c-Jun基因启动子区多态性位点rs4646999与肠癌预后的相关性

发布时间：2018-02-04 19:03

本文关键词： 结直肠癌单核苷酸多态性生存预后 c-Jun基因　出处：《实用医学杂志》2017年21期 　论文类型：期刊论文

【摘要】：目的探讨c-Jun基因启动子多态性位点rs4646999与肠癌预后的相关性。方法采用Taq-Man探针法检测436例肠癌患者rs4646999-673CT的基因型,通过Kaplan-Meier法和Cox回归分析其生存曲线;采用免疫印迹分析多态性位点不同基因型的c-Jun蛋白表达水平。结果单因素分析表明携带TT基因型患者累积生存率显著高于CT和CC基因型携带者,差异有统计学意义。多因素Cox回归分析显示分化程度、淋巴结转移、远处转移、TNM分期和rs4646999位点基因型为预后因素,CT/CC基因型与肠癌较差的生存预后相关(P0.05)。蛋白表达分析发现CC基因型癌组织c-Jun蛋白表达升高,而TT基因型则相反。结论 c-Jun基因启动子区域多态性位点rs4646999与肠癌生存预后相关,其作用机制可能是增强c-Jun蛋白表达导致肠癌发生发展。
[Abstract]:Objective to investigate the correlation between c-Jun gene promoter polymorphism rs4646999 and prognosis of colorectal cancer. Methods rs464699 was detected by Taq-Man probe method in 436 patients with colorectal cancer. The genotype of 9-673CT. The survival curve was analyzed by Kaplan-Meier method and Cox regression analysis. Results univariate analysis showed that the cumulative survival rate of patients with TT genotype was significantly higher than that of patients with CT and CC genotypes. Multivariate Cox regression analysis showed that the degree of differentiation, lymph node metastasis, distant metastasis, TNM staging and rs4646999 locus genotype were prognostic factors. CT/CC genotypes were associated with poor survival and prognosis of colorectal cancer. Protein expression analysis showed that c-Jun protein expression was increased in CC genotype carcinoma. Conclusion the polymorphism of c-Jun gene promoter region rs4646999 is associated with the survival and prognosis of colorectal cancer. Its mechanism may be to enhance the expression of c-Jun protein, leading to the carcinogenesis and development of colorectal cancer.
【作者单位】：中山大学附属第六医院肿瘤内科;中山大学附属第六医院结直肠外科;广东省结直肠盆底疾病研究重点实验室;
【基金】：国家自然科学基金青年项目(编号:81201581) 广东省自然科学基金面上项目(编号:S2013010013478) 广州市科技计划项目(编号:201707010430)
【分类号】：R735.3
【正文快照】： 结直肠癌是最常见的恶性肿瘤之一,目前已跃居常见肿瘤第三位,全球每年新发病例近120万、死亡人数达60万[1]。在中国结直肠癌的发病率呈逐渐上升的趋势,尤其近年更以4%速度迅猛增长,严重威胁人们的健康和生命[2]。由于结直肠癌早期症状不明显,无特异性,多数患者在初诊时已处

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