细胞型朊蛋白（PrP~C）在直肠癌中的表达及临床意义

发布时间：2018-02-27 23:09

  本文关键词： 细胞型朊蛋白 直肠癌 直肠腺瘤 免疫组织化学 肿瘤标志物　出处：《南昌大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的:检测直肠癌组织、直肠腺瘤组织及相应癌旁正常直肠组织中细胞型朊蛋白(PrPC)的表达含量,分析PrPC在直肠癌中表达水平与肿瘤分化、临床分期及淋巴结转移等临床病理参数之间的相关性,初步探讨其在直肠癌发生发展中的临床意义,以期为阐明直肠癌的发病机制提供理论依据。方法:收集南昌大学第一附属医院胃肠外科2013年6月至2014年4月手术切除后经病理证实为直肠癌的石蜡包埋组织标本60例,并取相应癌旁直肠组织和手术切除后经病理诊断为直肠腺瘤组织标本各20例作为对照组,采用链霉菌抗生物素蛋白-过氧化物酶连结法(streptavidin-perosidase,SP法)检测直肠癌组织、直肠腺瘤组织及癌旁正常直肠组织中PrPC的表达情况,分别统计各组PrPC阳性表达率,应用统计分析软件SPSS19.0分析处理实验数据。结果:PrPC在直肠癌组织中的阳性表达率最高,为65%(39/60),高于直肠腺瘤组的35%(7/20)及癌旁正常直肠组的15%(3/20)(P0.05),但直肠腺瘤组与癌旁正常直肠组PrPC阳性表达率比较差异无显著性(P0.05),肿瘤分化程度为中/高分化组PrPC阳性率为54.76%(23/42),肿瘤分化程度为低分化组PrPC阳性表达率为88.89%(16/18),两组差异比较具有统计学意义(P0.05);TNM分期为I-II期PrPC阳性表达率为45.45%(10/22),TNM分期为III-IV期PrPC阳性表达率为76.32%(29/38),两组PrPC阳性表达率比较差异有统计学意义(P0.05);直肠癌淋巴结有转移组PrPC阳性表达率为77.78%(28/36),无淋巴结转移组PrPC阳性表达率为45.83%(11/24),两组比较差异有统计学意义(P0.05);然而PrPC在不同性别、年龄、肿瘤位置及肿瘤大小中的表达差异均无统计学意义(P0.05)。结论:PrPC在直肠癌组织中的表达高于直肠腺癌及癌旁直肠组织中的表达,提示PrPC与直肠癌的发生发展密切相关;PrPC的表达与直肠癌的分化程度、TNM分期及淋巴结转移显著相关,提示PrPC可能参与了直肠癌的发展、浸润及转移。
[Abstract]:Objective: to detect the expression of cellular prion protein (PrPC) in rectal cancer tissues, rectal adenoma tissues and adjacent normal rectal tissues, and to analyze the expression level and differentiation of PrPC in rectal cancer. To explore the clinical significance of clinical staging and lymph node metastasis in the occurrence and development of rectal cancer. Methods: from June 2013 to April 2014, 60 cases of paraffin embedded tissues of rectal cancer proved by pathology were collected from gastrointestinal surgery of the first affiliated Hospital of Nanchang University. The rectal tissues adjacent to cancer and 20 cases of rectal adenoma diagnosed by pathology after resection were taken as the control group. Streptavidin-perosidase SP method was used to detect rectal cancer tissue by streptavidin-perosidase (SP) method. The expression of PrPC in rectal adenoma and adjacent normal rectal tissues was analyzed. The positive expression rate of PrPC in each group was counted, and the experimental data were analyzed by SPSS19.0 software. Results the positive rate of PrPC was the highest in rectal cancer. It was 65 / 39 / 60, higher than 35 / 7 / 20 in rectal adenoma and 15 / 3 / 20 / 20 in normal rectum adjacent to carcinoma, but there was no significant difference in PrPC positive rate between rectal adenoma group and paracancerous normal rectum group. The positive rate of PrPC was 54.76% / 2342% in middle / high differentiation group, but there was no significant difference between rectal adenoma group and paracancerous normal rectum group (P 0.05). The positive expression rate of PrPC in the low differentiation group was 88.89 / 18 / 18. The difference between the two groups was statistically significant. The positive expression rate of PrPC in I-II stage was 45.4545 / 22. The positive expression rate of PrPC in III-IV stage was 76.32 / 29 / 3838, and the difference between the two groups was significant. The positive expression rate of PrPC in lymph node metastasis group of rectal cancer was 77.78% / 36%, and the positive rate of PrPC expression in non-lymph node metastasis group was 45.83% / 24%. The difference between the two groups was statistically significant (P 0.05). However, PrPC was expressed in different genders. There was no significant difference in age, tumor location and tumor size. Conclusion the expression of PrPC in rectal cancer is higher than that in rectal adenocarcinoma and adjacent rectal tissue. The results suggest that the expression of PrPC is closely related to the occurrence and development of rectal cancer, and the expression of PrPC is significantly correlated with the differentiation and lymph node metastasis of rectal cancer, suggesting that PrPC may be involved in the development, invasion and metastasis of rectal cancer.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.37

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