人胎盘提取物抑制结肠癌细胞的增殖及迁移

发布时间：2018-02-25 15:57

本文关键词： 结肠癌人胎盘提取物 Caco-细胞 SW细胞细胞凋亡细胞周期　出处：《中国肿瘤生物治疗杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的:探讨人胎盘提取物(human placenta extracts,HPE)对人结肠癌细胞株Caco-2及SW480增殖和迁移能力的影响。方法:用不同质量浓度HPE处理Caco-2和SW480细胞,CCK-8、Ki-67、CFSE法检测细胞增殖活性的变化,Annexin-V/PI及DAPI细胞核染色检测HPE对细胞周期及凋亡的影响,实时荧光定量PCR法检测HPE对细胞中BAX、CDK2及Cyclin A2基因表达的影响,划痕实验检测对细胞迁移能力的影响。建立裸鼠结肠癌移植瘤模型,随机分组分别单独或联合给予HPE和5-氟尿嘧啶(5-Fu),观察裸鼠移植瘤的生长状况。结果:HPE处理组Caco-2及SW480细胞增殖能力低于对照组[(0.82±0.01)vs(0.96±0.02),P0.01;(0.90±0.03)vs(0.96±0.02),P0.05],细胞凋亡率高于对照组[(20.47±1.32)%vs(11.01±3.82)%,P0.01;(20.70±5.19)%vs(8.00±2.69)%,P0.05];HPE处理组细胞胞质减少、核固缩、BAX基因表达增加[(3.23±1.90)vs(1.00±0.00),(2.25±0.55)vs(1.00±0.00),均P0.05];细胞停留在细胞DNA复制S期,出现凋亡峰;Caco-2细胞CDK2及Cyclin A2基因表达降低(P0.05);并且细胞的迁移能力低于对照组[(0.17±0.29)vs(1.50±0.50)mm,P0.05]。HPE处理组裸鼠移植瘤体积小于对照组、生存率高于单用5-Fu组(P0.05)。结论:HPE在体内外通过干预结肠癌细胞凋亡、阻滞细胞周期、抑制细胞增殖及迁移,可能对抑制肿瘤细胞增殖有一定作用。
[Abstract]:Objective: to investigate the effect of human placental extract (HPE) on the proliferation and migration of human colon cancer cell line Caco-2 and SW480. Methods: the proliferation activity of Caco-2 and SW480 cells treated with different concentrations of HPE was detected by using the method of CCK-8 Ki-67CFSE. The effect of HPE on cell cycle and apoptosis was detected by nuclear staining. The effect of HPE on the expression of BAXT CDK2 and Cyclin A2 genes was detected by real-time fluorescence quantitative PCR assay, and the effect of scratch test on cell migration was detected. The growth status of transplanted tumor was observed in nude mice treated with HPE alone or in combination with 5-FU. Results the proliferative ability of Caco-2 and SW480 cells in the treated group was lower than that in the control group [0.82 卤0.01 vs 0.96 卤0.02 卤0.03 vs 0.96 卤0.03 vs 0.96 卤0.02], and the apoptotic rate was higher than that in the control group [20.47 卤1.32vs11.01 卤3.82VS 11.01 卤3.82VS = 20.70 卤5.19vs8.00 卤2.69p05] the cell cytoplasm of HPE-treated group was decreased. The expression of Bax gene was increased [3.23 卤1.90 vs 1.00 卤0.55 卤0.55 vs 1.00 卤0.005, P 0.05], and the expression of CDK2 and Cyclin A2 in Caco-2 cells was decreased in S phase of cell DNA replication, and the migration ability was lower than that in control group [0.17 卤0.29 vs 1.50 卤0.50 卤0.50mm P 0.05]. The transplanted tumor volume in HPE treated group was lower than that in control group, and the expression of CDK2 and Cyclin A2 gene in Caco-2 cells was lower than that in control group, and the expression of Cyclin A2 gene in Caco-2 cells was lower than that in control group (P < 0.05), and the migration ability of the cells was lower than that of the control group (P < 0.05). The survival rate of 5-Fu group was higher than that of 5-Fu group. Conclusion in vivo and in vitro, by interfering with apoptosis of colon cancer cells, blocking cell cycle, inhibiting cell proliferation and migration, WHPE may play a certain role in inhibiting tumor cell proliferation.
【作者单位】：贵州医科大学研究生院;贵州医科大学附属医院临床医学研究中心;贵州医科大学附属医院微生物免疫科;
【基金】：国家自然科学基金资助项目(No.81560266) 贵州医科大学附属医院博士启动基金资助项目~~
【分类号】：R735.35

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