CD88和COX-2在肝细胞肝癌中表达的相关性及其意义

发布时间：2018-03-05 16:23

本文选题：肝细胞肝癌　切入点：COX-2　出处：《新乡医学院》2015年硕士论文　论文类型：学位论文

【摘要】：目的目前,癌症已经成为人类健康的第一杀手,尤其是肝细胞肝癌(Hepatocellular carcinoma, HCC),在中国甚至世界范围内,发病率和死亡率在逐年升高。因此,寻找新的诊断和治疗相关分子协助临床医生快速有效地诊断和治疗HCC将有利于提高HCC病人的疗效和生存率。环氧合酶(cyclooxygenase, COX)在体内有着重要的作用,近年来的研究表明,Ⅱ型环氧合酶(COX-2)在食管癌、肝癌中表达增高,与肿瘤血管的形成密切相关。CD88又称补体5片段受体(complement component 5a receptor, C5aR),常被用来作为鉴定白细胞系的标志,与C5a结合后能够激活PI3K/A KT/mTOR和IL-6/STAT3两个信号通路并促进肝细胞的分裂增殖和对抗凋亡,趋化白细胞和参与炎症反应,在肿瘤侵袭转移中发挥重要作用。已有研究显示CD88可能促进肿瘤周围血管新生,加速肿瘤细胞侵袭转移。由于COX-2和CD88在肿瘤组织中的表达均显著增高,并且与肿瘤的侵袭转移密切相关,因此,本研究将探讨二者在肝细胞肝癌组织中的表达率以及二者之间的相关性。方法1.标本收集收集新乡市第一人民医院病理科2013年1月至2014年4月期间,所有资料完整的肝细胞肝癌组织共57例。根据年龄、性别、肿瘤直径、分化程度、分期和有无淋巴结转移等将标本分类。一部分组织放入液氮保存,另一部分组织经10%的福尔马林溶液固定、石蜡包埋以及4gm厚度连续切片。2. RT-PCR检测正常肝组织和肝细胞肝癌组织中COX-2和CD88mRNA的表达提取肝正常组织和肝细胞肝细胞肝癌组织中总mRNA,检测组织中COX-2和CD88 mRNA的表达情况。3.免疫组织化学染色观察COX-2和CD88蛋白的表达将准备好的组织切片用SABC法进行免疫组织化学染色,观察并确定COX-2和CD88的表达部位,计算二者的表达率以及表达的相关性。4.结果判断RT-PCR的结果用Image J软件测量灰度值,根据与内参的对比,判断COX-2和CD88 mRNA的表达。免疫组织化学结果根据染色强度和阳性细胞所占的百分比分别计分,再将染色强度分值与阳性细胞所占百分比分值相乘所得乘积判定染色结果。5.统计学处理计量数据均使用均数±标准差(x±s)表示,应用SPSS 17.0统计软件对数据进行统计学处理。多样本均数的比较采用方差分析；阳性率的比较用χ2检验；相关性分析用Pearson检验,检验水准均设为α=0.05,即P0.05有显著统计学差异,P0.01有非常显著统计学差异,P0.05无统计学差异。结果1.COX-2和CD88 mRNA的表达COX-2和CD88 mRNA在正常肝组织和肝细胞肝癌组织中均有表达,且在肝细胞肝癌组织的表达较正常肝组织明显增高。二者在低、中、高分化癌组织中表达逐渐增高,低、中分化组表达无统计学差异,而二者与高分化组相比有统计学差异(P0.05)。2.COX-2在肝细胞癌中的表达及与临床病理特征的关系COX-2染色阳性的细胞其细胞浆中呈现弥漫性分布的黄色和棕黄色颗粒状物质,肝细胞癌组织中的COX-2阳性率为73.68%。COX-2蛋白的表达与肿瘤分化程度、肿瘤大小、临床分期、淋巴结转移情况无关(P0.05)。3.CD88在肝细胞癌中的表达及与临床病理特征的关系CD88蛋白表达主要位于细胞膜,部分位于胞浆中。肝细胞癌组织中的CD88蛋白表达的阳性率为82.45%。CD88蛋白的表达与肿瘤分化程度、肿瘤大小、临床分期、淋巴结转移情况无关(P0.05)3.COX-2与CD88在肝细胞癌中的表达的相关性肝癌组织中,COX-2阳性组,CD88的阳性率为90.48%,显著高于COX-2阴性组(60%),COX-2和CD88的蛋白表达呈正相关(r=0.353,P0.05)。结论1.肝细胞肝癌组织中COX-2和CD88 mRNA的表达较正常肝组织明显增多,但COX-2和CD88蛋白的表达与肿瘤分化程度、肿瘤大小、临床分期和淋巴结转移均无关。2.肝细胞肝癌组织中COX-2和CD88蛋白的表达呈正相关。
[Abstract]:At present, cancer has become the first killer of human health, especially hepatocellular carcinoma (Hepatocellular, carcinoma, HCC) in China even in the world, the morbidity and mortality increased year by year. Therefore, finding new molecular diagnosis and treatment to assist clinicians effectively in diagnosis and treatment of HCC will improve HCC patients curative effect and survival rate. Cyclooxygenase (cyclooxygenase, COX) plays an important role in the body, recent studies show that cyclooxygenase-2 (COX-2) expression in esophageal cancer, liver cancer, and tumor angiogenesis is closely related to the.CD88 also known as complement 5 receptor (complement component fragment 5A receptor, C5aR), marks are often used as identification of white blood cells, when combined with C5a can activate PI3K/A KT/mTOR and IL-6/STAT3 two signaling pathway and promote liver cell proliferation and apoptosis resistance Dead, chemotaxis of white blood cells and participate in inflammation, play an important role in tumor invasion and metastasis. Studies have shown that CD88 may promote tumor angiogenesis, accelerate tumor cells metastasis. The expression of COX-2 and CD88 in tumor tissues were significantly increased, and the tumor invasion and metastasis are closely related, therefore, the the research will explore the correlation between the expression rate of two in hepatocellular carcinoma and two. Methods: 1. tissue samples collected from Xinxiang First People's Hospital Department of pathology from January 2013 to April 2014, all data of hepatocellular carcinoma in 57 cases. According to age, gender, tumor size, degree of differentiation, staging and lymph node metastasis as part of the organization classification. Specimens were preserved in liquid