广西贵港地区肝癌筛查及乙型肝炎病毒基因型分布研究

发布时间：2018-02-10 21:05

  本文关键词： 原发性肝癌 乙型肝炎病毒 基因型 筛查　出处：《广西医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨广西贵港地区防癌筛查对象的乙型肝炎病毒(HBV)感染情况和HBV基因型的分布特征,为开展肝癌早诊早治项目工作提供依据。方法:整理并分析贵港三个区(港南区、港北区、覃塘区)30~59岁居民接受肝癌筛查的数据,本次共有150124人接受筛查,其中HBsAg阳性者有18005人,从中对1989例HBsAg阳性者的血清进行HBV DNA定量检测,以选取HBV DNA病毒拷贝数大于检出限(1×103)的HBs Ag携带者,进一步运用聚合酶链反应-限制性片段长度多态性法(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)进行HBV基因分型。结果:筛查人群中HBsAg阳性率为11.99%,男性HBsAg阳性率高于女性(P0.01),不同年龄组HBsAg阳性率差异有统计学意义(P0.01),HBs Ag阳性率随着年龄的增长而呈下降趋势。在18005例肝癌高危人群中,AFP阳性414例,阳性率为2.30%;共发现58例肝癌新病例,其中早期肝癌为13例,早期诊断率为22.41%,中晚期肝癌为45例。在肝癌高危人群中,HBV DNA病毒拷贝数≥1×103为755例,其中HBV A基因型6例(0.79%),B基因型337例(44.64%),C基因型293例(38.81%),D基因型18例(2.39%),UT(Untypable)基因型101例(13.37%),B和C基因型在男女性中的分布无统计学意义(P均0.05);各年龄组间HBV基因型的分布差异无统计学意义(P均0.05)。结论:广西贵港三个区肝癌筛查人群的HBV感染率为11.99%,HBV基因型以B和C型为主。
[Abstract]:Objective: to investigate the hepatitis B virus hepatitis B virus (HBV) infection and the distribution characteristics of HBV genotypes among the cancer prevention screening subjects in Guigang, Guangxi, so as to provide the basis for the project of early diagnosis and early treatment of liver cancer. A total of 150124 people were screened for liver cancer at the age of 59 years in Qantang District, Qantang District, and 18005 of them were HBsAg positive. HBV DNA was quantitatively detected in the sera of 1989 HBsAg positive patients. In order to select HBs Ag carriers with HBV DNA virus copy number greater than the detection limit of 1 脳 103. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLPs) was further used to type HBV gene. Results: the positive rate of HBsAg was 11.99 in the screening population. The positive rate of HBsAg in males was higher than that in females, and HBsAg was positive in different age groups. The positive rate of HBs Ag decreased with the increase of age. In 18005 cases of high risk population for liver cancer, 414 cases were positive for AFP. The positive rate was 2.30%, 58 new cases of HCC were found, including 13 cases of early HCC, 22.41 cases of early diagnosis and 45 cases of advanced HCC. In the high risk population of HCC, the copy number of HBV DNA virus 鈮，

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