FHIT、RASSF1A蛋白在食管鳞状细胞癌组织中的表达及临床意义研究

发布时间：2018-01-13 19:25

本文关键词：FHIT、RASSF1A蛋白在食管鳞状细胞癌组织中的表达及临床意义研究　出处：《兰州大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的：探讨FHIT、RASSF1A蛋白在食管鳞状细胞癌组织中的表达情况及与各临床病理指标之间的相互关系。方法：应用免疫组织化学过氧化物酶标记的链霉卵白素(Streptavidin-peroxidase, SP)的方法检测抑癌基因FHIT和RASSF1A蛋白在8例慢性食管炎、18例低级别上皮内瘤变、11例高级别上皮内瘤变及39例食管鳞状细胞癌组织中的表达情况。结果：FHIT在慢性食管炎、低级别上皮内瘤变、高级别上皮内瘤变及食管鳞状细胞癌组织中的阳性率分别为12.50%(1/8)、33.33%(6/18)、36.3696(4/11)、43.59%(17/39),各个组之间的差别没有统计学意义(P0.05)。RASSF1A在慢性食管炎、低级别上皮内瘤变、高级别上皮内瘤变及食管鳞状细胞癌组织中的阳性表达率分别是75.00%(6/8)、50.00%(9/18)、45.45%(5/11)、33.33%(13/39),只有食管鳞癌组与慢性食管炎组比较差别有统计学意义(P=0.03)。FHIT在食管鳞状细胞癌中的表达情况与TNM分期及淋巴转移显著相关,其在I+II期表达的阳性率明显的高于III期(P=0.00),在合并淋巴结转移组的阳性表达比例显著低于没有淋巴结转移组(P=0.01),而与年龄、性别、肿瘤的大小、肿瘤的生长部位、组织病理类型、浸润层次、组织分化程度等无关(P0.05)。RASSF1A在食管鳞状细胞癌中的表达水平与TNM分期及浸润层次密切相关,其在I+II期的阳性表达比例显著的高于III期(P=0.02),在没有侵犯到外膜层组的阳性率明显的高于侵犯外膜层组(P=0.02),与年龄、性别、肿瘤的大小、肿瘤的生长部位、组织病理类型、淋巴转移、组织分化程度等没有关系(P0.05)。FHIT及RASSF1A蛋白在食管鳞状细胞癌中的表达没有明显的相关性(r=-0.05,P=0.68)。结论：FHIT、RASSF1A蛋白的表达均与食管鳞癌的发生发展密切相关。FHIT蛋白可能在食管鳞癌的发生发展及侵袭转移中发挥作用,而RASSF1A蛋白的检测在食管鳞癌的临床进展及预后判断方面具有一定意义。
[Abstract]:Objective: to study FHIT. Expression of RASSF1A protein in esophageal squamous cell carcinoma and its relationship with clinicopathological parameters. Methods: streptavidin (SP) labeled by immunohistochemical peroxidase (PPO) was used to detect the expression of RASSF1A protein in esophageal squamous cell carcinoma (esophageal squamous cell carcinoma). Streptavidin-peroxidase. The tumor suppressor gene FHIT and RASSF1A protein were detected in 8 cases of chronic esophagitis and 18 cases of low grade intraepithelial neoplasia. 11 cases of high grade intraepithelial neoplasia and 39 cases of esophageal squamous cell carcinoma. Results: FHIT was detected in chronic esophagitis and low grade intraepithelial neoplasia. The positive rates of high grade intraepithelial neoplasia and esophageal squamous cell carcinoma were 12.50 / 8 and 33.336 / 18 / 36.3696 / 4 / 11, respectively. There was no statistically significant difference between the two groups in chronic esophagitis, low grade intraepithelial neoplasia. The positive expression rates in high-grade intraepithelial neoplasia and esophageal squamous cell carcinoma were 75.00 and 6- / 80.000, respectively. 33.33 / 13 / 39). Only the difference between esophageal squamous cell carcinoma group and chronic esophagitis group was statistically significant. The expression of P0.03FHIT in esophageal squamous cell carcinoma was significantly correlated with TNM stage and lymphatic metastasis. The positive rate of expression in III phase was significantly higher than that in III stage, and the positive rate in the group with lymph node metastasis was significantly lower than that in the group without lymph node metastasis (P < 0.01). But with age, sex, tumor size, tumor growth site, histopathological type, infiltration level. The expression level of P0.05U. RASSF1A in esophageal squamous cell carcinoma was closely related to TNM stage and invasive level. The percentage of positive expression in III phase was significantly higher than that in III stage, and the positive rate in the group without invasion of the outer layer was significantly higher than that in the group with invasion of the outer layer, and the positive rate was significantly higher than that in the group without invasion of the outer layer. Sex, tumor size, tumor growth site, histopathological type, lymphatic metastasis. There was no significant correlation between the expression of P0.05FHIT and RASSF1A protein in esophageal squamous cell carcinoma. Conclusion: FHIT. The expression of RASSF1A protein is closely related to the occurrence and development of esophageal squamous cell carcinoma. FHIT protein may play a role in the occurrence, development, invasion and metastasis of esophageal squamous cell carcinoma. The detection of RASSF1A protein has a certain significance in the clinical progress and prognosis of esophageal squamous cell carcinoma.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.1

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