Survivin与Klotho对胃肠道神经内分泌癌预后的影响

发布时间：2018-02-27 16:30

  本文关键词： Klotho Survivin 胃肠道神经内分泌癌　出处：《南华大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:检测Survivin和Klotho在胃肠道神经内分泌癌患者癌组织及癌旁组织中表达,探讨Survivin和Klotho与胃肠道神经内分泌癌患者的预后的相关性。方法:对51例胃肠道神经内分泌癌患者的病例资料进行回顾性研究,运用免疫组化和Western blot法来检测GNT患者癌组织及癌旁组织Survivin和Klotho的表达;分析和研究两种蛋白质的表达与胃肠道神经内分泌癌患者预后的相关性。结果:1.在51例胃肠道神经内分泌癌患者中有35例(68.63%)患者癌组织Survivin阳性,癌旁组织中未发现有Survivin的表达。运用免疫组化、Western Blot检测Survivi在GNT癌组织和癌旁组织中的表达情况。免疫组化结果显示小细胞癌中Survivin的表达强度和数量(18/21)高于大细胞癌(14/30),癌旁组织中Survivin表达两者无差异。临床资料发现Survivin的表达与肿瘤分期和组织分化相关,与年龄、性别等其他因素无关。分析显示低分化及未分化的癌组织中Survivin的表达显著高于高分化癌(P=0.0013),其中在临床分期Ⅲ期(5/7)、Ⅳ期(12/12)中Survivin的阳性表达率为100%,显著高于Ⅱ期(15/27)Ⅰ期(1/5)期癌组织中Survivin的表达(P=0.0023)。2.51例肿瘤标本免疫组化检测Klotho发现所有标本癌旁组织Klotho的表达均呈强阳性,21例标本的胃肠道神经内分泌癌组织Klotho呈现弱阳性反应;Western Blot检测Klotho发现胃肠道神经内分泌癌旁组织中的Klotho的表达显著高于癌组织。Klotho的表达与组织分化和肿瘤分期相关,与年龄等其他因素无关。Ⅳ期的患者中没有检测到Klotho的表达,高分化者(6/7)的癌组织中Klotho的表达显著高于低分化、未分化者(0/6,0/4,P=0.0035),在Ⅰ期患者的Klotho的阳性表达率为(5/5)显著高于Ⅱ(13/27)和Ⅲ(2/7)期患者(P=0.0067)。结论:1.胃肠道神经内分泌癌患者中,Survivin阳性表达小细胞癌显著高于大细胞癌,大细胞癌中显著高于癌旁组织;2.胃肠道神经内分泌癌患者中,Klotho阳性表达小细胞癌显著低于大细胞癌,大细胞癌明显低于癌旁组织;3.Survivin和Klotho的表达与胃肠道神经内分泌癌患者组织分化、肿瘤分期相关,两者是预测和评估胃肠道神经内分泌癌患者预后的重要指标。
[Abstract]:Objective: to detect the expression of Survivin and Klotho in cancer tissues and adjacent tissues of patients with gastrointestinal neuroendocrine carcinoma. To investigate the relationship between Survivin and Klotho and the prognosis of patients with gastrointestinal neuroendocrine carcinoma. Methods: the data of 51 patients with gastrointestinal neuroendocrine carcinoma were retrospectively studied. Immunohistochemical and Western blot methods were used to detect the expression of Survivin and Klotho in cancer tissues and adjacent tissues of GNT patients. The relationship between the expression of two proteins and the prognosis of patients with gastrointestinal neuroendocrine carcinoma was analyzed and studied. Results: Survivin was positive in 35 out of 51 patients with gastrointestinal neuroendocrine carcinoma. No expression of Survivin was found in the paracancerous tissues. The expression of Survivi in GNT and adjacent tissues was detected by immunohistochemical Blot. The expression of Survivin in small cell carcinoma was higher than that in large cell. The expression intensity and quantity of Survivin in small cell carcinoma were higher than that in large cell. There was no difference between the expression of Survivin in paracancerous tissues. Clinical data showed that the expression of Survivin was related to tumor stage and tissue differentiation. With age, Analysis showed that the expression of Survivin in poorly differentiated and undifferentiated cancer tissues was significantly higher than that in well-differentiated carcinoma tissues. The positive expression rate of Survivin in clinical stage 鈪，

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