PEDF在食管鳞状细胞癌中的表达及临床意义

发布时间：2018-03-05 18:33

本文选题：食管鳞状细胞癌　切入点：PEDF　出处：《川北医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:研究色素上皮衍生因子(Pigment epithelium-derived factor,PEDF)在食管鳞状细胞癌组织中的表达及其临床意义,探讨食管鳞状细胞癌PEDF蛋白表达情况与微血管密度(MVD)的相关性。方法:取2016年2月至2016年10月四川省南充市中心医院和川北医学院附属医院心胸外科手术切除的食管鳞状细胞癌组织及周围正常食管组织64例,所有患者术前均未接受放疗、化疗和其他抗肿瘤药物的干预。应用实时荧光定量PCR(real-time quantitative PCR,RT-PCR)检测64例食管鳞状细胞癌组织和同一患者周围正常食管组织中PEDF mRNA表达,应用免疫组化检测64例食管鳞状细胞癌组织和同一患者周围正常食管组织中PEDF蛋白表达及癌组织中MVD表达水平。结果:实时荧光定量PCR结果表明:64对实验组织标本中PEDF mRNA均有不同强度的表达,其中食管鳞状细胞癌组织中PEDF mRNA相对定量值为0.77,比配对的周围正常食管组织(1.04)明显下降,两者之间比较有统计学差异(P0.05)。免疫组化检测结果表明:(1)PEDF蛋白的表达情况:64例食管鳞状细胞癌组织标本中29例PEDF蛋白表达阳性,35例PEDF蛋白表达阴性,阳性表达率为45.31%,而在正常食管组织标本中43例PEDF蛋白表达阳性,21例PEDF蛋白表达阴性,阳性表达率为67.19%,两组之间表达差异有统计学意义(P0.05)。食管鳞状细胞癌组织中PEDF蛋白表达与患者的年龄、性别无明显相关(P0.05),而与肿瘤的临床分期、组织分化程度和淋巴结转移有关(P0.05),且肿瘤组织分化程度越低、分期越晚或有淋巴结转移者,PEDF蛋白表达越低。(2)食管鳞状细胞癌组织中PEDF蛋白表达与MVD的关系:29例PEDF表达阳性的食管鳞状细胞癌组织MVD均值是38.3793,而35例PEDF表达阴性的食管鳞状细胞癌组织MVD均值是43.4857,差异有统计学意义(P0.05)。结论:PEDF在食管鳞状细胞癌组织中表达降低,且其表达降低对食管鳞状细胞癌预后的判断可能有一定的参考价值。PEDF具有选择性的抗新生血管生成作用,使它成为抗肿瘤治疗的潜力分子,但其有效性、安全性、用药途径(包括合适的载体途径)都需要更多的研究进一步证实。
[Abstract]:Objective: to study the expression and clinical significance of pigment epithelium-derived factor epithelium-derived factor (PEDF) in esophageal squamous cell carcinoma (esophageal squamous cell carcinoma). To investigate the correlation between the expression of PEDF protein and microvessel density (MVD) in esophageal squamous cell carcinoma (SCC). Methods: from February 2016 to October 2016, cardiothoracic surgery was performed at Nanchong Central Hospital of Sichuan Province and affiliated Hospital of North Sichuan Medical College. There were 64 cases of esophageal squamous cell carcinoma and surrounding normal esophageal tissue. All the patients were not treated with radiotherapy, chemotherapy and other anti-tumor drugs before operation. The expression of PEDF mRNA in 64 esophageal squamous cell carcinoma tissues and the normal esophageal tissues around the same patient was detected by real-time quantitative PCR(real-time quantitative rrrrrrrrrrrrrtPCR.The expression of PEDF mRNA in 64 cases of esophageal squamous cell carcinoma and normal esophageal tissues around the same patient was detected. Immunohistochemistry was used to detect the expression of PEDF protein in 64 cases of esophageal squamous cell carcinoma and normal esophageal tissues around the same patient and the expression level of MVD in cancer tissue. Results: the real-time fluorescence quantitative PCR results showed that the expression of PEDF protein in the tissues of 64 cases of esophageal squamous cell carcinoma and the normal esophageal tissues around the same patient. PEDF mRNA were expressed in different intensity. The relative quantitative value of PEDF mRNA in esophageal squamous cell carcinoma was 0.77, which was significantly lower than that in matched normal esophageal tissue (1.04). The expression of PEDF protein in 29 cases of esophageal squamous cell carcinoma was negative in 29 cases of squamous cell carcinoma of esophagus, and the expression of PEDF protein was negative in 35 cases, the results of immunohistochemistry showed that the expression of PEDF protein was negative in 29 cases of esophageal squamous cell carcinoma, and the expression of PEDF protein was negative in 29 cases of squamous cell carcinoma of esophagus. The positive expression rate of PEDF protein was 45.31%, while the positive expression of PEDF protein was negative in 21 cases in 43 normal esophageal tissues. The positive expression rate was 67.19, and the difference between the two groups was statistically significant (P 0.05). There was no significant correlation between the expression of PEDF protein and the age and sex of the patients, but there was no significant correlation between the expression of PEDF protein and the clinical stage of the tumor. The degree of tissue differentiation is related to lymph node metastasis, and the lower the degree of differentiation of tumor tissue is, Relationship between PEDF protein expression and MVD in esophageal squamous Cell carcinoma tissues with late staging or lymph Node Metastasis MVD mean value of MVD in 29 cases of esophageal squamous Cell carcinoma with positive PEDF expression was 38.3793, but negative in 35 cases. The average value of MVD in esophageal squamous cell carcinoma was 43.4857, and the difference was statistically significant (P 0.05). Conclusion the expression of MVD in esophageal squamous cell carcinoma is decreased. The lower expression of PEDF may be useful in judging the prognosis of esophageal squamous cell carcinoma. PEDF has a selective anti-angiogenic effect, which makes it a potential molecule for antitumor therapy, but it is effective and safe. The drug pathway (including the appropriate carrier pathway) needs more research to further confirm.
【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.1

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