穿膜肽修饰的纳米颗粒抗胰腺癌的初步研究

发布时间：2018-01-26 11:36

本文关键词： 穿膜肽纳米颗粒胰腺癌光动力学疗法　出处：《安徽医科大学学报》2017年10期 　论文类型：期刊论文

【摘要】：目的探究穿膜肽修饰的纳米颗粒介导的光动力学疗法(PDT)对胰腺癌SW1990细胞的杀伤作用。方法将胰腺癌SW1990细胞分成4组,不光照不含光敏剂的细胞作为空白对照组,与细胞共孵育的游离Ce6组,NP/Ce6组及TAT-NP/Ce6组。首先分别制备出纳米颗粒TAT-NP/Ce6和NP/Ce6。利用流式细胞仪(FACS)检测各组被SW1990细胞摄取的情况;荧光显微镜观察各组光照下在细胞内产生活性氧的情况;最后用MTT或活死染色法检测光照下各组对细胞的杀伤效果。结果 FACS结果显示各组分别被细胞摄取4 h后,TAT-NP/Ce6组胞内荧光显著高于NP/Ce6组(P0.001),同时NP/Ce6组明显强于free Ce6组(P0.001)。荧光显微镜显示光照下纳米颗粒TAT-NP/Ce6组在细胞内产生活性氧明显强于其他各组(P0.001)。MTT法显示在每一Ce6浓度下光照时,TAT-NP/Ce6组对细胞的杀伤效果明显强于NP/Ce6(P0.01),Ce6浓度为0.5μg/ml时,活死细胞染色法同样证明了对细胞的这种杀伤效果。结论TAT肽修饰的纳米颗粒能有效增加胰腺癌细胞对其摄取,其介导的PDT杀伤胰腺癌细胞效果明显增强。
[Abstract]:Objective to investigate the killing effect of transmembrane peptide modified nanoparticles (PDT) on pancreatic cancer SW1990 cells. Methods Pancreatic carcinoma SW1990 cells were divided into 4 groups. The cells without photoperiod and without Guang Min were used as the blank control group and the free Ce6 group co-incubated with the cells. In NP/Ce6 group and TAT-NP/Ce6 group, the nanoparticles TAT-NP/Ce6 and NPP / Ce6 were prepared, respectively. Flow cytometry (FCM) was used. The uptake of SW1990 cells in each group was detected. Fluorescence microscope was used to observe the production of reactive oxygen species (Ros) in the cells under light. Finally, MTT or live death staining was used to detect the killing effect of each group under light. Results the FACS results showed that the cells in each group were ingested for 4 hours. The intracellular fluorescence in TAT-NP/Ce6 group was significantly higher than that in NP/Ce6 group (P 0.001). At the same time, NP/Ce6 group was significantly stronger than free Ce6 group (P0.001). Fluorescence microscope showed that the reactive oxygen species (Ros) production in the TAT-NP/Ce6 group was significantly stronger than that in the other groups (P0.001). MTT method was used to show the light intensity at each Ce6 concentration. The killing effect of TAT-NP/Ce6 group was significantly better than that of NP- / Ce6P0.01Ce _ 6 concentration of 0.5 渭 g / ml. TAT peptide modified nanoparticles can effectively increase the uptake of pancreatic cancer cells. The effect of PDT mediated killing pancreatic cancer cells was significantly enhanced.
【作者单位】：安徽医科大学附属省立医院消化内科;
【基金】：安徽省自然科学基金(编号:1508085SQH224)
【分类号】：R735.9
【正文快照】： 胰腺癌被称为“癌中之王”,预后差,其5年生存率不足5%[1-2]。大多数患者被发现时已经失去了唯一可能治愈的手术机会,中晚期主要以基于吉西他滨为一线化疗药的化疗方案,效果不甚理想。光动力学疗法(photodynamic therapy,PDT)是近些年在临床上应用越来越广泛的新型疗法。目前临

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