基于iTRAQ蛋白组学技术的大肠癌和肝癌术后肝肾阴虚证血浆差异表达蛋白的研究

发布时间：2018-02-01 21:46

本文关键词： 大肠癌肝癌肝肾阴虚证蛋白质组学差异表达蛋白同位素相对标记与绝对定量技术　出处：《中华中医药杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的:筛选大肠癌和肝癌术后肝肾阴虚证的血浆差异表达蛋白,探索大肠癌和肝癌"异病同证"的物质基础。方法:纳入具有典型肝肾阴虚证的大肠癌和肝癌患者各10例,对照组为无证可辨的大肠癌和肝癌患者各10例。采集患者血浆并提取蛋白,然后进行蛋白变性、还原及酶解,最后进行同位素相对标记与绝对定量技术(i TRAQ)标记和液相色谱串联质谱联用技术鉴定,得到的峰图采用Maxquant 1.3.0.5软件进行系统的蛋白差异表达分析。鉴定蛋白组间比值1.5或0.6被认为存在表达差异。结果:大肠癌术后肝肾阴虚证组共筛选出9个典型的差异表达蛋白,肝癌术后肝肾阴虚证组也筛选出9个典型差异表达蛋白,两组共同的差异蛋白有8个:激肽原1(KNG1)、血红蛋白α2(HBA2)、血红蛋白β(HBB)、α1微球蛋白比库蛋白前体(AMBP)、性激素结合球蛋白(SHBG)、羧肽酶N催化链(CPN1)、血浆蛋白酶C1抑制剂(SERPING1)、间α胰蛋白酶抑制剂重链H1(ITHIH1)。这些蛋白主要与补体和凝血级联途径有紧密联系。结论:本研究证实i TRAQ结合液相色谱串联质谱联用技术能够快速、有效地进行差异蛋白质组学研究,发现KNG1,HBA2等蛋白可能是大肠癌和肝癌"异病同证"的物质基础之一。
[Abstract]:Objective: to screen plasma differentially expressed proteins for liver and kidney yin deficiency syndrome after operation of colorectal cancer and liver cancer. Methods: 10 cases of colorectal cancer and 10 cases of liver cancer with typical liver and kidney yin deficiency syndrome were included. The control group consisted of 10 patients with colorectal cancer and 10 patients with liver cancer. The patients' plasma was collected and protein was extracted. Then protein denaturation, reduction and enzymatic hydrolysis were performed. Isotope relative labeling, absolute quantitative technique and liquid chromatography-tandem mass spectrometry (LC-MS) were used for identification. Maxquant 1.3.0.5 software was used to analyze the differential expression of protein. The ratio of 1. 5 or 0. 6 was identified to be different. Nine typical differentially expressed proteins were screened out in patients with liver and kidney yin deficiency syndrome after colorectal cancer operation. Nine typical differentially expressed proteins were also screened out in liver and kidney yin deficiency syndrome group after liver cancer operation. There were 8 common differentially expressed proteins in the two groups: kallikinogen 1 (KNG1), hemoglobin 伪 2 (HBA2). Hemoglobin 尾 #en0#, 伪 1 microglobulin precursor AMBP, sex hormone binding globulin (SHBG), carboxypeptidase N catalytic chain (CPN 1). Plasma protease C1 inhibitor SERPING1). 伪 -trypsin inhibitor heavy chain H _ 1 _ 1 _ (ITH _ H _ 1). These proteins are closely related to complement and coagulation cascade pathway. Conclusion: I TRAQ combined with liquid chromatography-tandem mass spectrometry can be used quickly. Through effective differential proteomics study, it was found that KNG1, HBA2 and other proteins may be one of the material bases for the "syndromes of different diseases" between colorectal cancer and liver cancer.
【作者单位】：上海中医药大学复杂系统研究中心;上海中医药大学附属曙光医院;上海中医药大学附属第七人民医院肿瘤科;
【基金】：国家自然科学基金项目(No.81330084,No.81303102,No.81573749) 浦东新区卫生系统重点学科建设项目(No.PWZxq2014-12) 上海市局级课题项目(No.20134173)~~
【分类号】：R735
【正文快照】： 近些年,由于饮食方式和生活习惯的变化,大肠癌的发病率逐年上升,升幅越居榜首[1]。ⅡB期以上或具有高危因素的Ⅱ期大肠癌术后患者化疗和(或)放疗后仍有约50%患者于根治术后2年内出现复发或转移。中医认为,大肠癌患者临床常见证型有湿热蕴结型、瘀毒内阻型、脾虚气滞型、肝肾阴

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