长链非编码RNA GAS5在子宫内膜癌中的表达及对肿瘤侵袭的影响

发布时间：2018-03-03 11:04

  本文选题：lnc　切入点：RNA-GAS　出处：《中国现代医学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的探讨长链非编码RNA GAS5在子宫内膜癌组织中的表达及其对子宫内膜癌HEC-1A细胞侵袭能力的影响。方法筛选2013年1月-2015年12月间于解放军451医院妇产科行手术切除的子宫内膜癌患者的癌及癌旁组织50例,分析lncRNA-GAS5在组织中的表达水平及临床意义;通过重组质粒在人子宫内膜癌HEC-1A细胞中过表达lncRNA-GAS5,采用Transwell侵袭小室模型检测过表达lncRNA-GAS5后HEC-1A细胞侵袭能力变化,qRT-PCR检测micro RNA-21的表达变化,qRT-PCR及Western blot检测PTEN表达变化。结果 lncRNA-GAS5在子宫内膜癌组织中表达水平低于对应癌旁组织(t=4.013,P0.05),子宫内膜癌组织中长链非编码核醣核酸生长组织特异性转录体5(lncRNA-GAS5)低表达与肿瘤淋巴结转移(t=2.389,P=0.017)及高FIGO分期(Ⅲ+Ⅳ期,t=2.131,P=0.021)差异有统计学意义;lncRNA-GAS5重组质粒转染HEC-1A细胞后可抑制细胞的侵袭能力(t=7.654,P=0.008);qRT-PCR检测发现过表达lncRNA-GAS5后microRNA-21(miR-21)的表达水平升高(t=23.533,P0.05),qRT-PCR及Western blot结果证实,过表达lncRNA-GAS5后可促进PTEN的表达(P0.05)。结论 lncRNA-GAS5在子宫内膜癌组织中表达降低,lncRNA-GAS5可能通过下调miR-21的表达进而恢复PTEN的表达来抑制子宫内膜癌转移。
[Abstract]:Objective to investigate the expression of long chain noncoding RNA GAS5 in endometrial carcinoma and its effect on the invasiveness of endometrial carcinoma HEC-1A cells. Methods from January 2013 to December 2015, surgical excision of gynecology and obstetrics in 451 Hospital of PLA was performed. 50 cases of endometrial carcinoma and adjacent tissues, To analyze the expression level and clinical significance of lncRNA-GAS5 in tissues. The expression of micro RNA-21 in human endometrial carcinoma HEC-1A cells was detected by qRT-PCR and Western blot was used to detect the expression of PTEN by using Transwell invasive chamber model to detect the changes of invasion ability of HEC-1A cells after overexpression of lncRNA-GAS5. The expression level of lncRNA-GAS5 in endometrial carcinoma was lower than that in corresponding adjacent tissues (P 0.05). The low expression of long chain non-coding RNA specific transcripts (5lncRNA-GAS5) and lymph node metastasis in endometrial carcinoma tissues were lower than those in corresponding adjacent tissues (P 0.017) and high FIGO stage (P < 0.05). There was significant difference in the invasion ability of HEC-1A cells after transfection of recombinant plasmid LncRNA-GAS5. The expression level of microRNA-21mmiR-21 after lncRNA-GAS5 expression was detected by RT-PCR. The results of Western blot and microRNA-21mmiR-21 showed that the expression level of microRNA-21 mRNA was increased after transfection of GAS5 plasmid into HEC-1A cells. Overexpression of lncRNA-GAS5 can promote the expression of PTEN P0.05.Conclusion the decrease of lncRNA-GAS5 expression in endometrial carcinoma may inhibit the metastasis of endometrial carcinoma by down-regulating the expression of miR-21 and restoring the expression of PTEN.
【作者单位】： 解放军451医院妇产科;解放军451医院腔镜外科;
【分类号】：R737.33
，

本文编号：1560760