DNA甲基转移酶在宫颈癌组织中的表达及其与HPV的相关性分析

发布时间：2018-02-07 12:07

本文关键词： 宫颈肿瘤甲基转移酶类人乳头瘤病毒　出处：《重庆医学》2015年13期 　论文类型：期刊论文

【摘要】：目的探讨5种DNA甲基转移酶(DNMTs)在宫颈癌组织中的表达情况及其与高危型人乳头瘤病毒(HPV)感染的关系。方法应用荧光定量PCR、Western blotting、免疫组化检测40例宫颈癌组织、对应癌旁组织及20例正常宫颈组织中DNMT1、DNMT2、DNMT3a、DNMT3b和DNMT3l的表达情况,应用质谱法检测HPV感染情况。结果宫颈癌组织中DNMT1、DNMT3a、DNMT3b、DNMT3l的阳性表达率分别为90.0%、85.0%、70.0%、80.0%,在癌旁组织内的阳性表达率分别为87.5%、85.0%、72.5%、77.5%,均明显高于正常宫颈组织内的阳性表达率(分别为25.0%、15.0%、20.0%和30.0%),而DNMT2在宫颈癌和癌旁组织中阳性表达率仅为12.5%和17.5%,明显低于正常宫颈组织(约85.0%),且DNMT1、DNMT3a、DNMT3b与HPV阳性呈显著正相关(r值分别为0.709、0.538和0.536,P0.05)。结论宫颈癌组织中DNMT的表达谱发生明显改变,且HPV高危亚型感染与DNMT1、DNMT3a、DNMT3b的表达升高密切相关。
[Abstract]:Objective to investigate the expression of five DNA methyltransferases (DNA) in cervical carcinoma and its relationship with high risk human papillomavirus (HPV) infection. Corresponding to the expression of DNMT1, DNMT2, DNMT3b and DNMT3l in adjacent tissues and 20 normal cervical tissues, DNMT3b, DNMT3b and DNMT3b were detected in 20 normal cervical tissues. Results the positive expression rates of DNMT1DNMT3a+ DNMT3bnm3l in cervical carcinoma tissues were 90.0 and 85.0%, respectively. The positive expression rates in adjacent tissues were 87.5% and 87.5%, respectively, which were significantly higher than those in normal cervix tissues (77.5%, respectively). (2) the positive expression rates of DNMT1 and DNMT3b in cervical cancer tissues were significantly higher than those in normal cervix tissues (77.5%, 87.5% and 87.5%, respectively). The positive expression rates of DNMT2 in cervical cancer and adjacent tissues were 12.5% and 17.5respectively, which were significantly lower than those in normal cervix tissues (about 85.0%), and the positive correlation r values between DNMT1 and DNMT3b and HPV were 0.709, 0.538 and 0.536, respectively. Conclusion\\\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\% DNMT2\%\%\%? The spectrum has changed significantly. The high risk subtype infection of HPV was closely related to the increased expression of DNMT3b in DNMT1, DNMT3a- and DNMT3b.
【作者单位】：第三军医大学大坪医院野战外科研究所妇产科;
【基金】：国家自然科学基金资助项目(81272865) 重庆市自然科学基金项目(cstc2012jjB10025)
【分类号】：R737.33

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