长链非编码RNA LINC00261在Barrett食管、食管腺癌组织中的表达及其作用

发布时间：2018-02-09 08:18

本文关键词： 长链非编码RNA LINC Barrett食管 FOXA 干细胞　出处：《第三军医大学学报》2017年01期 　论文类型：期刊论文

【摘要】：目的探讨长链非编码RNA LINC00261在Barrett食管、食管腺癌组织中的表达及其可能作用机制。方法利用qRT-PCR检测LINC00261在中国人群正常食管鳞状上皮、Barrett食管及食管腺癌组织标本中的表达水平变化。并建立了以人胚胎干细胞为细胞模型。结果在Barrett食管及食管腺癌组织标本中,LINC00261表达较正常食管鳞状上皮组织均显著上调(P0.01);酸暴露及胆酸可诱导人胚胎干细胞LINC00261表达显著上调并抑制FOXA2蛋白表达水平(P0.05),而LINC00261慢病毒载体转染亦可诱导FOXA2蛋白表达水平下调;上调FOXA2表达可阻断人胚胎干细胞在酸暴露及胆酸诱导下CDX2表达及细胞分化表型(柱状上皮标志物MUC2表达)的变化。结论 LINC00261在Barrett食管的发生过程中发挥关键作用,其可能通过抑制FOXA2表达参与相关调控。
[Abstract]:Objective to investigate the long chain noncoding RNA LINC00261 in Barrett esophagus. Methods qRT-PCR was used to detect the expression of LINC00261 in normal esophageal squamous epithelium Barrett's esophagus and esophageal adenocarcinoma. Results in Barrett esophagus and esophageal adenocarcinoma tissues, the expression of linC00261 was significantly up-regulated than that of normal esophageal squamous epithelium, and acid exposure and cholic acid could induce LINC00261 expression in human embryonic stem cells and inhibit FOXA2 significantly. LINC00261 lentivirus vector transfection could induce down-regulation of FOXA2 protein expression. Upregulation of FOXA2 expression can block the changes of CDX2 expression and differentiation phenotype (MUC2 expression of columnar epithelial marker) in human embryonic stem cells after acid exposure and cholic acid-induced. Conclusion LINC00261 plays a key role in the pathogenesis of Barrett esophagus. It may be involved in the regulation by inhibiting the expression of FOXA2.
【作者单位】：河北北方学院;解放军空军总医院消化内科/全军小肠疾病内镜诊疗中心;武汉大学中南医院消化内科;第三军医大学西南医院全军消化病研究所;
【基金】：国家自然科学基金面上项目(81470818,81170356,81270450) 解放军空军总医院课题(2014020) 河北省研究生创新项目(207)~~
【分类号】：R735.1;R571

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