肺腺癌中缺氧诱导因子-1α调控程序性死亡因子配体1的表达

发布时间：2018-02-22 06:06

本文关键词： 缺氧诱导因子-α 程序性死亡因子配体肺腺癌　出处：《山东大学学报(医学版)》2017年04期 　论文类型：期刊论文

【摘要】：目的研究缺氧诱导因子-1α(HIF-1α)和程序性死亡因子配体1(PD-L1)在肺腺癌中的表达与临床病理特征的关系,探讨肺腺癌中HIF-1α对PD-L1的调控作用。方法应用免疫组织化学方法检测99例肺腺癌组织中HIF-1α、PD-L1的表达。Co Cl2模拟化学性缺氧处理肺腺癌细胞,并将其分为正常组及CoCl_2组,qRT-PCR检测HIF-1α、PD-L1 mRNA表达,Western blotting检测HIF-1α、PD-L1蛋白表达。siRNA-HIF-1α瞬时转染肺腺癌细胞,并将其分为阴性对照组及siR-HIF-1α组,qRT-PCR、Western blotting检测转染效率及PD-L1 mRNA和蛋白的表达。结果 99例肺腺癌组织中,HIF-1α和PD-L1的阳性率分别为47.48%和34.34%,HIF-1α表达水平与患者年龄和临床分期有关(P0.05),PD-L1表达水平与患者临床分期有关(P0.05),HIF-1α蛋白表达与PD-L1蛋白表达呈正相关(P0.01)。与正常组相比,CoCl_2组细胞中HIF-1α蛋白表达水平明显升高(t=-2.394,P=0.029),PD-L1 mRNA及蛋白表达水平随之明显升高(t=-3.146,P=0.006;t=-3.308,P=0.004)。与阴性对照组相比,siR-HIF-1α组沉默处理细胞后,PD-L1 mRNA及蛋白表达水平显著降低(t=2.746,P=0.014;t=2.339,P=0.033)。结论肺腺癌组织中HIF-1α和PD-L1明显共表达;PD-L1表达受HIF-1α调控。
[Abstract]:Objective to study the relationship between the expression of hypoxia inducible factor-1 伪 (HIF-1 伪) and programmed death factor ligands 1 (PD-L1) in lung adenocarcinoma and the clinicopathological features. To investigate the regulatory effect of HIF-1 伪 on PD-L1 in lung adenocarcinoma. Methods the expression of HIF-1 伪 PD-L1 in 99 cases of lung adenocarcinoma was detected by immunohistochemical method. The expression of PD-L1 in 99 cases of lung adenocarcinoma was treated with simulated chemical hypoxia. It was divided into two groups: normal group and CoCl_2 group. The expression of HIF-1 伪 -PD-L1 mRNA was detected by qRT-PCR. Western blotting was used to detect the expression of HIF-1 伪 -pPD-L1 protein. SiRNA-HIF-1 伪 was transiently transfected into lung adenocarcinoma cells. The efficiency of transfection and the expression of PD-L1 mRNA and protein in 99 cases of lung adenocarcinoma were detected by Western blotting. Results the positive rates of HIF-1 伪 and PD-L1 in 99 cases of lung adenocarcinoma were 47.48% and 34.34%, respectively, and the expression levels of HIF-1 伪 were correlated with the age and clinical stage of the patients. There was a positive correlation between the expression of P0.05HIF-1 伪 protein and the expression of PD-L1 protein. Compared with the normal group, the expression level of HIF-1 伪 protein in the cells of CoCl2 group was significantly higher than that in the normal group. The expression level of PD-L1 mRNA and protein increased significantly. Compared with control group, the expression of PD-L1 mRNA and protein in the silenced cells of the negative control group was significantly lower than that in the control group. Conclusion the expression of HIF-1 伪 and PD-L1 in lung adenocarcinoma is regulated by HIF-1 伪.
【作者单位】：山东大学附属省立医院检验部;
【基金】：国家自然科学基金(81372775) 山东省医药卫生科技发展计划(2014WS0076)
【分类号】：R734.2

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