GPR124在胃癌组织中的表达及意义

发布时间：2018-02-02 20:55

本文关键词： 胃癌 GPR124 临床意义　出处：《南华大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的:检测GPR124在胃癌组织中的表达情况,分析其临床病理意义,为胃癌的早期诊断和分子靶向药物治疗提供有价值的参考。方法:收集20例胃癌、癌旁组织和正常组织的新鲜标本,提取组织蛋白和总RNA,分别运用Western blot和RT-PCR检测GPR124蛋白和RNA在上述不同组织中的表达情况。同时收集不同分化腺癌、非典型增生、正常胃组织石蜡标本共99例,运用免疫组织化学染色检测不同胃组织中GPR124蛋白的阳性率。分析GPR124蛋白在不同胃组织中表达的差别及意义。结果:1、Western blot检测结果:GPR124蛋白在胃癌组织中的表达明显高于癌旁组织和正常组织(P0.05),癌旁组织也高于正常组织(P0.05)。2、RT-PCR检测结果:GPR124在胃癌组织中的表达明显高于癌旁组织和正常组织(P0.05),癌旁组织也高于正常组织(P0.05)。3、免疫组化染色检测结果:GPR124蛋白主要表达于胞膜,在胃癌组织、非典型增生组织、正常组织的阳性表达率分别为77.78%(49/63)、30.77%(4/13)、4.35%(1/23)。GPR124蛋白在癌组织中的阳性表达率显著高于非典型增生组织和正常组织(P0.05),非典型增生组织的阳性表达率表达高于正常组织(P0.05);另外,GPR124蛋白在高分化、中分化、低分化胃癌组织中的阳性表达率分别为33.33%(4/12)、75.00%(15/20)、96.77%(30/31),三组比较均有统计学意义(P0.05);III~IV期胃癌的阳性表达率明显高于I~II期,伴淋巴结转移胃癌的阳性表达率也明显高于无转移者。结论:1、GPR124蛋白在胃癌组织中表达上调。2、GPR124蛋白的表达水平与胃癌的分化程度、临床病理分期及是否伴淋巴结转移有关。
[Abstract]:Objective: to detect the expression of GPR124 in gastric cancer and analyze its clinicopathological significance, and to provide valuable reference for early diagnosis and molecular targeted drug therapy of gastric cancer. Methods: 20 cases of gastric cancer were collected. Tissue proteins and total RNA were extracted from fresh specimens of paracancerous tissues and normal tissues. Western blot and RT-PCR were used to detect the expression of GPR124 protein and RNA in different tissues, and different differentiated adenocarcinoma and atypical hyperplasia were collected. There were 99 cases of paraffin wax in normal gastric tissue. Immunohistochemical staining was used to detect the positive rate of GPR124 protein in different gastric tissues. The difference and significance of the expression of GPR124 protein in different gastric tissues were analyzed. Results: 1. The results of Western blot analysis showed that the expression of GPR124 protein in gastric cancer was significantly higher than that in paracancerous tissues and normal tissues (P0.05). The expression of TGPR124 in gastric cancer tissues was significantly higher than that in adjacent tissues and normal tissues (P0.05). The results of immunohistochemical staining showed that the protein of 1% GPR124 was mainly expressed in the cell membrane, and in gastric cancer tissue and atypical hyperplasia tissue. The positive expression rates in normal tissues were 77.78% and 30.77%, respectively. The positive expression rate of GPR124 protein in cancer tissues was significantly higher than that in atypical hyperplasia tissues and normal tissues (P0.05). The positive expression rate in atypical hyperplasia tissues was higher than that in normal tissues. In addition, the positive expression rates of GPR124 protein in well-differentiated, moderately differentiated and poorly differentiated gastric cancer tissues were 33.33 / 12 / 75.00 / 20 respectively. 96.7777% 30% 31%, all the three groups had statistical significance (P 0.05). The positive expression rate of gastric cancer in III~IV stage was significantly higher than that in stage II, and the positive expression rate of gastric carcinoma with lymph node metastasis was significantly higher than that in patients without metastasis. Conclusion: 1. The expression of GPR124 protein in gastric carcinoma was related to the degree of differentiation, clinicopathologic stage and lymph node metastasis.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.2

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