nitrogen, the other part of the organization in 10% formalin fixed, paraffin embedded and 4gm continuous.2. R slice thickness The expression of COX-2 and CD88mRNA T-PCR detection of normal liver tissue and hepatocellular carcinoma total mRNA was extracted from normal liver tissue and liver cells in hepatocellular carcinoma, the expression of.3. COX-2 and CD88 immunohistochemical staining in detection of mRNA expression of COX-2 and CD88 proteins will be prepared by the method of SABC immune tissue histochemical staining, observe and determine the expression site of COX-2 and CD88. The correlation between the expression of.4. two and the expression rate of calculation results to determine the results of RT-PCR using Image J software to measure the gray value, according to the comparison and reference, the expression of COX-2 and CD88 mRNA found fault. According to the results of immunohistochemical staining intensity and the percentage of positive cells for the score, the staining intensity score and the percentage of the positive cells were obtained by multiplying the product to determine the staining results statistically.5. measurement data are made With the mean and standard deviation (x + s), using SPSS 17 statistical software for statistical processing of data. Compared with the mean diversity analysis of variance; the positive rate of the 2 test was used to compare; correlation analysis with Pearson test, the test level for alpha =0.05, P0.05 had significant difference P0.01, there are very significant differences, no significant difference P0.05. Results the expression of COX-2 and CD88 mRNA 1.COX-2 and CD88 mRNA in normal liver tissue and hepatocellular carcinoma tissues, and the expression in hepatocellular carcinoma tissues than in normal liver tissue were significantly increased. Two in the low and high differentiation cancer tissues the expression gradually increased in low differentiation group, there were no significant difference, but the two compared with the high differentiation group had significant difference (P0.05) expression of.2.COX-2 in hepatocellular carcinoma and its relationship with clinical pathological characteristics of COX-2 positive cells The cytoplasm showed diffuse distribution of yellow and brown granular material, the positive rate of COX-2 in hepatocellular carcinoma 73.68%.COX-2 protein expression and tumor differentiation, tumor size, clinical staging, independent of lymph node metastasis (P0.05) expression of.3.CD88 in hepatocellular carcinoma and its relationship with clinical pathological characteristics CD88 protein was mainly located in the cell membrane, located in the cytoplasm. The positive expression rate of hepatocellular carcinoma CD88 protein 82.45%.CD88 protein expression and tumor differentiation, tumor size, clinical stage, lymph node metastasis (P0.05). The expression of 3.COX-2 and CD88 in hepatocellular carcinoma associated hepatocellular carcinoma in COX-2 positive group, the positive rate of CD88 was 90.48%, significantly higher than that of COX-2 negative group (60%), COX-2 and CD88 protein expression were positively correlated (r=0.353, P0.05) COX-2 and CD 1.. Conclusion liver cells in hepatocellular carcinoma 88 mRNA expression increased significantly compared with normal liver tissue, but the expression of COX-2 and CD88 protein was not related to tumor differentiation, tumor size, clinical stage and lymph node metastasis. The expression of COX-2 and CD88 protein in.2. HCC tissues was positively correlated.

【学位授予单位】：新乡医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.7

【相似文献】