棕榈酸刺激的巨噬细胞对HepG2细胞侵袭和迁移的影响

发布时间：2018-02-04 16:03

本文关键词： 棕榈酸 HepG细胞巨噬细胞炎症因子细胞侵袭细胞迁移　出处：《中国病理生理杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:研究棕榈酸刺激的巨噬细胞对肝癌细胞侵袭和迁移能力的影响,并进一步探讨其作用机制。方法:应用棕榈酸(0.16 mmol/L)刺激人急性单核细胞白血病细胞系THP-1来源的巨噬细胞,并取其上清液处理HepG2细胞。细胞迁移实验检测巨噬细胞的迁移能力,侵袭实验和划痕实验分别观察HepG2细胞的纵向迁移能力和横向迁移能力;RT-qPCR检测巨噬细胞和HepG2细胞的炎症/趋化因子及HepG2细胞上皮-间充质转化标志蛋白(E-cadherin和N-cadherin)的mRNA表达水平。结果:棕榈酸促进了巨噬细胞迁移,显著上调巨噬细胞白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)和单核细胞趋化蛋白1(MCP-1)的mRNA表达;用棕榈酸刺激巨噬细胞的上清液处理HepG2细胞,其纵向迁移能力和横向迁移力明显强于未经棕榈酸处理组,且HepG2细胞的多种炎症因子和N-cadherin表达上调,E-cadherin表达下调。结论:棕榈酸可以增强巨噬细胞的迁移能力,刺激巨噬细胞产生大量炎症因子/趋化因子,进一步通过旁分泌/内分泌作用于HepG2细胞,促进HepG2细胞的上皮-间充质转化,增强HepG2细胞的侵袭与迁移能力。
[Abstract]:Objective: to study the effect of palmitic acid-stimulated macrophages on the invasion and migration of hepatoma cells. Methods: the macrophages derived from human acute monocytic leukemia cell line THP-1 were stimulated with palmitic acid 0.16 mmol / L. The migration ability of macrophages was detected by cell migration assay, and the longitudinal migration ability and lateral migration ability of HepG2 cells were observed by invasion assay and scratch test, respectively. RT-qPCR was used to detect inflammatory / chemokine and HepG2 epithelial-mesenchymal transformation markers in macrophages and HepG2 cells. MRNA expression levels of E-cadherin and N-cadherin. Results: palmitic acid promoted the migration of macrophages. The levels of IL-1 尾 -IL-1 尾 and IL-6 in macrophages were significantly up-regulated. MRNA expression of TNF- 伪 and MCP-1; When HepG2 cells were treated with palmitic acid-stimulated supernatant, the ability of longitudinal migration and lateral migration was significantly stronger than that of non-palmitic acid treated group. The expression of N-cadherin and inflammatory factors in HepG2 cells were up-regulated and down-regulated. Conclusion: palmitic acid can enhance the migration ability of macrophages. Stimulating macrophages to produce a large number of inflammatory factors / chemokines, further through paracrine / endocrine action on HepG2 cells, promote the HepG2 cells epithelial-mesenchymal transformation. Enhance the ability of invasion and migration of HepG2 cells.
【作者单位】：重庆医科大学脂糖代谢性疾病重庆市重点实验室脂质研究中心;
【基金】：国家自然科学基金资助项目(No.31540029) 重庆市科委科技计划(No.cstc2016jcyj A0545)
【分类号】：R735.7
【正文快照】： 肝细胞癌(hepatocellular carcinoma,HCC)是一种常见的高致死率恶性肿瘤之一,是我国癌症死亡的第二大原因。影响肝癌患者预后的因素众多,但肿瘤的侵袭和转移是影响肝癌患者预后的最关键因素之一。近年研究提示肥胖及糖尿病患者的乳腺癌、前列腺癌、肝癌等发病率高于正常对照组

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