和厚朴酚对人肺癌A2细胞增殖和凋亡的影响

发布时间：2018-02-11 19:20

本文关键词： 和厚朴酚人肺癌A细胞细胞凋亡 Bax Bcl-　出处：《基因组学与应用生物学》2017年05期 　论文类型：期刊论文

【摘要】：本研究的目的在于研究不同浓度和厚朴酚对人肺癌A2细胞增殖和凋亡的影响,分析凋亡的可能机制。通过台盼蓝拒染法检测和厚朴酚对细胞增殖的影响,荧光显微镜下观察细胞形态的变化,流式细胞术检测细胞凋亡率和细胞周期的变化,qRT-PCR和Western blotting分别检测Bax、Bcl-2基因mRNA和蛋白表达情况。结果表明,在一定范围内,和厚朴酚对人肺癌A2细胞增殖有抑制作用且呈时间和剂量依赖性,其作用人肺癌A2细胞24 h、48 h和72 h的IC50值分别为44.03 nmol/L、26.51 nmol/L和19.54 nmol/L。不同浓度的和厚朴酚作用人肺癌A2细胞48 h后,细胞出现明显的凋亡特征,早期凋亡细胞增多,且G1期和G2期细胞减少,S期细胞增多。与对照组相比,Bax mRNA和蛋白表达均显著升高(p0.05),Bcl-2mRNA和蛋白表达均显著降低(p0.05)。本研究表明一定浓度范围的和厚朴酚能抑制人肺癌A2细胞增殖,诱导细胞凋亡且呈时间和剂量依赖性,并可上调Bax基因表达,下调Bc L-2基因表达。
[Abstract]:The purpose of this research is to study the effects of different concentrations of honokiol on proliferation and apoptosis of human lung cancer A2 cells, the mechanism of apoptosis analysis. By trypan blue staining was used to detect the effect of honokiol on cell proliferation, morphological changes were observed under the fluorescence microscope, changes of flow cytometry to detect the apoptosis rate and the cell cycle, qRT-PCR and Western blotting respectively to detect Bax expression of Bcl-2 gene mRNA and protein. The results showed that in a certain range, inhibited in a time and dose-dependent manner of honokiol on human lung cancer A2 cell proliferation and its role in human lung cancer cell line A2 24 h, 48 h and 72 h the IC50 values were 44.03 nmol/L, 26.51 nmol/L and 19.54 nmol/L. of different concentration and effect of magnolol on human lung cancer A2 cells after 48 h, apoptosis of cells, early apoptotic cells increased, and the cells in G1 phase and G2 phase decrease, S phase Cells were increased. Compared with the control group, the expression of Bax mRNA and protein were significantly increased (P0.05), Bcl-2mRNA and protein expression were significantly decreased (P0.05). This study shows that a certain concentration of honokiol can inhibit the proliferation of human lung cancer A2 cells, induced apoptosis in a dose and time dependent manner, and can be up-regulated Bax gene, expression of Bc L-2 gene.

【作者单位】：齐齐哈尔大学生命科学与农林学院;
【基金】：黑龙江省自然科学基金(批准号:C201241,C200624) 黑龙江省教育厅科学基础业务专项(批准号:135109104);黑龙江省教育厅科学技术项目(批准号:12511611/11511447)共同资助
【分类号】：R734.2

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