口腔鳞状细胞癌中Pinx1的表达情况及意义

发布时间：2018-03-03 01:22

本文选题：Pinx1　切入点：口腔鳞状细胞癌　出处：《吉林大学》2017年硕士论文　论文类型：学位论文

【摘要】：研究背景及意义:口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)是头颈颌面部发病率较高的恶性肿瘤之一,在成年人中多发,以中老年男性多见,常多发于舌、颊、腭等部位,其晚期常发生淋巴结转移,预后较差,易造成患者治疗失败甚至死亡。研究证实,促癌基因和抑癌基因对于口腔鳞癌的发生、发展有十分重要的作用,近年来得到广泛关注。端粒是位于染色体末端的特殊DNA序列,是细胞中维持基因组稳定性的重要组成部分。端粒酶维持端粒长度和结构,端粒酶活性过强将导致细胞不能正常衰老死亡,成为癌细胞。Pinx1(PIN2码/TRF1相互作用蛋白X1)是内源性端粒酶抑制基因,位于染色体8p23区域,由7个外显子组成,能降低端粒酶酶促反应活性,缩短端粒长度,促使肿瘤细胞程序性凋亡。研究发现,在肿瘤组织中呈低表达或不表达的Pinx1基因在人体正常组织细胞中呈高表达,提示Pinx1与癌瘤的发生和发展有一定关系,Pinx1有望成为相关肿瘤诊断和治疗的分子标志物以及分子靶向治疗的有效蛋白因子。目前,国内外尚未见Pinx1在口腔鳞状细胞癌中表达情况的报道。实验目的:研究Pinx1基因在正常口腔黏膜上皮组织及不同分化程度、伴或不伴有淋巴结转移的口腔鳞癌组织中的表达情况及意义,探讨Pinx1的表达水平与口腔鳞癌分化程度及有无淋巴结转移之间的关系,从基因分子水平分析该基因对口腔鳞癌病理诊断及临床治疗的意义和作用。实验方法:本实验选取60例口腔鳞癌组织(高、中、低分化鳞癌组织各20例)和20例正常口腔黏膜组织应用免疫组织化学染色(Immun ohistochemistry,IHC)的方法进行染色分析,检测Pinx1在不同组织中的表达情况,结果采用SPSS19.0软件进行统计学分析,并运用c2检验对比分析各组间Pinx1的表达水平,以P0.05作为具有统计学差别的衡量标准,从而探索Pinx1基因的表达水平与口腔鳞癌分化水平及淋巴结转移之间是否具有相关性。实验结果:1.Pinx1在所有正常口腔黏膜上皮中均呈高表达,在口腔鳞癌中表达水平较正常口腔黏膜低(P0.05)。2.随着口腔鳞癌组织分化程度的降低,Pinx1的表达程度呈降低趋势(P0.05)。3.伴有淋巴结转移的口腔鳞癌切片中Pinx1表达水平低于不伴有淋巴结转移者,二者具有显著差异性(P0.05)。4.Pinx1的表达情况与患者性别年龄无显著相关性(P0.05)。实验结论:由实验结果可以看出,Pinx1的表达与年龄及性别无关。Pinx1在口腔鳞癌组织中的表达水平明显低于正常口腔黏膜上皮。随着肿瘤分化程度降低及出现淋巴结转移,Pinx1的表达水平也降低。由此提示Pinx1的表达与口腔鳞癌的发生及发展关系密切,Pinx1有望成为诊断口腔鳞癌、判别口腔鳞癌恶变程度的分子标志物,并有可能成为其基因治疗的重要靶基因。
[Abstract]:Background and significance: oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors in head, neck and maxillofacial region. Lymph node metastasis often occurs in the late stage, and the prognosis is poor. It is easy to cause failure or even death of the patients. Studies have proved that the oncogenes and tumor suppressor genes play a very important role in the development and development of oral squamous cell carcinoma (OSCC). In recent years, telomere is a special DNA sequence located at the end of chromosome, which is an important part of maintaining genomic stability in cells. Telomerase maintains telomere length and structure. Overexpression of telomerase activity leads to cell aging and death. It is an endogenous telomerase suppressor gene, which is composed of seven exons, located in chromosome 8p23, and becoming cancer cell. Pinx1PIN2 code / TRF1 interaction protein X1 is an endogenous telomerase suppressor gene. It can reduce telomerase activity, shorten telomere length and promote programmed apoptosis of tumor cells. It has been found that Pinx1 gene, which is low or not expressed in tumor tissues, is highly expressed in human normal tissues. It is suggested that Pinx1 may be a molecular marker for the diagnosis and treatment of cancer and an effective protein factor for molecular targeted therapy. The expression of Pinx1 in oral squamous cell carcinoma has not been reported at home and abroad. The expression and significance of Pinx1 in oral squamous cell carcinoma (OSCC) with or without lymph node metastasis, and to explore the relationship between the expression of Pinx1 and the degree of differentiation and lymph node metastasis in oral squamous cell carcinoma (OSCC). The significance and effect of the gene on the pathological diagnosis and clinical treatment of oral squamous cell carcinoma were analyzed at the molecular level. 20 cases of poorly differentiated squamous cell carcinoma (20 cases each) and 20 cases of normal oral mucosa were analyzed by immunohistochemical staining method to detect the expression of Pinx1 in different tissues. The results were statistically analyzed by SPSS19.0 software. The expression level of Pinx1 in each group was analyzed by c2 test, and P05 was taken as the standard of statistical difference. In order to explore the correlation between the expression level of Pinx1 gene and the differentiation level and lymph node metastasis of oral squamous cell carcinoma, the results showed that: 1. Pinx1 was highly expressed in all normal oral mucosal epithelium. In oral squamous cell carcinoma (OSCC), the expression level was lower than that in normal oral mucosa (P 0.05). 2. With the decrease of differentiation degree of oral squamous cell carcinoma (OSCC), the expression of Pinx1 decreased. The expression level of Pinx1 in OSCC with lymph node metastasis was lower than that in OSCC without lymph node metastasis. Those with lymph node metastasis, There was no significant correlation between the expression of Pinx1 and sex and age of patients. Conclusion: from the experimental results, we can see that the expression of Pinx1 is not related to age and sex. The expression level of Pinx1 in oral squamous cell carcinoma is clear. The expression of Pinx1 is lower than that of normal oral mucosal epithelium. With the decrease of tumor differentiation and lymph node metastasis, the expression of Pinx1 is closely related to the occurrence and development of oral squamous cell carcinoma (OSCC). Molecular markers for judging the degree of malignancy in oral squamous cell carcinoma (OSCC) may be an important target gene for gene therapy of oral squamous cell carcinoma (OSCC).
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.8

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