VEGF、CD31、CD105在子宫内膜癌中的表达及其临床意义

发布时间：2018-02-04 03:57

本文关键词： 子宫内膜癌血管生成微血管密度血管内皮生长因子　出处：《广西医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:检测血管内皮生长因子(vascular endothelial growth factor,VEGF)、血小板内皮细胞粘附分子(Platelet endothelial cell adhesion molecule-1,PECAM-1/CD31)、CD105在子宫内膜癌中的表达及与血管生成的关系,分析其与子宫内膜癌的临床病理关系。方法:收集广西医科大学第一附属医院2012年至2015年子宫内膜癌组织标本52例、子宫内膜不典型增生组织标本22例及正常子宫内膜标本21例,标本均采用免疫组化方法检测VEGF、CD31、CD105在组织中的表达及计数CD31、CD105在组织表达中的微血管密度(microvessel density,MVD),对结果进行量化分析,并且分析VEGF、CD31、CD105的表达与子宫内膜癌的临床病理关系。结果:1.VEGF在正常子宫内膜组织、不典型增生及子宫内膜癌中表达的阳性率分别为23.8%、31.8%、65.4%。三者之间差异有统计学意义(P0.05)。VEGF在子宫内膜癌中的表达,与淋巴结转移相关(P0.05)。2.CD31在正常子宫内膜组织、不典型增生及子宫内膜癌组织中表达的MVD分别为23.6±11.7、31.5±17.7、51.9±21.1,三者之间有统计学差异(P0.05)。CD31在子宫内膜癌中标记的MVD与肿瘤分期、浸润肌层深度、组织学类型相关(P0.05),与患者年龄、是否绝经、肿瘤大小、有无淋巴结转移及CA125血清水平无关(P0.05)。3.CD105在正常子宫内膜组织、不典型增生及子宫内膜癌组织中表达的MVD分别为3.6±3.3、4.1±3.0、6.2±2.9,三者之间有统计学差异(P0.05)。CD105在子宫内膜癌中标记的MVD,与有无淋巴结转移及肿瘤大小相关(P0.05),与其他临床病理特征无关(P0.05)。4.在子宫内膜癌组,VEGF表达与CD31、CD105标记的MVD均无明显相关性(r=0.095,0.031;P=0.504,0.826)。CD31与CD105标记的MVD无明显相关性(r=0.046;P=0.749)。CD31标记的MVD明显高于CD105标记的MVD(P0.05)。结论:VEGF、CD31、CD105在子宫内膜癌组织中表达升高,可能促进子宫内膜癌的血管生成,它们作为子宫内膜癌的肿瘤标志物有一定的临床价值。
[Abstract]:Objective: to detect vascular endothelial growth factor (VEGF). Platelet endothelial cell adhesion molecule-1. Expression of PECAM-1 / CD31 / CD105 in endometrial carcinoma and its relationship with angiogenesis. Methods: 52 specimens of endometrial carcinoma were collected from 2012 to 2015 in the first affiliated Hospital of Guangxi Medical University. 22 cases of atypical hyperplasia of endometrium and 21 cases of normal endometrium were detected by immunohistochemical method. Expression of CD105 in tissues and count of microvessel density (MVD) of CD31 and CD105 in tissue expression. Quantitative analysis of the results and analysis of VEGF CD31 CD105 expression and endometrial carcinoma clinicopathological relationship. Results 1. VEGF in normal endometrial tissue. The positive rates of expression in atypical hyperplasia and endometrial carcinoma were 23.810% and 31.8%, respectively. There was significant difference between the three groups in the expression of P0.05. VEGF in endometrial carcinoma. The expression of MVD in normal endometrium, atypical hyperplasia and endometrial carcinoma was 23.6 卤11.7. 31.5 卤17.7 卤51.9 卤21.1, there was statistical difference between the three groups. P0.05U. CD31 was marked in endometrial carcinoma with MVD, tumor stage, depth of infiltrating myometrium. Histologic types were correlated with P0.05, with age, menopause, and tumor size. There was no correlation between lymph node metastasis and serum level of CA125 in normal endometrium. The expression of MVD in atypical hyperplasia and endometrial carcinoma was 3.6 卤3.30.4.1 卤3.0 卤6.2 卤2.9, respectively. There was a statistical difference among the three groups (P 0.05. CD105 was associated with lymph node metastasis and tumor size (P 0. 05)) in endometrial carcinoma. There was no significant correlation between the expression of MVD in endometrial carcinoma group and the MVD labeled by CD31or CD105.There was no significant correlation between VEGF expression and CD31or CD105labeled MVD in endometrial carcinoma group. 0.031; There was no significant correlation between MVD labeled with CD105 and 0.504T 0.826n.CD31. The MVD labeled by CD105 was significantly higher than that by P0. 749. CD31. Conclusion\\\% VEGF\% CD31\%\% VEGF\ +\%\%\%\%\%\%\% VEGF\%\%\%? The increased expression of CD105 in endometrial carcinoma may promote the angiogenesis of endometrial carcinoma, and they may be used as tumor markers of endometrial carcinoma.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33

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