DLL4和CD105在卵巢癌中的表达及临床意义

发布时间：2018-01-26 12:42

本文关键词： 卵巢癌 DLL4 CD105 微血管密度　出处：《青岛大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:通过检测卵巢癌及正常卵巢组织中Notch-Delta样配体(DLL4)和CD105表达,同时测定这两组患者血清中CD105的浓度,分析Notch-Delta样配体(DLL4)和CD105在卵巢癌中的意义、它们之间的联系及与卵巢癌患者的临床病理参数的关系。方法:选取来自临沂市中心医院妇科2015年11月-2017年2月期间,自术中切除的65例卵巢癌标本,同时间段手术切除的18例正常卵巢组织。所有标本需符合以下标准:1、所有患者无其它恶性肿瘤病史;2、患者病历资料完整,且附有2名病理科医师诊断的病理报告单;3、术前未进行放化疗、激素治疗等;应用免疫组化,懫用S-P法,在卵巢癌及正常卵巢组织中分别测定DLL4的表达及CD105标记的微血管密度(CD105-MVD)。同时应用酶联免疫吸附法(ELISA)检测上述所选患者的血清CD105浓度。结果:1、DLL4在卵巢癌中表达阳性,大部分定位于细胞核,呈棕黄色细颗粒,而在正常卵巢组织中基本无表达或低表达,DLL4的阳性表达率在卵巢癌患者中为78.5%,在正常卵巢组织中的阳性表达率为11.1%。2、卵巢癌中,DLL4的表达与患者的FIGO分期、卵巢癌的分化程度、淋巴结有无转移有相关(P0.01),与卵巢癌的组织学类型、患者年龄无相关(P0.05)。3、CD105在卵巢癌中的表达为阳性,主要定位于细胞浆、细胞膜,在正常卵巢组织中基本无表达或低表达。CD105-MVD在卵巢癌患者为22.58±7.34、在正常卵巢中是0.87±0.19。4、卵巢癌组织CD105与卵巢癌的分化程度、有无淋巴结转移、FIGO分期有相关性(P0.05),和卵巢癌组织学类型、患者年龄无相关性(P0.05)。5、卵巢癌血清中CD105浓度(136.8642±26.6727)明显高于正常患者血清中的浓度(26.6727±1.4111)。6、血清中CD105与卵巢癌的分化程度、有无淋巴结转移、FIGO分期有相关性(P0.05),和卵巢癌组织学类型、患者年龄无相关性(P0.05)。7、卵巢癌组织中DLL4和CD105表达的关呈正相关(r=0.818,P0.001)。8、卵巢癌组织中CD105-MVD与血清CD105呈正相关(r=0.692,P0.001)。9、卵巢癌组织中DLL4与血清CD105的浓度呈正相关(r=0.690,P0.001)。结论:DLL4、CD105在肿瘤新生血管中起重要作用。随着卵巢癌的临床进展,组织中的DLL4、CD105及血清CD105都呈现不断增高的趋势,且呈正相关,提示在卵巢癌中,DLL4可使血管密度增加,从而参与卵巢癌的发生、发展。DLL4、CD105可作为判断卵巢癌侵袭、转移的重要指标,并为抗血管治疗提供依据。
[Abstract]:Objective: to detect the expression of Notch-Delta like ligand DLL4 (DLL4) and CD105 in ovarian cancer and normal ovarian tissues, and to determine the serum CD105 concentration in these two groups at the same time. To analyze the significance of Notch-Delta like ligand DLL4) and CD105 in ovarian cancer. The relationship between them and the clinicopathological parameters of patients with ovarian cancer. Methods: from November 2015 to February 2017, the gynecology department of Linyi central hospital was selected. 65 cases of ovarian cancer were resected during operation and 18 cases of normal ovarian tissue were resected at the same time. All specimens should meet the following standard: 1. All patients had no history of malignant tumor. (2) the patient's medical records were complete, and two pathologists' pathological reports were attached. 3, no preoperative chemotherapy, chemotherapy, hormone therapy, and so on; Immunohistochemistry and S-P method were used. The expression of DLL4 and the microvessel density of CD105-MVD labeled by CD105 were measured in ovarian cancer and normal ovarian tissues, respectively. The serum CD105 concentration of the above selected patients was measured. Results: 1. The expression of DLL4 was positive in ovarian cancer, most of which were located in the nucleus and showed brown and fine granules, but there was no or low expression in normal ovarian tissues. The positive expression rate of DLL4 was 78.5 in ovarian cancer and 11.1. 2 in normal ovarian tissue. The expression of DLL4 in ovarian cancer and the FIGO stage of ovarian cancer. The degree of differentiation of ovarian cancer, lymph node metastasis has a correlation with P0.01, and the histological type of ovarian cancer, the patient age is not related to P0.05. 3. The expression of CD105 was positive in ovarian cancer, mainly located in cytoplasm and cell membrane. The expression of CD105-MVD was 22.58 卤7.34 in ovarian cancer and 0.87 卤0.19.4 in normal ovary. There was a correlation between CD105 and differentiation degree of ovarian cancer, FIGO stage with or without lymph node metastasis, and histological type of ovarian cancer. There was no correlation between age and age (P 0.05). The serum CD105 concentration of ovarian cancer was 136.8642 卤26.6727, which was significantly higher than that of normal patients (26.6727 卤1.4111). Serum CD105 was correlated with the differentiation degree of ovarian cancer, FIGO stage with or without lymph node metastasis, and histological type of ovarian cancer. The expression of DLL4 and CD105 in ovarian cancer tissues was positively correlated with the expression of P0. 818 and P0. 001. 8 in patients with ovarian cancer, there was no correlation between age and age (P0. 05. 7, P 0. 05. 7). There was a positive correlation between CD105-MVD and serum CD105 in ovarian cancer tissues. There was a positive correlation between the concentration of DLL4 and serum CD105 in ovarian cancer tissues. CD105 plays an important role in tumor neovascularization. With the clinical progress of ovarian cancer, both DLL4CD105 and serum CD105 have a positive correlation with each other. It is suggested that DLL4 can increase the density of blood vessels and participate in the development of ovarian cancer. DLL4CD105 can be used as an important index to judge the invasion and metastasis of ovarian cancer. And to provide the basis for anti-vascular therapy.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.31

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