汉族前列腺癌患病因素的病例对照研究目的探究宁夏回族自治区的回族、汉族人群的前列腺癌患者临床患病因素与前列腺癌的相关性。方法收集2007年1月至2013年9月在宁夏医科大学总医院住院并确诊为前列腺癌患者267例作为病例组,包括汉族214例,回族53例;良性前列腺增生患者534例作为对照组,包括汉族428例,回族106例。通过查找病例记录收集前列腺癌患者的空腹血糖(fasting blood glucose,FBG)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、总前列腺特异抗原(total prostate specific antigen,t-PSA)、游离前列腺特异抗原(free prostate specific antigen,f-PSA)、游离与总前列腺特异抗原比值(f-PSA/t-PSA,f/t-PSA),采用多因素条件logistic回归模型对前列腺癌的危险因素进行分析。结果回族饮酒者患前列腺癌的风险是非饮酒者的20.48倍[35.8%(19/53)比5.7%(6/106),OR=20.48,95%CI:4.95~84.66];高f-PSA水平增加回族人群前列腺癌的发病风险[83.0%(44/53)比55.7%(59/106),OR=4.27,95%CI:1.18~15.43];而高TG水平降低回族人群的前列腺癌发病风险[18.9%(10/53)比20.8%(22/106),OR=0.24,95%CI:0.07~0.83]。汉族人群中吸烟者患前列腺癌的风险是汉族非吸烟者的1.89倍[55.1%(118/214)比39.7%(170/428),OR=1.89,95%CI:1.28~2.78];高t-PSA水平[86.4%(185/214)比53.7%(230/428),OR=2.34,95%CI:1.22~4.52]和高f-PSA水平[85.5%(183/214)比56.1%(240/428),OR=2.43,95%CI:1.29~4.59]分别都会增加汉族人群的前列腺癌发病风险;而高TG水平[15.4%(33/214)比18.7%(80/428),OR=0.59,95%CI:0.36~0.98]和高f/t-PSA比值[53.3%(114/214)比73.4%(314/428),OR=0.53,95%CI:0.36~0.78]降低汉族人群的前列腺癌发病风险。结论饮酒和高f-PSA水平是回族人群前列腺癌发病的危险因素;高TG是回族前列腺癌发病的保护因素。吸烟、高t-PSA和高f-PSA水平是汉族前列腺癌的危险因素;高TG水平和高f/t-PSA比值为汉族前列腺癌的保护因素。宁夏地区回族、汉族人群前列腺癌发病风险的影响因素之间存在差异。第二部分ERG和MDM2在前列腺癌组织中表达的相关性研究目的检测ERG蛋白和MDM2蛋白在前列腺癌组织中的表达情况,并探讨其相关性。方法收集经病理学确诊为前列腺癌标本48例,良性前列腺增生病例10例,采用免疫组织化学二步法或SP法检测组织标本的ERG蛋白和MDM2蛋白的表达情况,分析ERG和MDM2表达与前列腺癌分级的关系。结果前列腺癌组织中ERG(E26 transformation-specific related gene,ERG)和鼠双微体-2(murine double minute-2,MDM2)蛋白阳性表达率分别为33.3%和47.9%,在良性前列腺增生组未见表达,差异有统计学意义(P0.05)。ERG和MDM2蛋白表达与前列腺癌Gleason评分分级不相关(P0.05);但ERG与MDM2蛋白在前列腺癌组织中的表达成正相关(r=0.831,P=0.001)。结论 ERG和MDM2蛋白在前列腺癌组织中的高表达,与前列腺癌的发生、发展密切相关。前列腺癌中ERG蛋白表达与MDM2蛋白的异常表达密切相关说明这两个蛋白可能存在协同作用。
[Abstract]:The first part of the Hui and Han the Ningxia Hui Autonomous Region prostate cancer risk factors Case-control study to explore the Ningxia Hui Autonomous Region Hui, prostate cancer clinical correlation between Han population factors and prevalence of prostate cancer. Methods from January 2007 to September 2013 in the General Hospital of Ningxia Medical University Hospital and diagnosed with prostate cancer in 267 patients as case group, including 214 cases of Han nationality Hui, 53 cases of patients with benign prostatic hyperplasia; 534 cases as control group, including 428 cases of Han and Hui 106 cases. By finding records collected fasting blood glucose in patients with prostate cancer (fasting blood glucose, FBG), total cholesterol (total, cholesterol, TC), triglycerides (triglyceride, TG), total prostate specific antigen (total prostate specific antigen, t-PSA), free prostate specific antigen (free prostate specific antigen, f-PSA), free And the ratio of total prostate specific antigen (f-PSA/t-PSA, f/t-PSA), the risk factors by multivariate logistic regression analysis model of prostate cancer. The risk of prostate cancer is Hui drinkers 20.48 times [35.8% non drinkers (19/53) 5.7% (6/106), OR=20.48,95%CI:4.95~84.66]; high f-PSA levels increase the risk of prostate [83.0% Hui population cancer (44/53) 55.7% (59/106), OR=4.27,95%CI:1.18~15.43]; [18.9% prostate cancer risk and high TG level reduced the Hui population (10/53) 20.8% (22/106), the risk of OR=0.24,95%CI:0.07~0.83]. in the Han population of smokers suffering from prostate cancer is 1.89 times [55.1% Han nonsmokers (118/214) 39.7% (170/428), OR=1.89,95%CI:1.28~2.78]; the high level of t-PSA [86.4% (185/214) 53.7% (230/428), OR=2.34,95%CI:1.22~ 4.52] and the high level of f-PSA [85.5% (183/214) 56.1% (240/4 28), the risk for prostate cancer OR=2.43,95%CI:1.29~4.59] will increase the Han population; and the high level of TG [15.4% (33/214) 18.7% (80/428), OR=0.59,95%CI:0.36~0.98] and high f/t-PSA ratio [53.3% (114/214) 73.4% (314/428), OR=0.53,95%CI:0.36~ 0.78] to reduce the risk for prostate cancer in Han population. Conclusion drinking and high f-PSA level is a risk the incidence of prostate cancer in Hui population factors; high TG is a protective factor for prostate cancer. The smoking, high t-PSA and high f-PSA level is a risk factor in prostate cancer; high level and high f/t-PSA ratio TG as protective factors of prostate cancer in Ningxia Han Hui, between the factors that affect the risk of prostate cancer in Han population the second part difference. ERG and MDM2 expression in prostate cancer objective study on the relationship of detection of ERG protein and MDM2 protein in prostate cancer The expression, and to explore their correlation. Methods the pathological diagnosis of prostate cancer specimens in 48 cases, 10 cases of benign prostatic hyperplasia patients, the expression of ERG protein and MDM2 protein in tissue samples was detected by immunohistochemistry in two step method or SP method, and analyze the relationship between ERG and MDM2 expression and the grade of prostate cancer results in prostate cancer ERG (E26 transformation-specific related gene, ERG) and MDM2 (murine double minute-2 -2, MDM2) protein expression rates were 33.3% and 47.9% in benign prostatic hyperplasia group no expression, the difference was statistically significant (P0.05) the expression of.ERG and MDM2 protein and Gleason score of prostate cancer no correlation (P0.05); but the expression of ERG and MDM2 protein in prostate cancer tissues was positively correlated (r=0.831, P=0.001). Conclusion the high expression of ERG and MDM2 protein in prostate cancer tissues, and The occurrence and development of prostate cancer is closely related. The expression of ERG protein in prostate cancer is closely related to the abnormal expression of MDM2 protein, indicating that these two proteins may have synergistic effects.

【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R737.25

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