二甲双胍和紫杉醇对卵巢癌SKOV3细胞体外增殖和凋亡的影响

发布时间：2018-01-29 20:29

本文关键词： 卵巢肿瘤二甲双胍紫杉醇细胞增殖细胞凋亡　出处：《吉林大学学报(医学版)》2017年02期 　论文类型：期刊论文

【摘要】：目的:研究二甲双胍联合紫杉醇在体外对人卵巢癌SKOV3细胞增殖和凋亡的影响,阐明二甲双胍与紫杉醇的药物协同效应。方法:将卵巢癌SKOV3细胞分为空白对照组、不同浓度(0.01、0.50、1.00、5.00和10.00mmol·L~(-1))二甲双胍组和联合用药组(不同浓度二甲双胍及紫杉醇联合用药)。MTT法检测各组SKOV3细胞增殖抑制率;流式细胞术检测各组SKOV3细胞凋亡率及细胞周期变化;MTT法检测各组SKOV3细胞增殖抑制率。结果:MTT法检测,不同浓度二甲双胍组SKOV3细胞增殖抑制率明显升高,并呈浓度和时间依赖性,与空白对照组比较差异有统计学意义(P0.05)。流式细胞术检测,不同浓度二甲双胍作用下SKOV3细胞凋亡率明显升高,与空白对照组比较,随着二甲双胍浓度的增加,药物作用48h后实验组SKOV3的细胞凋亡率明显升高(P0.05);同时细胞周期发生明显变化,随着二甲双胍浓度的增加,G0/G1期细胞百分率降低(P0.05),而G_2/M期细胞百分率升高(P0.05)。MTT法检测,1.00mmol·L~(-1)二甲双胍与紫杉醇联合用药组SKOV3细胞增殖抑制率高于0.50mmol·L~(-1)二甲双胍与紫杉醇联合用药组(P0.05);二甲双胍与紫杉醇联合用药组SKOV3细胞增殖抑制率高于单独紫杉醇组(P0.05)。结论:二甲双胍通过诱导细胞凋亡抑制卵巢癌细胞SKOV3细胞增殖,二甲双胍能够增强紫杉醇对卵巢癌SKOV3细胞增殖抑制作用,二者具有药物协同效应。
[Abstract]:Objective: to study the effect of metformin combined with paclitaxel on proliferation and apoptosis of human ovarian cancer SKOV3 cells in vitro. To elucidate the synergistic effect of metformin and paclitaxel. Methods: ovarian cancer SKOV3 cells were divided into blank control group with different concentrations of 0.01U 0.50U 1.00. 5. 00 and 10. 00 mmol 路L ~ (-1)) metformin group and combined treatment group (different concentrations of metformin and paclitaxel). The proliferation inhibition rate of SKOV3 cells in each group was determined by MTT assay. Apoptosis rate and cell cycle of SKOV3 cells in each group were detected by flow cytometry. Results the inhibition rate of SKOV3 cell proliferation in different concentrations of metformin was significantly increased in a dose-and time-dependent manner. Compared with the control group, the difference was statistically significant (P 0.05). Flow cytometry showed that the apoptotic rate of SKOV3 cells in different concentrations of metformin was significantly higher than that in the blank control group. With the increase of metformin concentration, the apoptotic rate of SKOV3 in experimental group increased significantly after 48 h of treatment. At the same time, the cell cycle changed obviously, with the increase of metformin concentration, the percentage of G _ 0 / G _ 1 phase decreased (P 0.05). However, the percentage of G _ 2 / M phase cells was increased by P0.05U. MTT assay. 1.00 mmol 路L ~ (-1)) Metformin combined with paclitaxel could inhibit the proliferation of SKOV3 cells more than 0.50 mmol 路L ~ (-1). Metformin combined with paclitaxel group (P0.05); The inhibitory rate of SKOV3 cell proliferation in metformin combined with paclitaxel group was higher than that in paclitaxel alone group (P 0.05). Conclusion: metformin inhibits the proliferation of ovarian cancer SKOV3 cells by inducing apoptosis. Metformin can enhance the inhibitory effect of paclitaxel on the proliferation of ovarian cancer SKOV3 cells.
【作者单位】：吉林大学中日联谊医院妇产科;山东省潍坊市临朐县人民医院;
【基金】：吉林省卫计委科研基金资助课题(20152034) 吉林省科技厅国际合作项目资助课题(20170414033GH)
【分类号】：R737.31
【正文快照】： 卵巢癌为妇科常见的恶性肿瘤之一,目前对于卵巢癌的治疗主要为手术联合以紫杉醇、铂类为主的化疗,但研究[1]显示其5年生存率无明显改善。很多学者致力于开发新的抗肿瘤药物,然而新型药物的研制耗时长、花费高、获得应用于临床的药物许可率低。因此,目前部分学者倾向于应用已被

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