miR-218调控SNX4蛋白对乳腺癌细胞增殖和侵袭的影响

发布时间：2018-02-24 12:01

本文关键词： 乳腺癌 miR- SNX 侵袭　出处：《中国免疫学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的:探讨miR-218调控SNX4蛋白对乳腺癌细胞增殖和侵袭能力的影响。方法:q PCR检测乳腺癌组织和乳腺癌细胞株中miR-218的表达情况;分析miR-218的表达和乳腺癌临床病理参数之间的关系;双荧光素酶实验检测miR-218和SNX4之间的关系;过表达miR-218后MTT实验和侵袭实验检测乳腺癌细胞增殖和侵袭能力变化;过表达SNX4后MTT实验和侵袭实验检测SNX4对乳腺癌细胞增殖和侵袭能力的恢复水平;裸鼠体外成瘤实验检测miR-218对乳腺癌细胞株成瘤能力的影响。结果:miR-218在乳腺癌组织和MCF-7细胞株中表达水平较高,miR-218的表达与乳腺癌的病理分期相关以及淋巴结转移情况有关,SNX4可能是miR-218下游的作用靶点;过表达miR-21可以抑制乳腺癌细胞的增殖侵袭能力,过表达SNX4后可以逆转miR-218对乳腺癌细胞的抑制作用,过表达miR-218后可以抑制乳腺癌细胞株在裸鼠体内的成瘤能力。结论:miR-218在乳腺癌中表达上调,同时miR-218可以调控SNX4的表达而影响乳腺癌细胞增殖和侵袭能力。
[Abstract]:Objective: to investigate the effect of SNX4 protein regulated by miR-218 on the proliferation and invasion of breast cancer cells. Methods: the expression of miR-218 in breast cancer tissues and breast cancer cell lines was detected by PCR, and the relationship between the expression of miR-218 and the clinicopathological parameters of breast cancer was analyzed. The relationship between miR-218 and SNX4 was detected by double luciferase assay, and the changes of proliferation and invasion ability of breast cancer cells were detected by MTT assay and invasion assay after overexpression of miR-218. After overexpression of SNX4, MTT assay and invasion assay were used to detect the recovery of SNX4 to the proliferation and invasion of breast cancer cells. In vitro tumorigenesis assay in nude mice was used to detect the effect of miR-218 on the tumorigenic ability of breast cancer cell lines. Results the high expression of miR-218 in breast cancer tissues and MCF-7 cell lines was related to the pathological stage of breast cancer and lymph node metastasis. SNX4 may be the target of miR-218 downstream. Overexpression of miR-21 can inhibit the proliferation and invasion of breast cancer cells, and overexpression of SNX4 can reverse the inhibitory effect of miR-218 on breast cancer cells. Overexpression of miR-218 can inhibit the tumorigenic ability of breast cancer cell line in nude mice. Conclusion: miR-218 can up-regulate the expression of SNX4 in breast cancer, while miR-218 can regulate the expression of SNX4 and affect the proliferation and invasion of breast cancer cells.
【作者单位】：郑州大学附属肿瘤医院病理科;
【基金】：河南省基础与前沿技术研究计划基金(No.102300410038)
【分类号】：R737.9

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