维生素C联合三氧化二砷对膀胱癌T-24细胞株的作用

发布时间：2018-02-12 01:24

本文关键词： T-细胞维生素C 三氧化二砷 caspase- survivin　出处：《肿瘤防治研究》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨维生素C联合三氧化二砷(As_2O_3)对膀胱癌T-24细胞的影响及其机制。方法体外培养T-24细胞,分为6组,对照组不做处理,实验组分别加入不同浓度的As_2O_3(0.4、4、40μg/ml)组,维生素C(400μg/ml)组、维生素C(400μg/ml)+As_2O_3(0.4μg/ml)组(联合组),采用细胞增殖曲线检测T-24细胞生长差异情况,用流式细胞术检测细胞周期及凋亡率变化,RT-PCR、Western blot技术检测分析caspase-3、survivin mRNA及蛋白的表达情况。结果联合组、维生素C(400μg/ml)组、As_2O_3(4μg/ml)组及As_2O_3(40μg/ml)组均对膀胱癌T-24细胞增殖有显著抑制作用;联合组将细胞阻止在G_0/G_1期,且凋亡率显著高于其他五组(P0.05);RT-PCR及Western blot结果显示,维生素C(400μg/ml)组、联合组的caspase-3 mRNA和蛋白表达升高,survivin mRNA和蛋白表达降低。结论维生素C联合As_2O_3可抑制膀胱癌T-24细胞增殖,促进细胞凋亡,其机制可能与上调caspase-3 mRNA、蛋白,下调survivin mRNA、蛋白表达有关。
[Abstract]:Objective to investigate the effect of vitamin C combined with arsenic trioxide (as _ 2O _ 3) on bladder cancer T-24 cells and its mechanism. Methods T-24 cells were cultured in vitro and divided into 6 groups without treatment in the control group. The experimental group was treated with 40 渭 g / ml of as _ 2O _ 3C _ 2O _ 3 0.40 渭 g / ml and vitamin C was 400 渭 g / ml, respectively. The cell proliferation curve was used to detect the growth difference of T-24 cells, and the changes of cell cycle and apoptosis rate were detected by flow cytometry. The expression of caspase-3 survivin mRNA and protein in the combined group was detected by RT-PCRG-Western blot. Both the As2O4 渭 g / ml group and the As_2O_3(40 渭 g / ml group significantly inhibited the proliferation of T-24 cells in the vitamin C 400 渭 g / ml group, and the apoptosis rate in the combined group was significantly higher than that in the other five groups (P 0.05 RT-PCR and Western blot). Conclusion Vitamin C combined with As_2O_3 can inhibit the proliferation of bladder cancer T-24 cells and promote cell apoptosis. The mechanism may be related to up-regulation of caspase-3 mRNAs, proteins, down-regulation of survivin mRNAs, protein expression.
【作者单位】：运城市中心医院急诊科;桂林医学院附属医院泌尿外科;桂林医学院生物技术学院;运城市中心医院泌尿外科;
【基金】：山西省青年科技研究基金(2014021037-8)
【分类号】：R737.14

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