斑马鱼异种异体急性髓系白血病模型的建立

发布时间：2018-02-05 00:22

本文关键词： 斑马鱼急性髓系白血病白血病干细胞动物模型 KG-a细胞　出处：《中国实验血液学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨利用人急性髓系白血病干细胞和斑马鱼建立斑马鱼白血病模型,为白血病的研究及治疗药物的筛选提供更为直接的体外研究模型和体内实验依据。方法:利用MitoRed染料对白血病干样细胞KG-1a细胞进行标记,然后通过显微注射法把KG-1a细胞植入斑马鱼胚胎的卵黄囊中,观察移植后斑马鱼胚胎的大体存活状态、细胞在斑马鱼体内的增殖和扩散转移;应用组织学切片观察移植后细胞对斑马鱼组织器官的侵袭作用。结果:KG-1a细胞植入斑马鱼胚胎的卵黄囊后,MitoRed标记的KG-1a细胞数目随时间延长而增多并逐渐扩散至整个腹腔,体外计数结果也显示植入细胞明显增殖,表明植入的白血病干细胞能够在斑马鱼体内存活,增殖并扩散。进一步的研究显示,在斑马鱼肝脏组织发现有白血病细胞的浸润,HE染色提示浸润细胞具有KG-1a细胞核型特征。结论:异种异体的人急性髓系白血病干细胞KG-1a可以植入斑马鱼体内并成功建立斑马鱼的白血病模型。该模型的建立可为白血病的研究及治疗药物的筛选提供一种更为直接高效的体外研究平台。
[Abstract]:Objective: to establish a model of zebrafish leukemia using human acute myeloid leukemia stem cells and zebrafish. To provide a more direct in vitro research model and in vivo experimental basis for the study of leukemia and the screening of therapeutic drugs. Methods: KG-1a cells were labeled with MitoRed dye. Then the KG-1a cells were implanted into the yolk sac of zebrafish embryos by microinjection to observe the survival state of zebrafish embryos and the proliferation and diffusion of the cells in zebrafish. Histological sections were used to observe the invasion of zebrafish tissues and organs after transplantation. Results\\\% KG-1a cells were implanted into the yolk sac of zebrafish embryos. The number of KG-1a cells labeled with MitoRed increased with time and gradually spread to the whole abdominal cavity. The results of in vitro counting also showed that the implanted cells proliferated obviously. Further studies have shown that leukemic stem cells implanted in zebrafish can survive, proliferate and spread in zebrafish. Further studies have shown that leukemic cells infiltrate in the liver of zebrafish. He staining showed that the infiltrating cells had the nuclear type of KG-1a. Xenogeneic human acute myeloid leukemia stem cells (KG-1a) can be implanted into zebrafish and successfully establish the leukemia model of zebrafish. A more direct and efficient in vitro research platform.
【作者单位】：中山大学附属中山医院中山市人民医院血液内科;
【分类号】：R733.71;R-332
【正文快照】： 白血病的体外研究和临床药物的筛选离不开动物模型实验,所有的脊椎生物都有发生白血病的可能,利用脊椎动物作为研究模型,对于了解人类白血病的发生和进展以及药物的研发都具有非常重要的意义。斑马鱼(Zebrafish,Danio rerio)作为一种新型模式生物系统,具有脊椎动物模型和哺乳

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